Dry resist laser patterning of flexible neural probes with fluidic functionality

Optogenetics enables insights into the development of neural diseases. Custom-designed neural probes are necessary to access targeted brain regions. Multifunctional and mechanically flexible probes with minimal footprint, comprising fluidic channels for drug delivery, light sources for optical stimu...

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Main Authors: Bucherer Andreas, Klein Eric, Paul Oliver, Ruther Patrick
Format: Article
Language:English
Published: De Gruyter 2024-12-01
Series:Current Directions in Biomedical Engineering
Subjects:
Online Access:https://doi.org/10.1515/cdbme-2024-2029
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author Bucherer Andreas
Klein Eric
Paul Oliver
Ruther Patrick
author_facet Bucherer Andreas
Klein Eric
Paul Oliver
Ruther Patrick
author_sort Bucherer Andreas
collection DOAJ
description Optogenetics enables insights into the development of neural diseases. Custom-designed neural probes are necessary to access targeted brain regions. Multifunctional and mechanically flexible probes with minimal footprint, comprising fluidic channels for drug delivery, light sources for optical stimulation, and micro electrodes for electro-physiological readout are beneficial due to reduced tissue trauma and consequently increased long-term stability. This study introduces a mechanically compliant neural probe that provides microfluidic channels and electrodes arranged close to fluidic outlet ports. The key challenge is to open the fluidic inlet and outlet ports avoiding potential channel clogging. A novel laserbased patterning process is demonstrated using a 248 nm KrF excimer laser. It offers high design flexibility in positioning the fluidic ports along the fluidic channels, as well their contamination-free opening under dry conditions. The laser patterning process applies reflective metal layers inside the fluidic channels, confining the laser ablation to the channel cover, thus minimizing potential damage of the channel substrate. Based on its high reflectivity and correspondingly low absorption and transmission at the applied laser wavelength, aluminium is determined to be the best choice for this protective layer.
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series Current Directions in Biomedical Engineering
spelling doaj-art-bc612f2f95124dc4a93ff99724a1a30c2025-01-02T05:56:32ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042024-12-0110411912210.1515/cdbme-2024-2029Dry resist laser patterning of flexible neural probes with fluidic functionalityBucherer Andreas0Klein Eric1Paul Oliver2Ruther Patrick3Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Kohler-Allee 103, 79110Freiburg, GermanyIMTEK and BrainLinks-BrainTools Center, University of Freiburg,Freiburg, GermanyIMTEK and BrainLinks-BrainTools Center, University of Freiburg,Freiburg, GermanyIMTEK and BrainLinks-BrainTools Center, University of Freiburg,Freiburg, GermanyOptogenetics enables insights into the development of neural diseases. Custom-designed neural probes are necessary to access targeted brain regions. Multifunctional and mechanically flexible probes with minimal footprint, comprising fluidic channels for drug delivery, light sources for optical stimulation, and micro electrodes for electro-physiological readout are beneficial due to reduced tissue trauma and consequently increased long-term stability. This study introduces a mechanically compliant neural probe that provides microfluidic channels and electrodes arranged close to fluidic outlet ports. The key challenge is to open the fluidic inlet and outlet ports avoiding potential channel clogging. A novel laserbased patterning process is demonstrated using a 248 nm KrF excimer laser. It offers high design flexibility in positioning the fluidic ports along the fluidic channels, as well their contamination-free opening under dry conditions. The laser patterning process applies reflective metal layers inside the fluidic channels, confining the laser ablation to the channel cover, thus minimizing potential damage of the channel substrate. Based on its high reflectivity and correspondingly low absorption and transmission at the applied laser wavelength, aluminium is determined to be the best choice for this protective layer.https://doi.org/10.1515/cdbme-2024-2029optogeneticsfluidic probelaser patterningdry resist
spellingShingle Bucherer Andreas
Klein Eric
Paul Oliver
Ruther Patrick
Dry resist laser patterning of flexible neural probes with fluidic functionality
Current Directions in Biomedical Engineering
optogenetics
fluidic probe
laser patterning
dry resist
title Dry resist laser patterning of flexible neural probes with fluidic functionality
title_full Dry resist laser patterning of flexible neural probes with fluidic functionality
title_fullStr Dry resist laser patterning of flexible neural probes with fluidic functionality
title_full_unstemmed Dry resist laser patterning of flexible neural probes with fluidic functionality
title_short Dry resist laser patterning of flexible neural probes with fluidic functionality
title_sort dry resist laser patterning of flexible neural probes with fluidic functionality
topic optogenetics
fluidic probe
laser patterning
dry resist
url https://doi.org/10.1515/cdbme-2024-2029
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AT kleineric dryresistlaserpatterningofflexibleneuralprobeswithfluidicfunctionality
AT pauloliver dryresistlaserpatterningofflexibleneuralprobeswithfluidicfunctionality
AT rutherpatrick dryresistlaserpatterningofflexibleneuralprobeswithfluidicfunctionality