A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry
The structural complexity and limited standards for catechin derivatives in black tea present significant analytical challenges in tea research. This study aimed to develop a systematic approach to extract and analyze catechins and derivatives in Ceylon black tea from four Sri Lankan regions (Dimbul...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525004687 |
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| author | Shanbo Zhang Xinyao Long Yiwen Xing Kim-Huey Ee Rui Min Vivian Goh Yunle Huang Aileen Pua Lionel Jublot Shao Quan Liu Bin Yu |
| author_facet | Shanbo Zhang Xinyao Long Yiwen Xing Kim-Huey Ee Rui Min Vivian Goh Yunle Huang Aileen Pua Lionel Jublot Shao Quan Liu Bin Yu |
| author_sort | Shanbo Zhang |
| collection | DOAJ |
| description | The structural complexity and limited standards for catechin derivatives in black tea present significant analytical challenges in tea research. This study aimed to develop a systematic approach to extract and analyze catechins and derivatives in Ceylon black tea from four Sri Lankan regions (Dimbula, Nuwara Eliya, Uva, Ruhuna) using optimized extraction conditions, LC-QQQ/MS, and antioxidant assays. A design of experiment (DoE) optimized solvent (0–100 % acetone-water), temperature (30–60 °C), and time (0–150 min), identifying 49.8 % acetone, 123.6 min with sonication, and 49.8 °C as optimal (desirability 0.743). Twelve catechin-related compounds were identified by LC-QQQ/MS, including theaflavins and tentatively annotated derivatives. TPC, TFC, DPPH, and ABTS assays showed Nuwara Eliya tea had the highest antioxidant capacity, linked to elevated polyphenols and flavonoids. This workflow offers a robust framework for studying catechin derivatives with limited standards, offering a practical solution for black tea research and quality assessment. |
| format | Article |
| id | doaj-art-bf60e8f6b0e44dfba072e5a76f3f6d7c |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-bf60e8f6b0e44dfba072e5a76f3f6d7c2025-08-20T03:26:39ZengElsevierFood Chemistry: X2590-15752025-05-012810262110.1016/j.fochx.2025.102621A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometryShanbo Zhang0Xinyao Long1Yiwen Xing2Kim-Huey Ee3Rui Min Vivian Goh4Yunle Huang5Aileen Pua6Lionel Jublot7Shao Quan Liu8Bin Yu9Department of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, 117542, SingaporeMane SEA PTE LTD, 3 Biopolis Drive, #07-17/18/19 Synapse, 138623, SingaporeMane SEA PTE LTD, 3 Biopolis Drive, #07-17/18/19 Synapse, 138623, SingaporeMane SEA PTE LTD, 3 Biopolis Drive, #07-17/18/19 Synapse, 138623, SingaporeMane SEA PTE LTD, 3 Biopolis Drive, #07-17/18/19 Synapse, 138623, SingaporeMane SEA PTE LTD, 3 Biopolis Drive, #07-17/18/19 Synapse, 138623, SingaporeDepartment of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, 117542, Singapore; Corresponding authors.Mane SEA PTE LTD, 3 Biopolis Drive, #07-17/18/19 Synapse, 138623, Singapore; Corresponding authors.The structural complexity and limited standards for catechin derivatives in black tea present significant analytical challenges in tea research. This study aimed to develop a systematic approach to extract and analyze catechins and derivatives in Ceylon black tea from four Sri Lankan regions (Dimbula, Nuwara Eliya, Uva, Ruhuna) using optimized extraction conditions, LC-QQQ/MS, and antioxidant assays. A design of experiment (DoE) optimized solvent (0–100 % acetone-water), temperature (30–60 °C), and time (0–150 min), identifying 49.8 % acetone, 123.6 min with sonication, and 49.8 °C as optimal (desirability 0.743). Twelve catechin-related compounds were identified by LC-QQQ/MS, including theaflavins and tentatively annotated derivatives. TPC, TFC, DPPH, and ABTS assays showed Nuwara Eliya tea had the highest antioxidant capacity, linked to elevated polyphenols and flavonoids. This workflow offers a robust framework for studying catechin derivatives with limited standards, offering a practical solution for black tea research and quality assessment.http://www.sciencedirect.com/science/article/pii/S2590157525004687Ceylon black teaCatechin derivativesDesign of experimentLC-QQQ/MS |
| spellingShingle | Shanbo Zhang Xinyao Long Yiwen Xing Kim-Huey Ee Rui Min Vivian Goh Yunle Huang Aileen Pua Lionel Jublot Shao Quan Liu Bin Yu A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry Food Chemistry: X Ceylon black tea Catechin derivatives Design of experiment LC-QQQ/MS |
| title | A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry |
| title_full | A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry |
| title_fullStr | A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry |
| title_full_unstemmed | A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry |
| title_short | A systematic approach to analyzing catechins and catechin derivatives in Ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry |
| title_sort | systematic approach to analyzing catechins and catechin derivatives in ceylon black tea using liquid chromatography coupled with triple quadrupole mass spectrometry |
| topic | Ceylon black tea Catechin derivatives Design of experiment LC-QQQ/MS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525004687 |
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