Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek)
Abstract The mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) causes Yellow Mosaic Disease (YMD) in mungbean (Vigna radiata L.). The biochemical assays including total phenol content (TPC), total flavonoid content (TFC), ascorbic acid (AA), DPPH (2,2-diphenyl-1-picrylhyd...
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Nature Portfolio
2024-12-01
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| author | Manju Kohli Hina Bansal Muraleedhar Aski Gyan P. Mishra B. R. Shashidhar Anirban Roy Soma Gupta Subodh K. Sinha Brijesh Kumar Mishra Nikki Kumari Atul Kumar Ranjeet Ranjan Kumar Ramakrishnan M. Nair Harsh Kumar Dikshit |
| author_facet | Manju Kohli Hina Bansal Muraleedhar Aski Gyan P. Mishra B. R. Shashidhar Anirban Roy Soma Gupta Subodh K. Sinha Brijesh Kumar Mishra Nikki Kumari Atul Kumar Ranjeet Ranjan Kumar Ramakrishnan M. Nair Harsh Kumar Dikshit |
| author_sort | Manju Kohli |
| collection | DOAJ |
| description | Abstract The mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) causes Yellow Mosaic Disease (YMD) in mungbean (Vigna radiata L.). The biochemical assays including total phenol content (TPC), total flavonoid content (TFC), ascorbic acid (AA), DPPH (2,2-diphenyl-1-picrylhydrazyl), and FRAP (Ferric Reducing Antioxidant Power) were used to study the mungbean plants defense response to MYMIV infection. A wide range was recorded for the Area Under Disease Progress Curve (AUDPC; 1.75-1266.98) and coefficient of infection (CI; 0.33–45.53). In YMD susceptible genotypes, significant variations were observed for TPC [2001.27-2834.13 mgGAE/100 g dry weight (DW)], TFC (252.65–341.30 mg/100 g DW), AA (40.33–64.69 mg/100 g DW), DPPH (32.11–53.47% scavenging effect DW), and FRAP (48.99-101.22 µmol Fe2+/g DW). Similarly, in resistant genotypes also wide range was recorded for TPC (1788.50-2286.38 mgGAE/100 g DW), TFC (206.12–337.32 mg/100 gDAS samples varied from 384. 6.46–47.64% scavenging effect DW), and FRAP (53.68-114.24 µmol Fe2+/g DW). Except for FRAP, other studied parameters were in the lower range in the resistant genotypes than the susceptible genotypes. Genome-wide association studies (GWAS) of 132 genotypes have identified 31,953 single nucleotide polymorphism (SNPs). MLM (Mixed Linear Model) and BLINK (Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway) models have identified 119 shared SNPs for various biochemical traits and MYMIV resistance. The key candidate genes include VRADI09G06940 (YMD resistance, TIR-NBS-LRR class, chr. 9), VRADI01G05030 [flavonoid biosynthesis; MYB65 transcription factor (TF); chr. 1], VRADI03G07600 (phenol biosynthesis; GATA TF 16; chr. 3), VRADI04G08470 (ascorbic acid; heat shock protein 70 kDa protein; chr. 4), VRADI04G07510 (FRAP; subtilisin-like protease SBT1.9; chr. 4), and VRADI05G02870 (DPPH; vacuolar protein sorting-associated protein 2; chr. 5). The identified genomic resources will enhance mungbean genomics and facilitate the advancement of genomic-assisted breeding in mungbean. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Scientific Reports |
| spelling | doaj-art-bcaba38a59ef4aa2a2a8687fa906ed322025-01-05T12:26:34ZengNature PortfolioScientific Reports2045-23222024-12-0114112110.1038/s41598-024-82836-3Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek)Manju Kohli0Hina Bansal1Muraleedhar Aski2Gyan P. Mishra3B. R. Shashidhar4Anirban Roy5Soma Gupta6Subodh K. Sinha7Brijesh Kumar Mishra8Nikki Kumari9Atul Kumar10Ranjeet Ranjan Kumar11Ramakrishnan M. Nair12Harsh Kumar Dikshit13Division of Genetics, Indian Agricultural Research InstituteCenter for Computational Biology and Bioinformatics, Amity UniversityDivision of Genetics, Indian Agricultural Research InstituteDivision of Genetics, Indian Agricultural Research InstituteDivision of Genetics, Indian Agricultural Research