Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa
Zinc, an essential micronutrient, is an important mediator of sperm function. Supplementation of zinc oxide nanoparticle (ZnONPs) to semen cryopreservation medium has shown beneficial effects on sperm function, mainly due to its antioxidant and membrane stabilization properties. However, the ZnONPs...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2024.2422648 |
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| author | Pooja Suresh Poojary Ajjappla Basavaraj Shreya Srinivas Mutalik Divya Sachdev Neetu K. Taneja Shamprasad Varija Raghu Renu Pasricha Hanumappa Ananda K. Pramoda R. Vani Lakshmi Satish Kumar Adiga Guruprasad Kalthur |
| author_facet | Pooja Suresh Poojary Ajjappla Basavaraj Shreya Srinivas Mutalik Divya Sachdev Neetu K. Taneja Shamprasad Varija Raghu Renu Pasricha Hanumappa Ananda K. Pramoda R. Vani Lakshmi Satish Kumar Adiga Guruprasad Kalthur |
| author_sort | Pooja Suresh Poojary |
| collection | DOAJ |
| description | Zinc, an essential micronutrient, is an important mediator of sperm function. Supplementation of zinc oxide nanoparticle (ZnONPs) to semen cryopreservation medium has shown beneficial effects on sperm function, mainly due to its antioxidant and membrane stabilization properties. However, the ZnONPs are known to be membrane impermeable. In the current study, we explored the beneficial properties of membrane permeable zinc oxide nanoparticles on semen cryopreservation outcome. For the study, 52 liquefied human ejaculates were cryopreserved with chitosan-coated ZnONPs and stored in liquid nitrogen for one week. Samples were thawed and assessed for the sperm functional characters and DNA integrity. Significantly higher motility (P < 0.05), prolonged survival (P < 0.05) and lower DNA damage (P < 0.05) was observed in spermatozoa of semen samples cryopreserved with chitosan-coated ZnONPs. In conclusion, the presence of chitosan-coated ZnONPs in the ejaculate prior to cryopreservation increases the survival and decreases DNA damage without compromising with the functional competence of spermatozoa. |
| format | Article |
| id | doaj-art-c0063e2c664e469ea02417b48d9b37dc |
| institution | Kabale University |
| issn | 1658-3655 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-c0063e2c664e469ea02417b48d9b37dc2024-12-17T11:38:48ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552024-12-0118110.1080/16583655.2024.2422648Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoaPooja Suresh Poojary0Ajjappla Basavaraj Shreya1Srinivas Mutalik2Divya Sachdev3Neetu K. Taneja4Shamprasad Varija Raghu5Renu Pasricha6Hanumappa Ananda7K. Pramoda8R. Vani Lakshmi9Satish Kumar Adiga10Guruprasad Kalthur11Centre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, IndiaDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, IndiaDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, IndiaDepartment of Basic and Applied Sciences, National Institute of Food Technology Entrepreneurship and Management, Kundli, IndiaDepartment of Basic and Applied Sciences, National Institute of Food Technology Entrepreneurship and Management, Kundli, IndiaNeurogenetics Lab, Department of Applied Zoology, Mangalore University, Mangalagangotri, IndiaElectron Microscopy Core Technology Platforms Operations, New York University Abu Dhabi, Abu Dhabi, United Arab EmiratesDivision of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, IndiaCentre for Nano and Material Sciences, Jain Global Campus, Jain University, Bengaluru, IndiaDepartment of Data Science, Prasanna School of Public Health, Manipal Academy of Higher Education, Manipal, IndiaCentre of Excellence in Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, IndiaDivision of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, IndiaZinc, an essential micronutrient, is an important mediator of sperm function. Supplementation of zinc oxide nanoparticle (ZnONPs) to semen cryopreservation medium has shown beneficial effects on sperm function, mainly due to its antioxidant and membrane stabilization properties. However, the ZnONPs are known to be membrane impermeable. In the current study, we explored the beneficial properties of membrane permeable zinc oxide nanoparticles on semen cryopreservation outcome. For the study, 52 liquefied human ejaculates were cryopreserved with chitosan-coated ZnONPs and stored in liquid nitrogen for one week. Samples were thawed and assessed for the sperm functional characters and DNA integrity. Significantly higher motility (P < 0.05), prolonged survival (P < 0.05) and lower DNA damage (P < 0.05) was observed in spermatozoa of semen samples cryopreserved with chitosan-coated ZnONPs. In conclusion, the presence of chitosan-coated ZnONPs in the ejaculate prior to cryopreservation increases the survival and decreases DNA damage without compromising with the functional competence of spermatozoa.https://www.tandfonline.com/doi/10.1080/16583655.2024.2422648Acrosome damagemotilitytripolyphosphatezeta potentialsperm chromatin dispersion testmitochondrial potential |
| spellingShingle | Pooja Suresh Poojary Ajjappla Basavaraj Shreya Srinivas Mutalik Divya Sachdev Neetu K. Taneja Shamprasad Varija Raghu Renu Pasricha Hanumappa Ananda K. Pramoda R. Vani Lakshmi Satish Kumar Adiga Guruprasad Kalthur Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa Journal of Taibah University for Science Acrosome damage motility tripolyphosphate zeta potential sperm chromatin dispersion test mitochondrial potential |
| title | Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa |
| title_full | Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa |
| title_fullStr | Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa |
| title_full_unstemmed | Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa |
| title_short | Mitigating effect of chitosan-coated zinc oxide nanoparticles on cryopreservation-induced DNA damage in human spermatozoa |
| title_sort | mitigating effect of chitosan coated zinc oxide nanoparticles on cryopreservation induced dna damage in human spermatozoa |
| topic | Acrosome damage motility tripolyphosphate zeta potential sperm chromatin dispersion test mitochondrial potential |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2024.2422648 |
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