Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation

Replicating the intricate architecture of native tissues remains a significant challenge in tissue engineering. Ice-templated biomimetic scaffolds possess controlled porosity that conveniently resembles the native parenchyma of many tissues. In this study, we investigate the relationship between the...

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Main Authors: Gengyao Wei, Ruth E. Cameron, Serena M. Best
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Materials Today Advances
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590049824000808
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author Gengyao Wei
Ruth E. Cameron
Serena M. Best
author_facet Gengyao Wei
Ruth E. Cameron
Serena M. Best
author_sort Gengyao Wei
collection DOAJ
description Replicating the intricate architecture of native tissues remains a significant challenge in tissue engineering. Ice-templated biomimetic scaffolds possess controlled porosity that conveniently resembles the native parenchyma of many tissues. In this study, we investigate the relationship between the porous architecture of lyophilised collagen scaffolds and key processing parameters during production. We establish a predictive model that correlates specific lyophilisation conditions with the resulting pore sizes. Systematic variations in the freeze-drying conditions resulted in scaffolds with average pore sizes ranging from 46 μm to 251 μm, effectively matching the length scale of extracellular matrix features found in native tissues. We introduce the concept of heat flux density (HFD) at equilibrium as a metric for quantifying latent heat extraction efficiency during the freezing process. Our findings reveal a power law relationship between HFD at equilibrium and pore size, with an exponent of −0.44. This approach provides a non-destructive and non-intrusive method for precisely controlling pore architecture, advancing the potential for creating scaffolds that closely emulate the complex structures of native tissues.
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spelling doaj-art-bf13a2c4d7df4c1daf58d49cc35b7c322024-12-14T06:32:33ZengElsevierMaterials Today Advances2590-04982024-12-0124100543Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulationGengyao Wei0Ruth E. Cameron1Serena M. Best2Corresponding author.; Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge, CB3 0FT, UKDepartment of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge, CB3 0FT, UKDepartment of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge, CB3 0FT, UKReplicating the intricate architecture of native tissues remains a significant challenge in tissue engineering. Ice-templated biomimetic scaffolds possess controlled porosity that conveniently resembles the native parenchyma of many tissues. In this study, we investigate the relationship between the porous architecture of lyophilised collagen scaffolds and key processing parameters during production. We establish a predictive model that correlates specific lyophilisation conditions with the resulting pore sizes. Systematic variations in the freeze-drying conditions resulted in scaffolds with average pore sizes ranging from 46 μm to 251 μm, effectively matching the length scale of extracellular matrix features found in native tissues. We introduce the concept of heat flux density (HFD) at equilibrium as a metric for quantifying latent heat extraction efficiency during the freezing process. Our findings reveal a power law relationship between HFD at equilibrium and pore size, with an exponent of −0.44. This approach provides a non-destructive and non-intrusive method for precisely controlling pore architecture, advancing the potential for creating scaffolds that closely emulate the complex structures of native tissues.http://www.sciencedirect.com/science/article/pii/S2590049824000808Freeze-dryingIce-templatingLyophilisationCollagen scaffoldsHeat flux density
spellingShingle Gengyao Wei
Ruth E. Cameron
Serena M. Best
Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation
Materials Today Advances
Freeze-drying
Ice-templating
Lyophilisation
Collagen scaffolds
Heat flux density
title Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation
title_full Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation
title_fullStr Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation
title_full_unstemmed Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation
title_short Predictive control of pore architecture in ice-templated scaffolds via heat flux density modulation
title_sort predictive control of pore architecture in ice templated scaffolds via heat flux density modulation
topic Freeze-drying
Ice-templating
Lyophilisation
Collagen scaffolds
Heat flux density
url http://www.sciencedirect.com/science/article/pii/S2590049824000808
work_keys_str_mv AT gengyaowei predictivecontrolofporearchitectureinicetemplatedscaffoldsviaheatfluxdensitymodulation
AT ruthecameron predictivecontrolofporearchitectureinicetemplatedscaffoldsviaheatfluxdensitymodulation
AT serenambest predictivecontrolofporearchitectureinicetemplatedscaffoldsviaheatfluxdensitymodulation