Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles

Abstract Background Dorper sheep are celebrated for their fast maturation and superior meat quality, with some shedding their wool each spring. Wool shedding occurs naturally due to the hair follicle (HF) cycle, but its regulatory mechanisms remain unclear and need further investigation. Results In...

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Main Authors: Ningyue Zhang, Yifan Wang, Jiankui Wang, Liang Zhang, Haoran Sun, Xiaochun Yuan, Siyu Wang, Chunguang Wang, Xinhai LI
Format: Article
Language:English
Published: BMC 2024-12-01
Series:BMC Genomics
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Online Access:https://doi.org/10.1186/s12864-024-11059-7
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author Ningyue Zhang
Yifan Wang
Jiankui Wang
Liang Zhang
Haoran Sun
Xiaochun Yuan
Siyu Wang
Chunguang Wang
Xinhai LI
author_facet Ningyue Zhang
Yifan Wang
Jiankui Wang
Liang Zhang
Haoran Sun
Xiaochun Yuan
Siyu Wang
Chunguang Wang
Xinhai LI
author_sort Ningyue Zhang
collection DOAJ
description Abstract Background Dorper sheep are celebrated for their fast maturation and superior meat quality, with some shedding their wool each spring. Wool shedding occurs naturally due to the hair follicle (HF) cycle, but its regulatory mechanisms remain unclear and need further investigation. Results In this study, shedding and non-shedding sheep were selected from the same Dorper flock. Skin samples were collected in September of the first year and January and March of the following years. RNA sequencing was performed on these samples. Principal component analysis (PCA) was used to assess the results. A total of 2536 differentially expressed genes (DEGs) were identified. Using a clustering heatmap and fuzzy clustering analysis three distinct gene expression patterns were identified: A pattern (high expression in anagen), T1 pattern, and T2 pattern (high expression in telogen). For each pattern, differentially expressed genes (DEGs) were analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Combining this with pathway expression analysis, six A-pattern and fourteen T-pattern pathways linked to telogen-anagen transition in the HF cycle were identified. Networks of key pathways were then constructed. Additionally, key genes were identified in the telogen-anagen transition, including one A-pattern gene and seven T-pattern (T1, 1; T2, 6) genes, using the Maximal Clique Centrality (MCC) tool in Cytoscape. Predicted transcription factors (TFs) involved in key pathways, such as LEF and STAT5B, were identified. Finally, RNA-seq results were confirmed by RT-qPCR. Conclusion This study highlights critical genes and pathways in the telogen-anagen transition, and transcriptome sequencing along with bioinformatics analysis provides new insights into the regulatory mechanisms of the HF cycle and development.
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spelling doaj-art-bc111c66aadb4eac9639a1d8f7eef93b2024-12-08T12:19:34ZengBMCBMC Genomics1471-21642024-12-0125111410.1186/s12864-024-11059-7Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair folliclesNingyue Zhang0Yifan Wang1Jiankui Wang2Liang Zhang3Haoran Sun4Xiaochun Yuan5Siyu Wang6Chunguang Wang7Xinhai LI8College of Animal Science and Technology, Ningxia UniversityCollege of Animal Science and Technology, Ningxia UniversityBeijing Key Laboratory of Animal Genetic Improvement, China Agricultural UniversityChina Animal Husbandry Group CNCollege of Animal Science and Technology, Ningxia UniversityCollege of Animal Science and Technology, Ningxia UniversityCollege of Animal Science and Technology, Ningxia UniversityCollege of Animal Science and Technology, Ningxia UniversityCollege of Animal Science and Technology, Ningxia UniversityAbstract Background Dorper sheep are celebrated for their fast maturation and superior meat quality, with some shedding their wool each spring. Wool shedding occurs naturally due to the hair follicle (HF) cycle, but its regulatory mechanisms remain unclear and need further investigation. Results In this study, shedding and non-shedding sheep were selected from the same Dorper flock. Skin samples were collected in September of the first year and January and March of the following years. RNA sequencing was performed on these samples. Principal component analysis (PCA) was used to assess the results. A total of 2536 differentially expressed genes (DEGs) were identified. Using a clustering heatmap and fuzzy clustering analysis three distinct gene expression patterns were identified: A pattern (high expression in anagen), T1 pattern, and T2 pattern (high expression in telogen). For each pattern, differentially expressed genes (DEGs) were analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Combining this with pathway expression analysis, six A-pattern and fourteen T-pattern pathways linked to telogen-anagen transition in the HF cycle were identified. Networks of key pathways were then constructed. Additionally, key genes were identified in the telogen-anagen transition, including one A-pattern gene and seven T-pattern (T1, 1; T2, 6) genes, using the Maximal Clique Centrality (MCC) tool in Cytoscape. Predicted transcription factors (TFs) involved in key pathways, such as LEF and STAT5B, were identified. Finally, RNA-seq results were confirmed by RT-qPCR. Conclusion This study highlights critical genes and pathways in the telogen-anagen transition, and transcriptome sequencing along with bioinformatics analysis provides new insights into the regulatory mechanisms of the HF cycle and development.https://doi.org/10.1186/s12864-024-11059-7Dorper sheepSheddingHair cycleRNA-seqHair follicleTranscriptome analysis
spellingShingle Ningyue Zhang
Yifan Wang
Jiankui Wang
Liang Zhang
Haoran Sun
Xiaochun Yuan
Siyu Wang
Chunguang Wang
Xinhai LI
Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles
BMC Genomics
Dorper sheep
Shedding
Hair cycle
RNA-seq
Hair follicle
Transcriptome analysis
title Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles
title_full Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles
title_fullStr Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles
title_full_unstemmed Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles
title_short Transcriptomic analysis of regulatory mechanisms in the telogen-anagen transition of ovine hair follicles
title_sort transcriptomic analysis of regulatory mechanisms in the telogen anagen transition of ovine hair follicles
topic Dorper sheep
Shedding
Hair cycle
RNA-seq
Hair follicle
Transcriptome analysis
url https://doi.org/10.1186/s12864-024-11059-7
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