Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence
Keratinocyte stress, caused by various intrinsic and extrinsic factors, contributes to the overall aging process. D-galactose-induced metabolic/oxidative stress is a commonly used <i>in vitro</i> model for studying premature aging. Due to their rich composition of bioactive molecules tha...
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MDPI AG
2025-06-01
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| author | Mirjana Nacka-Aleksić Andrea Pirković Aleksandra Vilotić Maja Kosanović Dragana Dekanski Janko Legner Milica Jovanović Krivokuća |
| author_facet | Mirjana Nacka-Aleksić Andrea Pirković Aleksandra Vilotić Maja Kosanović Dragana Dekanski Janko Legner Milica Jovanović Krivokuća |
| author_sort | Mirjana Nacka-Aleksić |
| collection | DOAJ |
| description | Keratinocyte stress, caused by various intrinsic and extrinsic factors, contributes to the overall aging process. D-galactose-induced metabolic/oxidative stress is a commonly used <i>in vitro</i> model for studying premature aging. Due to their rich composition of bioactive molecules that influence critical pathways in cellular aging and rejuvenation, placental derivatives have a well-established history in anti-aging skincare and therapy. However, trophoblast-derived extracellular vesicle (TEV) effects on D-galactose-induced premature aging in keratinocytes have not been investigated yet. TEV pretreatment for 24 h enhanced cellular resilience against D-galactose-induced stress, judging by the downregulated expression of senescence- and stress-associated markers (p19 and p21, HIF-1α, mTOR), and reduced production of reactive oxygen species and DNA damage. Additionally, TEV pretreatment enhanced keratinocyte proliferation and integrin-β1 subunit expression upon D-galactose exposure, most likely contributing to more efficient wound closure. In conclusion, this study underscores the potential of TEVs to modify expression of stress- and senescence-related proteins in keratinocytes and improve their wound healing properties. Their regenerative and protective characteristics position TEVs as promising candidates for developing innovative procedures to address skin conditions related to premature aging. |
| format | Article |
| id | doaj-art-be2b5fc8af6e46a68e169b6ffe043a1c |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-be2b5fc8af6e46a68e169b6ffe043a1c2025-08-20T03:16:18ZengMDPI AGLife2075-17292025-06-0115691810.3390/life15060918Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and SenescenceMirjana Nacka-Aleksić0Andrea Pirković1Aleksandra Vilotić2Maja Kosanović3Dragana Dekanski4Janko Legner5Milica Jovanović Krivokuća6University of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Banatska 31b, 11080 Zemun, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Banatska 31b, 11080 Zemun, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Banatska 31b, 11080 Zemun, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Immunology and Immunoparasitology, Banatska 31b, 11080 Zemun, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Banatska 31b, 11080 Zemun, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Banatska 31b, 11080 Zemun, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Banatska 31b, 11080 Zemun, SerbiaKeratinocyte stress, caused by various intrinsic and extrinsic factors, contributes to the overall aging process. D-galactose-induced metabolic/oxidative stress is a commonly used <i>in vitro</i> model for studying premature aging. Due to their rich composition of bioactive molecules that influence critical pathways in cellular aging and rejuvenation, placental derivatives have a well-established history in anti-aging skincare and therapy. However, trophoblast-derived extracellular vesicle (TEV) effects on D-galactose-induced premature aging in keratinocytes have not been investigated yet. TEV pretreatment for 24 h enhanced cellular resilience against D-galactose-induced stress, judging by the downregulated expression of senescence- and stress-associated markers (p19 and p21, HIF-1α, mTOR), and reduced production of reactive oxygen species and DNA damage. Additionally, TEV pretreatment enhanced keratinocyte proliferation and integrin-β1 subunit expression upon D-galactose exposure, most likely contributing to more efficient wound closure. In conclusion, this study underscores the potential of TEVs to modify expression of stress- and senescence-related proteins in keratinocytes and improve their wound healing properties. Their regenerative and protective characteristics position TEVs as promising candidates for developing innovative procedures to address skin conditions related to premature aging.https://www.mdpi.com/2075-1729/15/6/918skinagingplacentawound healingregenerative medicine |
| spellingShingle | Mirjana Nacka-Aleksić Andrea Pirković Aleksandra Vilotić Maja Kosanović Dragana Dekanski Janko Legner Milica Jovanović Krivokuća Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence Life skin aging placenta wound healing regenerative medicine |
| title | Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence |
| title_full | Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence |
| title_fullStr | Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence |
| title_full_unstemmed | Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence |
| title_short | Trophoblast Extracellular Vesicles as Modulators of Keratinocyte Stress Response and Senescence |
| title_sort | trophoblast extracellular vesicles as modulators of keratinocyte stress response and senescence |
| topic | skin aging placenta wound healing regenerative medicine |
| url | https://www.mdpi.com/2075-1729/15/6/918 |
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