Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC

Plants are the most ubiquitous sources of bioactive organic compounds on earth. Active metabolites such as phytochemicals from medicinal plants have been investigated for the development of cutting-edge and biodegradable potent drugs as an alternative to ineffective modern treatment. The medicinal...

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Main Authors: Adil Jamal, Amina Arif, Muhammad Naveed Shahid
Format: Article
Language:English
Published: Universidade Estadual de Maringá 2024-12-01
Series:Acta Scientiarum: Biological Sciences
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Online Access:https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/72215
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author Adil Jamal
Amina Arif
Muhammad Naveed Shahid
author_facet Adil Jamal
Amina Arif
Muhammad Naveed Shahid
author_sort Adil Jamal
collection DOAJ
description Plants are the most ubiquitous sources of bioactive organic compounds on earth. Active metabolites such as phytochemicals from medicinal plants have been investigated for the development of cutting-edge and biodegradable potent drugs as an alternative to ineffective modern treatment. The medicinal plants Calotropis gigantea and Moringa oleifera contain biochemical constituents that may have therapeutic qualities. The bioactive components in C. gigantea and M. oleifera leaves were analyzed using HPLC. HPLC analysis of several leaf fractions unveils the presence of quercetin, gallic acid, coumaric acid, caffeic acid, chlorogenic acid, ferulic acid, cinnamic acid, sinapinic acid, and vanillic acid in both plants. Furthermore, phytochemical study of these plants revealed the presence of alkaloids, flavonoids, saponins, tannins, terpenoids, and glycosides in different solvents. The identified chemicals have reported antioxidant and antitumor properties. The current study's findings suggest that C. gigantea and M. oleifera could be used as a source for a credible antioxidant agent that might later be isolated and used as lead candidates for the fabrication of antioxidant drugs that help stop or limit free radical damage and counteract oxidative stress, thereby preventing a variety of chronic and degenerative diseases.
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spelling doaj-art-bde6753349cb4f309fe16c3b77a117b12024-12-16T18:55:10ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2024-12-0146110.4025/actascibiolsci.v46i1.72215Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLCAdil Jamal0Amina Arif1Muhammad Naveed Shahid2The University of FaisalabadUniversity of Central PunjabUniversity of Education Plants are the most ubiquitous sources of bioactive organic compounds on earth. Active metabolites such as phytochemicals from medicinal plants have been investigated for the development of cutting-edge and biodegradable potent drugs as an alternative to ineffective modern treatment. The medicinal plants Calotropis gigantea and Moringa oleifera contain biochemical constituents that may have therapeutic qualities. The bioactive components in C. gigantea and M. oleifera leaves were analyzed using HPLC. HPLC analysis of several leaf fractions unveils the presence of quercetin, gallic acid, coumaric acid, caffeic acid, chlorogenic acid, ferulic acid, cinnamic acid, sinapinic acid, and vanillic acid in both plants. Furthermore, phytochemical study of these plants revealed the presence of alkaloids, flavonoids, saponins, tannins, terpenoids, and glycosides in different solvents. The identified chemicals have reported antioxidant and antitumor properties. The current study's findings suggest that C. gigantea and M. oleifera could be used as a source for a credible antioxidant agent that might later be isolated and used as lead candidates for the fabrication of antioxidant drugs that help stop or limit free radical damage and counteract oxidative stress, thereby preventing a variety of chronic and degenerative diseases. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/72215acetic acid; aqueous chloroform; Calotropis; phytochemical; HPLC; Moringa.
spellingShingle Adil Jamal
Amina Arif
Muhammad Naveed Shahid
Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC
Acta Scientiarum: Biological Sciences
acetic acid; aqueous chloroform; Calotropis; phytochemical; HPLC; Moringa.
title Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC
title_full Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC
title_fullStr Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC
title_full_unstemmed Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC
title_short Identification of bioactive constituents in indigenously grown Calotropis gigantea and Moringa oleifera fractions by HPLC
title_sort identification of bioactive constituents in indigenously grown calotropis gigantea and moringa oleifera fractions by hplc
topic acetic acid; aqueous chloroform; Calotropis; phytochemical; HPLC; Moringa.
url https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/72215
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AT aminaarif identificationofbioactiveconstituentsinindigenouslygrowncalotropisgiganteaandmoringaoleiferafractionsbyhplc
AT muhammadnaveedshahid identificationofbioactiveconstituentsinindigenouslygrowncalotropisgiganteaandmoringaoleiferafractionsbyhplc