Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice
Abstract Several lines of evidence implicate that chronic periodontitis (CP) increases the risk of mental illnesses, such as anxiety and depression, yet, the associated molecular mechanism for this remains poorly defined. Here, it is reported that mice subjected to CP exhibited depression‐like behav...
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| Format: | Article |
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Wiley
2024-12-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202406269 |
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| author | Ting Cao Dan Tian Si‐Ying Wang Yue Pan Zhi‐Xuan Xia Wei‐Kai Chen Shao‐Wei Yang Qing‐Quan Zeng Yue‐Ling Zhao Ling Zheng Ning Li Zhong‐Meng Lai Yi‐Xiao Luo Zu‐Cheng Shen |
| author_facet | Ting Cao Dan Tian Si‐Ying Wang Yue Pan Zhi‐Xuan Xia Wei‐Kai Chen Shao‐Wei Yang Qing‐Quan Zeng Yue‐Ling Zhao Ling Zheng Ning Li Zhong‐Meng Lai Yi‐Xiao Luo Zu‐Cheng Shen |
| author_sort | Ting Cao |
| collection | DOAJ |
| description | Abstract Several lines of evidence implicate that chronic periodontitis (CP) increases the risk of mental illnesses, such as anxiety and depression, yet, the associated molecular mechanism for this remains poorly defined. Here, it is reported that mice subjected to CP exhibited depression‐like behaviors and hippocampal memory deficits, accompanied by synapse loss and neurogenesis impairment in the hippocampus. RNA microarray analysis disclosed that albumin D‐site‐binding protein (DBP) is identified as the most prominently upregulated target gene following CP, and in vivo and in vitro immunofluorescence methods showed that DBP is preferentially expressed in microglia but not neurons or astrocytes in the hippocampus. Interestingly, it is found that the expression of DBP is significantly increased in microglia after CP, and knockdown of microglial DBP ameliorated the behavioral abnormality, as well as reversed the synapse loss and hippocampal neurogenesis damage induced by CP. Furthermore, DBP knockdown improved the CP‐induced hippocampal inflammation and microglial polarization. Collectively, these results indicate a critical role of DBP in orchestrating chronic periodontitis‐related behavioral abnormality, hippocampal synapse loss and neurogenesis deficits, in which the microglial activation may be indispensably involved. |
| format | Article |
| id | doaj-art-bcfa56d34f0a411a84c86ce2b5112688 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-bcfa56d34f0a411a84c86ce2b51126882024-12-11T16:00:49ZengWileyAdvanced Science2198-38442024-12-011146n/an/a10.1002/advs.202406269Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in MiceTing Cao0Dan Tian1Si‐Ying Wang2Yue Pan3Zhi‐Xuan Xia4Wei‐Kai Chen5Shao‐Wei Yang6Qing‐Quan Zeng7Yue‐Ling Zhao8Ling Zheng9Ning Li10Zhong‐Meng Lai11Yi‐Xiao Luo12Zu‐Cheng Shen13Department of Children's Stomatology Stomatological Hospital of Xiamen Medical College, Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment Xiamen 361003 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology, School of Basic Medicine and Life Science Hainan Medical University Haikou 571199 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaFujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaDepartment of Anesthesiology Union Hospital, Fujian Medical University Fuzhou 350001 ChinaHunan Province People's Hospital The First‐affiliated Hospital of Hunan Normal University Changsha 410002 ChinaDepartment of Pharmacology School of Pharmacy Fujian Medical University Fuzhou 350122 ChinaAbstract Several lines of evidence implicate that chronic periodontitis (CP) increases the risk of mental illnesses, such as anxiety and depression, yet, the associated molecular mechanism for this remains poorly defined. Here, it is reported that mice subjected to CP exhibited depression‐like behaviors and hippocampal memory deficits, accompanied by synapse loss and neurogenesis impairment in the hippocampus. RNA microarray analysis disclosed that albumin D‐site‐binding protein (DBP) is identified as the most prominently upregulated target gene following CP, and in vivo and in vitro immunofluorescence methods showed that DBP is preferentially expressed in microglia but not neurons or astrocytes in the hippocampus. Interestingly, it is found that the expression of DBP is significantly increased in microglia after CP, and knockdown of microglial DBP ameliorated the behavioral abnormality, as well as reversed the synapse loss and hippocampal neurogenesis damage induced by CP. Furthermore, DBP knockdown improved the CP‐induced hippocampal inflammation and microglial polarization. Collectively, these results indicate a critical role of DBP in orchestrating chronic periodontitis‐related behavioral abnormality, hippocampal synapse loss and neurogenesis deficits, in which the microglial activation may be indispensably involved.https://doi.org/10.1002/advs.202406269albumin D‐site‐binding proteinchronic periodontitisdepression‐like behaviorshippocampusmicroglia |
| spellingShingle | Ting Cao Dan Tian Si‐Ying Wang Yue Pan Zhi‐Xuan Xia Wei‐Kai Chen Shao‐Wei Yang Qing‐Quan Zeng Yue‐Ling Zhao Ling Zheng Ning Li Zhong‐Meng Lai Yi‐Xiao Luo Zu‐Cheng Shen Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice Advanced Science albumin D‐site‐binding protein chronic periodontitis depression‐like behaviors hippocampus microglia |
| title | Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice |
| title_full | Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice |
| title_fullStr | Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice |
| title_full_unstemmed | Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice |
| title_short | Microglial DBP Signaling Mediates Behavioral Abnormality Induced by Chronic Periodontitis in Mice |
| title_sort | microglial dbp signaling mediates behavioral abnormality induced by chronic periodontitis in mice |
| topic | albumin D‐site‐binding protein chronic periodontitis depression‐like behaviors hippocampus microglia |
| url | https://doi.org/10.1002/advs.202406269 |
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