InstituteDivision of Plant Pathology, Indian Agricultural Research InstituteDivision of Genetics, Indian Agricultural Research InstituteNational Institute for Plant BiotechnologyDivision of Microbiology, Indian Agricultural Research InstituteDivision of Genetics, Indian Agricultural Research InstituteDivision of Seed Science and Technology, Indian Agricultural Research InstituteDivision of Biochemistry, Indian Agricultural Research InstituteWorld Vegetable Center, South Asia, ICRISAT Campus PatancheruDivision of Genetics, Indian Agricultural Research InstituteAbstract The mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) causes Yellow Mosaic Disease (YMD) in mungbean (Vigna radiata L.). The biochemical assays including total phenol content (TPC), total flavonoid content (TFC), ascorbic acid (AA), DPPH (2,2-diphenyl-1-picrylhydrazyl), and FRAP (Ferric Reducing Antioxidant Power) were used to study the mungbean plants defense response to MYMIV infection. A wide range was recorded for the Area Under Disease Progress Curve (AUDPC; 1.75-1266.98) and coefficient of infection (CI; 0.33–45.53). In YMD susceptible genotypes, significant variations were observed for TPC [2001.27-2834.13 mgGAE/100 g dry weight (DW)], TFC (252.65–341.30 mg/100 g DW), AA (40.33–64.69 mg/100 g DW), DPPH (32.11–53.47% scavenging effect DW), and FRAP (48.99-101.22 µmol Fe2+/g DW). Similarly, in resistant genotypes also wide range was recorded for TPC (1788.50-2286.38 mgGAE/100 g DW), TFC (206.12–337.32 mg/100 gDAS samples varied from 384. 6.46–47.64% scavenging effect DW), and FRAP (53.68-114.24 µmol Fe2+/g DW). Except for FRAP, other studied parameters were in the lower range in the resistant genotypes than the susceptible genotypes. Genome-wide association studies (GWAS) of 132 genotypes have identified 31,953 single nucleotide polymorphism (SNPs). MLM (Mixed Linear Model) and BLINK (Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway) models have identified 119 shared SNPs for various biochemical traits and MYMIV resistance. The key candidate genes include VRADI09G06940 (YMD resistance, TIR-NBS-LRR class, chr. 9), VRADI01G05030 [flavonoid biosynthesis; MYB65 transcription factor (TF); chr. 1], VRADI03G07600 (phenol biosynthesis; GATA TF 16; chr. 3), VRADI04G08470 (ascorbic acid; heat shock protein 70 kDa protein; chr. 4), VRADI04G07510 (FRAP; subtilisin-like protease SBT1.9; chr. 4), and VRADI05G02870 (DPPH; vacuolar protein sorting-associated protein 2; chr. 5). The identified genomic resources will enhance mungbean genomics and facilitate the advancement of genomic-assisted breeding in mungbean.https://doi.org/10.1038/s41598-024-82836-3Antioxidant activityYellow mosaic diseaseGWASGreen gramSNPs |
| spellingShingle | Manju Kohli Hina Bansal Muraleedhar Aski Gyan P. Mishra B. R. Shashidhar Anirban Roy Soma Gupta Subodh K. Sinha Brijesh Kumar Mishra Nikki Kumari Atul Kumar Ranjeet Ranjan Kumar Ramakrishnan M. Nair Harsh Kumar Dikshit Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) Scientific Reports Antioxidant activity Yellow mosaic disease GWAS Green gram SNPs |
| title | Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) |
| title_full | Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) |
| title_fullStr | Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) |
| title_full_unstemmed | Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) |
| title_short | Genome-wide association mapping of biochemical traits and its correlation with MYMIV resistance in mungbean (Vigna radiata L. Wilczek) |
| title_sort | genome wide association mapping of biochemical traits and its correlation with mymiv resistance in mungbean vigna radiata l wilczek |
| topic | Antioxidant activity Yellow mosaic disease GWAS Green gram SNPs |
| url | https://doi.org/10.1038/s41598-024-82836-3 |
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