TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion
Abstract Background Epithelial-mesenchymal transition (EMT) plays a crucial role in the migration and invasion capabilities of glioblastoma (GBM) cells. Several studies have established tubulin as a significant regulator of the EMT process. Tubulin beta 2B class IIb (TUBB2B), a critical component of...
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BMC
2024-12-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-024-03618-5 |
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| author | Junxi Li Zhengjun Zhou Junrong Zhang Ming Wang Xingzhao Luan Mingkuan Zhao Geng Jiang Guiyuan Wang Shenjie Li Wei Xiang Ligang Chen Jie Zhou |
| author_facet | Junxi Li Zhengjun Zhou Junrong Zhang Ming Wang Xingzhao Luan Mingkuan Zhao Geng Jiang Guiyuan Wang Shenjie Li Wei Xiang Ligang Chen Jie Zhou |
| author_sort | Junxi Li |
| collection | DOAJ |
| description | Abstract Background Epithelial-mesenchymal transition (EMT) plays a crucial role in the migration and invasion capabilities of glioblastoma (GBM) cells. Several studies have established tubulin as a significant regulator of the EMT process. Tubulin beta 2B class IIb (TUBB2B), a critical component of microtubules, has been linked to the prognosis of various tumors. However, the specific biological function and mechanism of TUBB2B in GBM remain unclear. Methods In vitro experiments demonstrated that TUBB2B knockdown inhibited the migration and invasion of GBM cells, while its overexpression enhanced these capabilities. Western blot, immunofluorescence (IF) and co-immunoprecipitation (Co-IP) assays revealed that TUBB2B interacts with Vimentin. Molecular docking and residue mutation scanning indicated that TUBB2B interacts with Vimentin at the R391/K392/A393/F394 sites. In vivo experiments using nude mice confirmed that TUBB2B knockdown inhibited GBM cell invasion and migration. Results TUBB2B was upregulated in GBM tissue samples compared with normal tissues. The sites of TUBB2B(R391/K392/A393/F394) physically interacts with Vimentin to induce EMT, which promotes migration and invasion. Conclusion TUBB2B may regulate EMT and promote the migration and invasion of GBM cells through its interaction with Vimentin, highlighting TUBB2B as a potential therapeutic target for GBM. |
| format | Article |
| id | doaj-art-bc2041b40e8d428e8dfab5de67fe336b |
| institution | Kabale University |
| issn | 1475-2867 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj-art-bc2041b40e8d428e8dfab5de67fe336b2024-12-22T12:49:07ZengBMCCancer Cell International1475-28672024-12-0124111410.1186/s12935-024-03618-5TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasionJunxi Li0Zhengjun Zhou1Junrong Zhang2Ming Wang3Xingzhao Luan4Mingkuan Zhao5Geng Jiang6Guiyuan Wang7Shenjie Li8Wei Xiang9Ligang Chen10Jie Zhou11Department of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityDepartment of Neurosurgery, The Affiliated Hospital, Southwest Medical UniversityAbstract Background Epithelial-mesenchymal transition (EMT) plays a crucial role in the migration and invasion capabilities of glioblastoma (GBM) cells. Several studies have established tubulin as a significant regulator of the EMT process. Tubulin beta 2B class IIb (TUBB2B), a critical component of microtubules, has been linked to the prognosis of various tumors. However, the specific biological function and mechanism of TUBB2B in GBM remain unclear. Methods In vitro experiments demonstrated that TUBB2B knockdown inhibited the migration and invasion of GBM cells, while its overexpression enhanced these capabilities. Western blot, immunofluorescence (IF) and co-immunoprecipitation (Co-IP) assays revealed that TUBB2B interacts with Vimentin. Molecular docking and residue mutation scanning indicated that TUBB2B interacts with Vimentin at the R391/K392/A393/F394 sites. In vivo experiments using nude mice confirmed that TUBB2B knockdown inhibited GBM cell invasion and migration. Results TUBB2B was upregulated in GBM tissue samples compared with normal tissues. The sites of TUBB2B(R391/K392/A393/F394) physically interacts with Vimentin to induce EMT, which promotes migration and invasion. Conclusion TUBB2B may regulate EMT and promote the migration and invasion of GBM cells through its interaction with Vimentin, highlighting TUBB2B as a potential therapeutic target for GBM.https://doi.org/10.1186/s12935-024-03618-5TUBB2BEpithelial-mesenchymal transitionGlioblastomaVimentin |
| spellingShingle | Junxi Li Zhengjun Zhou Junrong Zhang Ming Wang Xingzhao Luan Mingkuan Zhao Geng Jiang Guiyuan Wang Shenjie Li Wei Xiang Ligang Chen Jie Zhou TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion Cancer Cell International TUBB2B Epithelial-mesenchymal transition Glioblastoma Vimentin |
| title | TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion |
| title_full | TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion |
| title_fullStr | TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion |
| title_full_unstemmed | TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion |
| title_short | TUBB2B regulates epithelial-mesenchymal transition via interaction with Vimentin to promote glioma migration and invasion |
| title_sort | tubb2b regulates epithelial mesenchymal transition via interaction with vimentin to promote glioma migration and invasion |
| topic | TUBB2B Epithelial-mesenchymal transition Glioblastoma Vimentin |
| url | https://doi.org/10.1186/s12935-024-03618-5 |
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