Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis
Abstract The high fat diet alters intestinal microbiota due to increased intestinal permeability and susceptibility to microbial antigens leads to metabolic endotoxemia. But probiotic juices reported for various health benefits. In this background we hypothesized that pectinase treated probiotic ban...
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Nature Portfolio
2020-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-73670-4 |
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| author | Prabhakar Yellanur Konda Vijayakumar Poondla Krishna Kumar Jaiswal Sreenivasulu Dasari Reddemma Uyyala Venkata Prasad Surtineni Janardhan Yadav Egi Anthony Johnson Antony Masilamani Lakshmi Bestha Sreenath Konanki Muthukumaran Muthulingam Lakshman Kumar Lingamgunta Bindu Prasuna Aloor Sridevi Tirumalaraju Ankanna Sade Venkata Ratnam Kamsala Sreeharsha Nagaraja Ranjani Ramakrishnan Vijayakumar Natesan |
| author_facet | Prabhakar Yellanur Konda Vijayakumar Poondla Krishna Kumar Jaiswal Sreenivasulu Dasari Reddemma Uyyala Venkata Prasad Surtineni Janardhan Yadav Egi Anthony Johnson Antony Masilamani Lakshmi Bestha Sreenath Konanki Muthukumaran Muthulingam Lakshman Kumar Lingamgunta Bindu Prasuna Aloor Sridevi Tirumalaraju Ankanna Sade Venkata Ratnam Kamsala Sreeharsha Nagaraja Ranjani Ramakrishnan Vijayakumar Natesan |
| author_sort | Prabhakar Yellanur Konda |
| collection | DOAJ |
| description | Abstract The high fat diet alters intestinal microbiota due to increased intestinal permeability and susceptibility to microbial antigens leads to metabolic endotoxemia. But probiotic juices reported for various health benefits. In this background we hypothesized that pectinase treated probiotic banana juice has diverse effects on HFD induced obesity and non-alcoholic steatohepatitis. 20 weeks fed HFD successfully induced obesity and its associated complications in experimental rats. The supplementation of probiotic banana juice for 5 months at a dose of 5 mL/kg bw/day resulted significant decrease (p < 0.05) in body weight (380 ± 0.34), total fat (72 ± 0.8), fat percentage (17 ± 0.07) and fat free mass (165 ± 0.02). Reduction (p < 0.05) in insulin resistance (5.20 ± 0.03), lipid profile (TC 120 ± 0.05; TG 160 ± 0.24; HDL 38 ± 0.03), liver lipid peroxidation (0.7 ± 0.01), hepatic enzyme markers (AST 82 ± 0.06; ALT 78 ± 0.34; ALP 42 ± 0.22), and hepatic steatosis by increasing liver antioxidant potential (CAT 1.4 ± 0.30; GSH 1.04 ± 0.04; SOD 0.82 ± 0.22) with normal hepatic triglycerides (15 ± 0.02) and glycogen (0.022 ± 0.15) contents and also showed normal liver size, less accumulation of lipid droplets with only a few congestion. It is concluded that the increased intestinal S. cerevisiae yeast can switch anti-obesity, antidiabetic, antioxidative stress, antioxidant and anti-hepatosteatosis effect. This study results will have significant implications for treatment of NAFLD. |
| format | Article |
| id | doaj-art-bcb99e63b6b4489691f3e6d933cf6b90 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-bcb99e63b6b4489691f3e6d933cf6b902025-01-12T12:25:23ZengNature PortfolioScientific Reports2045-23222020-10-0110111710.1038/s41598-020-73670-4Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosisPrabhakar Yellanur Konda0Vijayakumar Poondla1Krishna Kumar Jaiswal2Sreenivasulu Dasari3Reddemma Uyyala4Venkata Prasad Surtineni5Janardhan Yadav Egi6Anthony Johnson Antony Masilamani7Lakshmi Bestha8Sreenath Konanki9Muthukumaran Muthulingam10Lakshman Kumar Lingamgunta11Bindu Prasuna Aloor12Sridevi Tirumalaraju13Ankanna Sade14Venkata Ratnam Kamsala15Sreeharsha Nagaraja16Ranjani Ramakrishnan17Vijayakumar Natesan18Department of Biochemistry, Krijan BiotechDepartment