Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics

Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics

Summary: Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain are now available. However, few effective approaches enable both optical and mechanical access to the brain. Here, we present a protocol for synthesizing, implanting, and using polydimet...

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Bibliographic Details
Main Authors: Kengo Takahashi, Gerjan Huis in ‘t Veld, Davide Benedetti, Jun-Ying Wang, Samuel Pontes Quero, Rafael Yuste, Cyriel M.A. Pennartz, Umberto Olcese
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Microscopy
Neuroscience
Cognitive Neuroscience
Behavior
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003703
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