Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures

Abstract Background The application of a biochar in agronomical soil offers a dual benefit of improving soil quality and sustainable waste recycling. However, utilizing new organic waste sources requires exploring the biochar’s production conditions and application parameters. Woodchips (W) and bone...

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Main Authors: Martina Kracmarova-Farren, Eliska Alexova, Anezka Kodatova, Filip Mercl, Jirina Szakova, Pavel Tlustos, Katerina Demnerova, Hana Stiborova
Format: Article
Language:English
Published: BMC 2024-11-01
Series:Environmental Microbiome
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Online Access:https://doi.org/10.1186/s40793-024-00631-z
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author Martina Kracmarova-Farren
Eliska Alexova
Anezka Kodatova
Filip Mercl
Jirina Szakova
Pavel Tlustos
Katerina Demnerova
Hana Stiborova
author_facet Martina Kracmarova-Farren
Eliska Alexova
Anezka Kodatova
Filip Mercl
Jirina Szakova
Pavel Tlustos
Katerina Demnerova
Hana Stiborova
author_sort Martina Kracmarova-Farren
collection DOAJ
description Abstract Background The application of a biochar in agronomical soil offers a dual benefit of improving soil quality and sustainable waste recycling. However, utilizing new organic waste sources requires exploring the biochar’s production conditions and application parameters. Woodchips (W) and bone-meat residues (BM) after mechanical deboning from a poultry slaughterhouse were subjected to pyrolysis at 300 °C and 500 °C and applied to cambisol and luvisol soils at ratios of 2% and 5% (w/w). Results Initially, the impact of these biochar amendments on soil prokaryotes was studied over the course of one year. The influence of biochar variants was further studied on prokaryotes and fungi living in the soil, rhizosphere, and roots of Triticum aestivum L., as well as on soil enzymatic activity. Feedstock type, pyrolysis temperature, application dose, and soil type all played significant roles in shaping both soil and endophytic microbial communities. BM treated at a lower pyrolysis temperature of 300 °C increased the relative abundance of Pseudomonadota while causing a substantial decrease in soil microbial diversity. Conversely, BM prepared at 500 °C favored the growth of microbes known for their involvement in various nutrient cycles. The W biochar, especially when pyrolysed at 500 °C, notably affected microbial communities, particularly in acidic cambisol compared to luvisol. In cambisol, biochar treatments had a significant impact on prokaryotic root endophytes of T. aestivum L. Additionally, variations in prokaryotic community structure of the rhizosphere depended on the increasing distance from the root system (2, 4, and 6 mm). The BM biochar enhanced the activity of acid phosphatase, whereas the W biochar increased the activity of enzymes involved in the carbon cycle (β-glucosidase, β-xylosidase, and β-N-acetylglucosaminidase). Conclusions These results collectively suggest, that under appropriate production conditions, biochar can exert a positive influence on soil microorganisms, with their response closely tied to the biochar feedstock composition. Such insights are crucial for optimizing biochar application in agricultural practices to enhance soil health.
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spelling doaj-art-bdb794e7c7bb41edb535c267a61a7c922024-11-10T12:43:07ZengBMCEnvironmental Microbiome2524-63722024-11-0119111710.1186/s40793-024-00631-zBiochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperaturesMartina Kracmarova-Farren0Eliska Alexova1Anezka Kodatova2Filip Mercl3Jirina Szakova4Pavel Tlustos5Katerina Demnerova6Hana Stiborova7Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueDepartment of Agro-Environmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences PragueDepartment of Agro-Environmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences PragueDepartment of Agro-Environmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences PragueDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueAbstract Background The application of a biochar in agronomical soil offers a dual benefit of improving soil quality and sustainable waste recycling. However, utilizing new organic waste sources requires exploring the biochar’s production conditions and application parameters. Woodchips (W) and bone-meat residues (BM) after mechanical deboning from a poultry slaughterhouse were subjected to pyrolysis at 300 °C and 500 °C and applied to cambisol and luvisol soils at ratios of 2% and 5% (w/w). Results Initially, the impact of these biochar amendments on soil prokaryotes was studied over the course of one year. The influence of biochar variants was further studied on prokaryotes and fungi living in the soil, rhizosphere, and roots of Triticum aestivum L., as well as on soil enzymatic activity. Feedstock type, pyrolysis temperature, application dose, and soil type all played significant roles in shaping both soil and endophytic microbial communities. BM treated at a lower pyrolysis temperature of 300 °C increased the relative abundance of Pseudomonadota while causing a substantial decrease in soil microbial diversity. Conversely, BM prepared at 500 °C favored the growth of microbes known for their involvement in various nutrient cycles. The W biochar, especially when pyrolysed at 500 °C, notably affected microbial communities, particularly in acidic cambisol compared to luvisol. In cambisol, biochar treatments had a significant impact on prokaryotic root endophytes of T. aestivum L. Additionally, variations in prokaryotic community structure of the rhizosphere depended on the increasing distance from the root system (2, 4, and 6 mm). The BM biochar enhanced the activity of acid phosphatase, whereas the W biochar increased the activity of enzymes involved in the carbon cycle (β-glucosidase, β-xylosidase, and β-N-acetylglucosaminidase). Conclusions These results collectively suggest, that under appropriate production conditions, biochar can exert a positive influence on soil microorganisms, with their response closely tied to the biochar feedstock composition. Such insights are crucial for optimizing biochar application in agricultural practices to enhance soil health.https://doi.org/10.1186/s40793-024-00631-zBiocharSoil qualityOrganic waste recyclingMicrobial compositionEnzymatic activity
spellingShingle Martina Kracmarova-Farren
Eliska Alexova
Anezka Kodatova
Filip Mercl
Jirina Szakova
Pavel Tlustos
Katerina Demnerova
Hana Stiborova
Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures
Environmental Microbiome
Biochar
Soil quality
Organic waste recycling
Microbial composition
Enzymatic activity
title Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures
title_full Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures
title_fullStr Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures
title_full_unstemmed Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures
title_short Biochar-induced changes in soil microbial communities: a comparison of two feedstocks and pyrolysis temperatures
title_sort biochar induced changes in soil microbial communities a comparison of two feedstocks and pyrolysis temperatures
topic Biochar
Soil quality
Organic waste recycling
Microbial composition
Enzymatic activity
url https://doi.org/10.1186/s40793-024-00631-z
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