of Chemistry, Indian Institute of Technology HyderabadDepartment of Chemistry, Uttaranchal UniversityDepartment of Biochemistry, Sri Venkateswara UniversityDepartment of Biochemistry, Krijan BiotechDepartment of Biochemistry, Sri Venkateswara UniversityDepartment of Biotechnology, Sri Venkateswara UniversityDepartment of Botany, Sri Krishnadevaraya UniversityDepartment of Zoology, Sri Krishnadevaraya UniversityDepartment of Biotechnology, Sri Krishnadevaraya UniversityDepartment of Ecology and Environmental Sciences, Pondicherry UniversityDepartment of Biochemistry, Sri Venkateswara UniversityDepartment of Botany, Rayalaseema UniversityMahatma Gandhi National Institute of Research and Social ActionDepartment of Botany, Sri Venkateswara UniversityDepartment of Botany, Rayalaseema UniversityDepartment of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal UniversityDepartment of Virology, Sri Venkateswara UniversityDepartment of Biochemistry and Biotechnology, Annamalai UniversityAbstract The high fat diet alters intestinal microbiota due to increased intestinal permeability and susceptibility to microbial antigens leads to metabolic endotoxemia. But probiotic juices reported for various health benefits. In this background we hypothesized that pectinase treated probiotic banana juice has diverse effects on HFD induced obesity and non-alcoholic steatohepatitis. 20 weeks fed HFD successfully induced obesity and its associated complications in experimental rats. The supplementation of probiotic banana juice for 5 months at a dose of 5 mL/kg bw/day resulted significant decrease (p < 0.05) in body weight (380 ± 0.34), total fat (72 ± 0.8), fat percentage (17 ± 0.07) and fat free mass (165 ± 0.02). Reduction (p < 0.05) in insulin resistance (5.20 ± 0.03), lipid profile (TC 120 ± 0.05; TG 160 ± 0.24; HDL 38 ± 0.03), liver lipid peroxidation (0.7 ± 0.01), hepatic enzyme markers (AST 82 ± 0.06; ALT 78 ± 0.34; ALP 42 ± 0.22), and hepatic steatosis by increasing liver antioxidant potential (CAT 1.4 ± 0.30; GSH 1.04 ± 0.04; SOD 0.82 ± 0.22) with normal hepatic triglycerides (15 ± 0.02) and glycogen (0.022 ± 0.15) contents and also showed normal liver size, less accumulation of lipid droplets with only a few congestion. It is concluded that the increased intestinal S. cerevisiae yeast can switch anti-obesity, antidiabetic, antioxidative stress, antioxidant and anti-hepatosteatosis effect. This study results will have significant implications for treatment of NAFLD.https://doi.org/10.1038/s41598-020-73670-4 |
| spellingShingle | Prabhakar Yellanur Konda Vijayakumar Poondla Krishna Kumar Jaiswal Sreenivasulu Dasari Reddemma Uyyala Venkata Prasad Surtineni Janardhan Yadav Egi Anthony Johnson Antony Masilamani Lakshmi Bestha Sreenath Konanki Muthukumaran Muthulingam Lakshman Kumar Lingamgunta Bindu Prasuna Aloor Sridevi Tirumalaraju Ankanna Sade Venkata Ratnam Kamsala Sreeharsha Nagaraja Ranjani Ramakrishnan Vijayakumar Natesan Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis Scientific Reports |
| title | Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis |
| title_full | Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis |
| title_fullStr | Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis |
| title_full_unstemmed | Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis |
| title_short | Pathophysiology of high fat diet induced obesity: impact of probiotic banana juice on obesity associated complications and hepatosteatosis |
| title_sort | pathophysiology of high fat diet induced obesity impact of probiotic banana juice on obesity associated complications and hepatosteatosis |
| url | https://doi.org/10.1038/s41598-020-73670-4 |
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