Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner
Abstract The microbiota of mosquitoes influences many aspects of their biology, including developmental processes, mating and sexual reproduction, immune functions, and refractoriness to pathogens. Here, we considered their role in resistance against insecticides. In particular, we assessed how larv...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-78288-4 |
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| author | Luís M. Silva Gwendoline Acerbi Marine Amann Jacob C. Koella |
| author_facet | Luís M. Silva Gwendoline Acerbi Marine Amann Jacob C. Koella |
| author_sort | Luís M. Silva |
| collection | DOAJ |
| description | Abstract The microbiota of mosquitoes influences many aspects of their biology, including developmental processes, mating and sexual reproduction, immune functions, and refractoriness to pathogens. Here, we considered their role in resistance against insecticides. In particular, we assessed how larval infection of a permethrin-resistant and a sensitive colony of Anopheles gambiae by four strains belonging to three different Pseudomonas species affects several life history traits and the impact of the insecticide on adult mortality. Our data showed that all four Pseudomonas strains persisted in adults until death. The bacteria increased the likelihood that mosquitoes survived 24 h after exposure to permethrin by up to twofold. The impact of the bacteria depended on the bacterial strains and the mosquito colony: in the resistant colony, all bacteria increased survival by about twofold, while in the sensitive colony, only two of the four strains increased survival. The benefit concerning insecticide resistance came with little to no impact on the other traits (i.e., larval mortality, developmental time and adult longevity). Altogether, our results highlight the importance of considering environmental microbial exposure and mosquito microbial communities in epidemiological and vector-control studies, while also suggesting a possible role for Pseudomonas spp. as a symbiont in A. gambiae. |
| format | Article |
| id | doaj-art-be23d935e1f34dbd99f7c9df44a0e548 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-be23d935e1f34dbd99f7c9df44a0e5482024-12-01T12:22:37ZengNature PortfolioScientific Reports2045-23222024-11-011411910.1038/s41598-024-78288-4Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent mannerLuís M. Silva0Gwendoline Acerbi1Marine Amann2Jacob C. Koella3Institute of Biology, University of NeuchâtelInstitute of Biology, University of NeuchâtelInstitute of Biology, University of NeuchâtelInstitute of Biology, University of NeuchâtelAbstract The microbiota of mosquitoes influences many aspects of their biology, including developmental processes, mating and sexual reproduction, immune functions, and refractoriness to pathogens. Here, we considered their role in resistance against insecticides. In particular, we assessed how larval infection of a permethrin-resistant and a sensitive colony of Anopheles gambiae by four strains belonging to three different Pseudomonas species affects several life history traits and the impact of the insecticide on adult mortality. Our data showed that all four Pseudomonas strains persisted in adults until death. The bacteria increased the likelihood that mosquitoes survived 24 h after exposure to permethrin by up to twofold. The impact of the bacteria depended on the bacterial strains and the mosquito colony: in the resistant colony, all bacteria increased survival by about twofold, while in the sensitive colony, only two of the four strains increased survival. The benefit concerning insecticide resistance came with little to no impact on the other traits (i.e., larval mortality, developmental time and adult longevity). Altogether, our results highlight the importance of considering environmental microbial exposure and mosquito microbial communities in epidemiological and vector-control studies, while also suggesting a possible role for Pseudomonas spp. as a symbiont in A. gambiae.https://doi.org/10.1038/s41598-024-78288-4 |
| spellingShingle | Luís M. Silva Gwendoline Acerbi Marine Amann Jacob C. Koella Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner Scientific Reports |
| title | Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner |
| title_full | Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner |
| title_fullStr | Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner |
| title_full_unstemmed | Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner |
| title_short | Exposure to Pseudomonas spp. increases Anopheles gambiae insecticide resistance in a host-dependent manner |
| title_sort | exposure to pseudomonas spp increases anopheles gambiae insecticide resistance in a host dependent manner |
| url | https://doi.org/10.1038/s41598-024-78288-4 |
| work_keys_str_mv | AT luismsilva exposuretopseudomonassppincreasesanophelesgambiaeinsecticideresistanceinahostdependentmanner AT gwendolineacerbi exposuretopseudomonassppincreasesanophelesgambiaeinsecticideresistanceinahostdependentmanner AT marineamann exposuretopseudomonassppincreasesanophelesgambiaeinsecticideresistanceinahostdependentmanner AT jacobckoella exposuretopseudomonassppincreasesanophelesgambiaeinsecticideresistanceinahostdependentmanner |