Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance
Low freezing tolerance threatens the survival and productivity of perennial ryegrass under northern climate. In this study, we aimed to identify transcriptional changes in plants subjected to low and freezing temperatures as well as to elucidate differences between tolerant and sensitive genotypes....
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X24002513 |
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| author | Akhil Reddy Pashapu Gražina Statkevičiūtė Ferenz Sustek-Sánchez Mallikarjuna Rao Kovi Odd Arne Rognli Cecilia Sarmiento Nils Rostoks Kristina Jaškūnė |
| author_facet | Akhil Reddy Pashapu Gražina Statkevičiūtė Ferenz Sustek-Sánchez Mallikarjuna Rao Kovi Odd Arne Rognli Cecilia Sarmiento Nils Rostoks Kristina Jaškūnė |
| author_sort | Akhil Reddy Pashapu |
| collection | DOAJ |
| description | Low freezing tolerance threatens the survival and productivity of perennial ryegrass under northern climate. In this study, we aimed to identify transcriptional changes in plants subjected to low and freezing temperatures as well as to elucidate differences between tolerant and sensitive genotypes. Response to freezing stress was evaluated in a panel of 160 perennial ryegrass genotypes by measuring electrolyte leakage after exposure to -12 °C and -14 °C for 24 h. Two tolerant and two sensitive genotypes were selected for the transcriptome analysis. Crown tissue samples were collected at six treatments: before the start of cold acclimation (control point), at the start of acclimation, after one week of acclimation, after three weeks of acclimation, after freezing at -5 °C and freezing at -10 °C. A total of 11,125 differentially expressed genes (DEGs) were identified in the sensitive and 12,937 DEGs in the tolerant genotypes, when comparing the control vs. each of the acclimation and freezing treatments, as well as the end of acclimation vs. freezing treatments. Among the identified DEGs 3323 were unique to the sensitive genotypes, 5135 were unique to the tolerant genotypes and 7802 were shared. Genes upregulated during cold acclimation and freezing stress were linked to the MAPK signalling pathway, circadian rhythm, starch and sucrose metabolism, plant-pathogen interaction, carbon fixation, alpha-linoleic acid metabolism, carotenoid metabolism, glyoxylate and dicarboxylate metabolism pathways. Downregulated genes were linked to ATP-dependent chromatin remodelling, fatty acid elongation and DNA replication. The downregulation of fatty acid elongation and glutathione metabolism DEGs could indicate that the studied genotypes respond to cold stress in a novel or not yet well-characterized manner. |
| format | Article |
| id | doaj-art-bd5f9c4a90184330baa8dc694180b9f6 |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-bd5f9c4a90184330baa8dc694180b9f62024-12-19T11:01:54ZengElsevierPlant Stress2667-064X2024-12-0114100598Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing toleranceAkhil Reddy Pashapu0Gražina Statkevičiūtė1Ferenz Sustek-Sánchez2Mallikarjuna Rao Kovi3Odd Arne Rognli4Cecilia Sarmiento5Nils Rostoks6Kristina Jaškūnė7Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences (NMBU), Ås, NorwayLaboratory of Genetics and Physiology, Lithuanian Research Centre for Agriculture and Forestry, Institute of Agriculture, Akademija, LithuaniaDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, EstoniaDepartment of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences (NMBU), Ås, Norway; Corresponding authors.Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences (NMBU), Ås, NorwayDepartment of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, EstoniaDepartment of Microbiology and Biotechnology, Faculty of Medicine and Life Sciences, University of Latvia, Riga, LatviaLaboratory of Genetics and Physiology, Lithuanian Research Centre for Agriculture and Forestry, Institute of Agriculture, Akademija, Lithuania; Corresponding authors.Low freezing tolerance threatens the survival and productivity of perennial ryegrass under northern climate. In this study, we aimed to identify transcriptional changes in plants subjected to low and freezing temperatures as well as to elucidate differences between tolerant and sensitive genotypes. Response to freezing stress was evaluated in a panel of 160 perennial ryegrass genotypes by measuring electrolyte leakage after exposure to -12 °C and -14 °C for 24 h. Two tolerant and two sensitive genotypes were selected for the transcriptome analysis. Crown tissue samples were collected at six treatments: before the start of cold acclimation (control point), at the start of acclimation, after one week of acclimation, after three weeks of acclimation, after freezing at -5 °C and freezing at -10 °C. A total of 11,125 differentially expressed genes (DEGs) were identified in the sensitive and 12,937 DEGs in the tolerant genotypes, when comparing the control vs. each of the acclimation and freezing treatments, as well as the end of acclimation vs. freezing treatments. Among the identified DEGs 3323 were unique to the sensitive genotypes, 5135 were unique to the tolerant genotypes and 7802 were shared. Genes upregulated during cold acclimation and freezing stress were linked to the MAPK signalling pathway, circadian rhythm, starch and sucrose metabolism, plant-pathogen interaction, carbon fixation, alpha-linoleic acid metabolism, carotenoid metabolism, glyoxylate and dicarboxylate metabolism pathways. Downregulated genes were linked to ATP-dependent chromatin remodelling, fatty acid elongation and DNA replication. The downregulation of fatty acid elongation and glutathione metabolism DEGs could indicate that the studied genotypes respond to cold stress in a novel or not yet well-characterized manner.http://www.sciencedirect.com/science/article/pii/S2667064X24002513Lolium perenneElectrolyte leakageRNA-seqCold acclimationFreezing stressDifferentially expressed genes (DEGs) |
| spellingShingle | Akhil Reddy Pashapu Gražina Statkevičiūtė Ferenz Sustek-Sánchez Mallikarjuna Rao Kovi Odd Arne Rognli Cecilia Sarmiento Nils Rostoks Kristina Jaškūnė Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance Plant Stress Lolium perenne Electrolyte leakage RNA-seq Cold acclimation Freezing stress Differentially expressed genes (DEGs) |
| title | Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance |
| title_full | Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance |
| title_fullStr | Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance |
| title_full_unstemmed | Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance |
| title_short | Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance |
| title_sort | transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting freezing tolerance |
| topic | Lolium perenne Electrolyte leakage RNA-seq Cold acclimation Freezing stress Differentially expressed genes (DEGs) |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X24002513 |
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