Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface
Lyme disease is a vector-borne illness caused by spirochetes belonging to the <i>Borrelia burgdorferi</i> species group. These bacteria employ several mechanisms to survive within the vertebrate host, including evasion of the complement system. In this study, we examine the protection ag...
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MDPI AG
2024-11-01
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| author | Tobias Jakobsson Pär Comstedt Sven Bergström Johan Normark |
| author_facet | Tobias Jakobsson Pär Comstedt Sven Bergström Johan Normark |
| author_sort | Tobias Jakobsson |
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| description | Lyme disease is a vector-borne illness caused by spirochetes belonging to the <i>Borrelia burgdorferi</i> species group. These bacteria employ several mechanisms to survive within the vertebrate host, including evasion of the complement system. In this study, we examine the protection against human serum killing by the binding of host complement regulators C4b-binding protein (C4BP) and factor H (FH) to the bacterial surface of <i>B. burgdorferi</i>. Via serum depletion of isolated complement regulators, we found that the absence of C4BP did not alter the survival of <i>B. burgdorferi</i> strain B31; however, the removal of FH increased the sensitivity of this strain to human serum as previously described. The <i>B. garinii</i> seabird-isolated strain Far04, on the other hand, did not bind any complement regulators of human origin and was serum-sensitive, indicating its special host species specificity. |
| format | Article |
| id | doaj-art-c0a3116475dc496b9c395f28f2d8939b |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-c0a3116475dc496b9c395f28f2d8939b2024-11-26T18:16:51ZengMDPI AGPathogens2076-08172024-11-01131197610.3390/pathogens13110976Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial SurfaceTobias Jakobsson0Pär Comstedt1Sven Bergström2Johan Normark3Department of Clinical Microbiology, Umeå University, 901 87 Umeå, SwedenEvaxion Biotech, Dr. Neergaards vej 5F, 2970 Hørsholm, DenmarkUCMR, Umeå Centre for Microbial Research, 901 87 Umeå, SwedenDepartment of Clinical Microbiology, Umeå University, 901 87 Umeå, SwedenLyme disease is a vector-borne illness caused by spirochetes belonging to the <i>Borrelia burgdorferi</i> species group. These bacteria employ several mechanisms to survive within the vertebrate host, including evasion of the complement system. In this study, we examine the protection against human serum killing by the binding of host complement regulators C4b-binding protein (C4BP) and factor H (FH) to the bacterial surface of <i>B. burgdorferi</i>. Via serum depletion of isolated complement regulators, we found that the absence of C4BP did not alter the survival of <i>B. burgdorferi</i> strain B31; however, the removal of FH increased the sensitivity of this strain to human serum as previously described. The <i>B. garinii</i> seabird-isolated strain Far04, on the other hand, did not bind any complement regulators of human origin and was serum-sensitive, indicating its special host species specificity.https://www.mdpi.com/2076-0817/13/11/976<i>Borrelia</i>C4b-binding proteincomplementserum survival |
| spellingShingle | Tobias Jakobsson Pär Comstedt Sven Bergström Johan Normark Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface Pathogens <i>Borrelia</i> C4b-binding protein complement serum survival |
| title | Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface |
| title_full | Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface |
| title_fullStr | Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface |
| title_full_unstemmed | Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface |
| title_short | Survival of <i>Borrelia burgdorferi</i> Strain B31 in Human Serum Is Not Dependent on C4BP Binding to the Bacterial Surface |
| title_sort | survival of i borrelia burgdorferi i strain b31 in human serum is not dependent on c4bp binding to the bacterial surface |
| topic | <i>Borrelia</i> C4b-binding protein complement serum survival |
| url | https://www.mdpi.com/2076-0817/13/11/976 |
| work_keys_str_mv | AT tobiasjakobsson survivalofiborreliaburgdorferiistrainb31inhumanserumisnotdependentonc4bpbindingtothebacterialsurface AT parcomstedt survivalofiborreliaburgdorferiistrainb31inhumanserumisnotdependentonc4bpbindingtothebacterialsurface AT svenbergstrom survivalofiborreliaburgdorferiistrainb31inhumanserumisnotdependentonc4bpbindingtothebacterialsurface AT johannormark survivalofiborreliaburgdorferiistrainb31inhumanserumisnotdependentonc4bpbindingtothebacterialsurface |