Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline
Choline is an important molecule in monitoring food safety and infant nutrition. Here, we report Ce nanogels synthesized by atom transfer radical polymerization (ATRP) employing Ce-coordinated acryloyl-lysine polymer brushes (Ce@SiO<sub>2</sub> NGs) as highly efficient cascade nanozymes...
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MDPI AG
2024-11-01
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| author | Trung Hieu Vu Byung Jo Yu Moon Il Kim |
| author_facet | Trung Hieu Vu Byung Jo Yu Moon Il Kim |
| author_sort | Trung Hieu Vu |
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| description | Choline is an important molecule in monitoring food safety and infant nutrition. Here, we report Ce nanogels synthesized by atom transfer radical polymerization (ATRP) employing Ce-coordinated acryloyl-lysine polymer brushes (Ce@SiO<sub>2</sub> NGs) as highly efficient cascade nanozymes for colorimetric detection of choline. The synthesized Ce@SiO<sub>2</sub> NGs demonstrated remarkable peroxidase-like activity with a porous exterior, which are essential to entrap choline oxidase (COx) to yield COx@Ce@SiO<sub>2</sub> NGs and construct a cascade reaction system to detect choline. Immobilized COx catalyzed the oxidation of choline in food samples to produce H<sub>2</sub>O<sub>2</sub>, which subsequently induced the oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue color signals. This method enabled the selective and sensitive detection of target choline with a satisfactory linear range of 4–400 μM, which is sufficient to analyze foodborne choline. The practical utility of the COx@Ce@SiO<sub>2</sub> NG-based assay was successfully validated to determine choline spiked in commercially available milk and infant formula with high accuracy and precision values. This approach provides a simple and affordable method of choline detection and has the potential to lead to more developments in ATRP-based nanozymes for diverse biosensing applications. |
| format | Article |
| id | doaj-art-bda1393cdd0f4386b5ca4bccf4ea076b |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-bda1393cdd0f4386b5ca4bccf4ea076b2024-12-27T14:14:08ZengMDPI AGBiosensors2079-63742024-11-01141256310.3390/bios14120563Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and CholineTrung Hieu Vu0Byung Jo Yu1Moon Il Kim2Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Republic of KoreaLow-Carbon Transition R&D Department, Research Institute of Sustainable Development Technology, Korea Institute of Industrial Technology (KITECH), Cheonan 31056, Republic of KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Republic of KoreaCholine is an important molecule in monitoring food safety and infant nutrition. Here, we report Ce nanogels synthesized by atom transfer radical polymerization (ATRP) employing Ce-coordinated acryloyl-lysine polymer brushes (Ce@SiO<sub>2</sub> NGs) as highly efficient cascade nanozymes for colorimetric detection of choline. The synthesized Ce@SiO<sub>2</sub> NGs demonstrated remarkable peroxidase-like activity with a porous exterior, which are essential to entrap choline oxidase (COx) to yield COx@Ce@SiO<sub>2</sub> NGs and construct a cascade reaction system to detect choline. Immobilized COx catalyzed the oxidation of choline in food samples to produce H<sub>2</sub>O<sub>2</sub>, which subsequently induced the oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue color signals. This method enabled the selective and sensitive detection of target choline with a satisfactory linear range of 4–400 μM, which is sufficient to analyze foodborne choline. The practical utility of the COx@Ce@SiO<sub>2</sub> NG-based assay was successfully validated to determine choline spiked in commercially available milk and infant formula with high accuracy and precision values. This approach provides a simple and affordable method of choline detection and has the potential to lead to more developments in ATRP-based nanozymes for diverse biosensing applications.https://www.mdpi.com/2079-6374/14/12/563ATRP-based nanogelscascade reactionperoxidase-like nanozymescholine detectionfood safety |
| spellingShingle | Trung Hieu Vu Byung Jo Yu Moon Il Kim Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline Biosensors ATRP-based nanogels cascade reaction peroxidase-like nanozymes choline detection food safety |
| title | Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline |
| title_full | Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline |
| title_fullStr | Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline |
| title_full_unstemmed | Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline |
| title_short | Choline Oxidase-Incorporated ATRP-Based Cerium Nanogels as Nanozymes for Colorimetric Detection of Hydrogen Peroxide and Choline |
| title_sort | choline oxidase incorporated atrp based cerium nanogels as nanozymes for colorimetric detection of hydrogen peroxide and choline |
| topic | ATRP-based nanogels cascade reaction peroxidase-like nanozymes choline detection food safety |
| url | https://www.mdpi.com/2079-6374/14/12/563 |
| work_keys_str_mv | AT trunghieuvu cholineoxidaseincorporatedatrpbasedceriumnanogelsasnanozymesforcolorimetricdetectionofhydrogenperoxideandcholine AT byungjoyu cholineoxidaseincorporatedatrpbasedceriumnanogelsasnanozymesforcolorimetricdetectionofhydrogenperoxideandcholine AT moonilkim cholineoxidaseincorporatedatrpbasedceriumnanogelsasnanozymesforcolorimetricdetectionofhydrogenperoxideandcholine |