A Transcriptome Approach Evaluating the Effects of Atractylenolide I on the Secretion of Estradiol and Progesterone in Feline Ovarian Granulosa Cells
<i>Atractylodes macrocephala Koidz</i> (AMK) as an oriental medicine has been used in the treatment of threatened abortion. <i>Atractylenolide I</i> (AT-I) is one of the major bioactive components of AMK. This study aimed to investigate the effect of AT-I on the secretion of...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/11/12/663 |
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| Summary: | <i>Atractylodes macrocephala Koidz</i> (AMK) as an oriental medicine has been used in the treatment of threatened abortion. <i>Atractylenolide I</i> (AT-I) is one of the major bioactive components of AMK. This study aimed to investigate the effect of AT-I on the secretion of estradiol (E<sub>2</sub>) and progesterone (P<sub>4</sub>) in feline ovarian granulosa cells (FOGCs), which is necessary for pregnancy. At first, the proliferation of FOGCs after AT-I treatment was measured by CCK-8. Then, the synthesis of E<sub>2</sub> and P<sub>4</sub> were measured by ELISA. Lastly, transcriptome sequencing was used to detect the DEGs in the FOGCs, and RNA-seq results were verified by RT-qPCR and biochemical verification. It was found that AT-I could promote proliferation and the secretion of E<sub>2</sub> and P<sub>4</sub> in FOGCs; after AT-I treatment, 137 significant DEGs were observed, out of which 49 were up-regulated and 88 down-regulated. The DEGs revealed significant enrichment of 52 GO terms throughout the differentiation process (<i>p</i> < 0.05), as deciphered by Gene Ontology enrichment analysis. Kyoto Encyclopedia of Genes and Genomes analysis manifested that the DEGs were successfully annotated as members of 155 pathways, with 23 significantly enriched (<i>p</i> < 0.05). A relatively high number of genes were enriched for the cholesterol metabolism, ovarian steroidogenesis, and biosynthesis of unsaturated fatty acids. Furthermore, the contents of the total cholesterol and low-density lipoprotein cholesterol were decreased by AT-I treatment in the cell culture supernatant. The results indicated that AT-I could increase the ability of FOGCs to secrete E<sub>2</sub> and P<sub>4</sub>, which might be achieved by activation of cholesterol metabolism. |
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| ISSN: | 2306-7381 |