Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High‐Cryotolerant Rams or Extender
ABSTRACT Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high‐cryotolerant rams or extender on semen quality parameters in low‐cryotolerant rams. Rams previously determined as low (n = 6...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-05-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70381 |
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| Summary: | ABSTRACT Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high‐cryotolerant rams or extender on semen quality parameters in low‐cryotolerant rams. Rams previously determined as low (n = 6) and high‐cryotolerant (n = 5) were used in the breeding season. High‐cryotolerant rams were only included with their SP in the SP+ group. Each normospermic fresh ejaculate from low‐cryotolerant rams was split into three aliquots to form the groups. The control aliquot was diluted using a two‐step process. Centrifugation removed SP from the second aliquot (SP− group), and the sperm pellet was rediluted with a Tris‐based extender using the same amount of SP removed. The third aliquot's SP was removed as in the SP− group, but the remaining sperm pellet was rediluted with fresh SP harvested from high‐cryotolerant rams using the same amounts of removed SP in the SP+ group. Semen was rediluted with a Tris‐based extender in the SP− and SP+ groups using a two‐step process as in the control group. Sperm quality was similar between the groups after dilution and equilibration. However, the post‐thaw semen quality was higher in the SP− (one parameter) and SP+ (four parameters) groups than in the control group (p < 0.05). Additionally, these parameters were similar in the SP− and SP+ groups. This study showed that, although an adequate extender had positive effects, replacing the whole SP with that of high‐cryotolerant rams may better solve the low cryotolerance problem in ram semen. |
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| ISSN: | 2053-1095 |