Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research
Abstract Lyme disease is caused by an infection with the spirochete Borrelia burgdorferi, and is the most common vector-borne disease in North America. B. burgdorferi isolates harbor extensive genomic and proteomic variability and further comparison of isolates is key to understanding the infectivit...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-04047-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846137301769388032 |
|---|---|
| author | Panga J. Reddy Zhi Sun Helisa H. Wippel David H. Baxter Kristian Swearingen David D. Shteynberg Mukul K. Midha Melissa J. Caimano Klemen Strle Yongwook Choi Agnes P. Chan Nicholas J. Schork Andrea S. Varela-Stokes Robert L. Moritz |
| author_facet | Panga J. Reddy Zhi Sun Helisa H. Wippel David H. Baxter Kristian Swearingen David D. Shteynberg Mukul K. Midha Melissa J. Caimano Klemen Strle Yongwook Choi Agnes P. Chan Nicholas J. Schork Andrea S. Varela-Stokes Robert L. Moritz |
| author_sort | Panga J. Reddy |
| collection | DOAJ |
| description | Abstract Lyme disease is caused by an infection with the spirochete Borrelia burgdorferi, and is the most common vector-borne disease in North America. B. burgdorferi isolates harbor extensive genomic and proteomic variability and further comparison of isolates is key to understanding the infectivity of the spirochetes and biological impacts of identified sequence variants. Here, we applied both transcriptome analysis and mass spectrometry-based proteomics to assemble peptide datasets of B. burgdorferi laboratory isolates B31, MM1, and the infective isolate B31-5A4, to provide a publicly available Borrelia PeptideAtlas. Included are total proteome, secretome, and membrane proteome identifications of the individual isolates. Proteomic data collected from 35 different experiment datasets, totaling 386 mass spectrometry runs, have identified 81,967 distinct peptides, which map to 1,113 proteins. The Borrelia PeptideAtlas covers 86% of the total B31 proteome of 1,291 protein sequences. The Borrelia PeptideAtlas is an extensible comprehensive peptide repository with proteomic information from B. burgdorferi isolates useful for Lyme disease research. |
| format | Article |
| id | doaj-art-bd4e1e68f2674b299d11797f90ce48b7 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-bd4e1e68f2674b299d11797f90ce48b72024-12-08T12:18:01ZengNature PortfolioScientific Data2052-44632024-12-0111111710.1038/s41597-024-04047-9Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchPanga J. Reddy0Zhi Sun1Helisa H. Wippel2David H. Baxter3Kristian Swearingen4David D. Shteynberg5Mukul K. Midha6Melissa J. Caimano7Klemen Strle8Yongwook Choi9Agnes P. Chan10Nicholas J. Schork11Andrea S. Varela-Stokes12Robert L. Moritz13Institute for Systems BiologyInstitute for Systems BiologyInstitute for Systems BiologyInstitute for Systems BiologyInstitute for Systems BiologyInstitute for Systems BiologyInstitute for Systems BiologyDepartment of Medicine, UConn HealthDepartment of Molecular Biology and Microbiology, Tufts University School of MedicineTranslational Genomics Research InstituteTranslational Genomics Research InstituteTranslational Genomics Research InstituteTufts University Cummings School of Veterinary Medicine, Department of Comparative PathobiologyInstitute for Systems BiologyAbstract Lyme disease is caused by an infection with the spirochete Borrelia burgdorferi, and is the most common vector-borne disease in North America. B. burgdorferi isolates harbor extensive genomic and proteomic variability and further comparison of isolates is key to understanding the infectivity of the spirochetes and biological impacts of identified sequence variants. Here, we applied both transcriptome analysis and mass spectrometry-based proteomics to assemble peptide datasets of B. burgdorferi laboratory isolates B31, MM1, and the infective isolate B31-5A4, to provide a publicly available Borrelia PeptideAtlas. Included are total proteome, secretome, and membrane proteome identifications of the individual isolates. Proteomic data collected from 35 different experiment datasets, totaling 386 mass spectrometry runs, have identified 81,967 distinct peptides, which map to 1,113 proteins. The Borrelia PeptideAtlas covers 86% of the total B31 proteome of 1,291 protein sequences. The Borrelia PeptideAtlas is an extensible comprehensive peptide repository with proteomic information from B. burgdorferi isolates useful for Lyme disease research.https://doi.org/10.1038/s41597-024-04047-9 |
| spellingShingle | Panga J. Reddy Zhi Sun Helisa H. Wippel David H. Baxter Kristian Swearingen David D. Shteynberg Mukul K. Midha Melissa J. Caimano Klemen Strle Yongwook Choi Agnes P. Chan Nicholas J. Schork Andrea S. Varela-Stokes Robert L. Moritz Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research Scientific Data |
| title | Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research |
| title_full | Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research |
| title_fullStr | Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research |
| title_full_unstemmed | Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research |
| title_short | Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research |
| title_sort | borrelia peptideatlas a proteome resource of common borrelia burgdorferi isolates for lyme research |
| url | https://doi.org/10.1038/s41597-024-04047-9 |
| work_keys_str_mv | AT pangajreddy borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT zhisun borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT helisahwippel borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT davidhbaxter borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT kristianswearingen borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT daviddshteynberg borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT mukulkmidha borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT melissajcaimano borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT klemenstrle borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT yongwookchoi borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT agnespchan borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT nicholasjschork borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT andreasvarelastokes borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch AT robertlmoritz borreliapeptideatlasaproteomeresourceofcommonborreliaburgdorferiisolatesforlymeresearch |