Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice
Background: At present, the treatment for allergic rhinitis (AR) is only limited to symptom relief, and AR is not able be cured. It is important to find new therapeutic regimens for AR. Objective: To explore the effect of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) on AR in mice. Meth...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024173719 |
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| author | Xiaojie Xu Chang Li Qin Qin Feiyan Han Ying Wang |
| author_facet | Xiaojie Xu Chang Li Qin Qin Feiyan Han Ying Wang |
| author_sort | Xiaojie Xu |
| collection | DOAJ |
| description | Background: At present, the treatment for allergic rhinitis (AR) is only limited to symptom relief, and AR is not able be cured. It is important to find new therapeutic regimens for AR. Objective: To explore the effect of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) on AR in mice. Methods: Primary human AMSCs were cultured up to fourth or fifth passage AMSCs which were used to extract AMSC-exos by ultracentrifugation. The AMSC-exos were identified in size, morphology and surface marker proteins. AR mouse models were made, and then effects of the AMSC-exos on AR mouse models were observed by injection of AMSC-exos into their tail veins. A total of 21 mice were divided into normal mice with injection of phosphate buffered saline (PBS) group (Normal + PBS group, 7 mice), AR mice with injection of AMSC-exos group (AR + AMSC-exos group, 7 mice) and AR mice with injection of PBS (AR + PBS group, 7 mice). After injection of AMSC-exos and PBS, symptoms were observed in mice and inflammatory factors including IL-4, IL-5, IFN- γ and Ig-E were determined. Mice were sacrificed, nasal mucosa was collected for HE staining and qRT-PCR. Results: After injection of AMSC-exos, compared with the AR + PBS group, symptoms were significantly improved, inflammatory factor levels were significantly decreased, disturbed and thickened mucosal layer was relieved, and the numbers of goblet cells and eosinophils were reduced in the AR + AMSC-exos group. Conclusion: AMSC-exos can improve symptoms and inflammatory level in the AR mice. |
| format | Article |
| id | doaj-art-be6372442c5547b28a955a7211c7a7eb |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-be6372442c5547b28a955a7211c7a7eb2025-01-17T04:50:58ZengElsevierHeliyon2405-84402025-01-01111e41340Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in miceXiaojie Xu0Chang Li1Qin Qin2Feiyan Han3Ying Wang4Nasal Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, ChinaNasal Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, ChinaNasal Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, ChinaNasal Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, ChinaCorresponding author. Number one, Constructive East Road, Erqi District, Zhengzhou, 450052, China.; Nasal Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, ChinaBackground: At present, the treatment for allergic rhinitis (AR) is only limited to symptom relief, and AR is not able be cured. It is important to find new therapeutic regimens for AR. Objective: To explore the effect of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) on AR in mice. Methods: Primary human AMSCs were cultured up to fourth or fifth passage AMSCs which were used to extract AMSC-exos by ultracentrifugation. The AMSC-exos were identified in size, morphology and surface marker proteins. AR mouse models were made, and then effects of the AMSC-exos on AR mouse models were observed by injection of AMSC-exos into their tail veins. A total of 21 mice were divided into normal mice with injection of phosphate buffered saline (PBS) group (Normal + PBS group, 7 mice), AR mice with injection of AMSC-exos group (AR + AMSC-exos group, 7 mice) and AR mice with injection of PBS (AR + PBS group, 7 mice). After injection of AMSC-exos and PBS, symptoms were observed in mice and inflammatory factors including IL-4, IL-5, IFN- γ and Ig-E were determined. Mice were sacrificed, nasal mucosa was collected for HE staining and qRT-PCR. Results: After injection of AMSC-exos, compared with the AR + PBS group, symptoms were significantly improved, inflammatory factor levels were significantly decreased, disturbed and thickened mucosal layer was relieved, and the numbers of goblet cells and eosinophils were reduced in the AR + AMSC-exos group. Conclusion: AMSC-exos can improve symptoms and inflammatory level in the AR mice.http://www.sciencedirect.com/science/article/pii/S2405844024173719Adipose mesenchymal stem cell-derived exosomesExosomesImmunoloregulationAllergic rhinitisIL-4IL-5 |
| spellingShingle | Xiaojie Xu Chang Li Qin Qin Feiyan Han Ying Wang Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice Heliyon Adipose mesenchymal stem cell-derived exosomes Exosomes Immunoloregulation Allergic rhinitis IL-4 IL-5 |
| title | Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice |
| title_full | Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice |
| title_fullStr | Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice |
| title_full_unstemmed | Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice |
| title_short | Effect of adipose mesenchymal stem cell-derived exosomes on allergic rhinitis in mice |
| title_sort | effect of adipose mesenchymal stem cell derived exosomes on allergic rhinitis in mice |
| topic | Adipose mesenchymal stem cell-derived exosomes Exosomes Immunoloregulation Allergic rhinitis IL-4 IL-5 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024173719 |
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