Measuring plant cysteine oxidase interactions with substrates using intrinsic tryptophan fluorescence
Abstract Plant Cysteine Oxidases (PCOs) are oxygen-sensing enyzmes that catalyse oxidation of cysteinyl residues at the N-termini of target proteins, triggering their degradation via the N-degron pathway. PCO oxygen sensitivity means that in low oxygen conditions (hypoxia), their activity reduces an...
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Main Authors: | Dona M. Gunawardana, Daisy A. Southern, Emily Flashman |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2024-12-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-024-83508-y |
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