Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide
Abstract The growing concerns over food safety have intensified calls for alternatives to toxic pesticides in agriculture. Despite these concerns, the global agricultural industry remains heavily reliant on chemical pesticides to maintain crop yields. However, the overuse of these chemicals has resu...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-01-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-024-00724-4 |
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| author | Jianyou Gao Jiewei Li Cuixia Liu Hongjuan Gong Beibei Qi Rongxiang Zhu Liming Xia Li Li Shibiao Liu Qiaosheng Jiang Kaiyu Ye Faming Wang |
| author_facet | Jianyou Gao Jiewei Li Cuixia Liu Hongjuan Gong Beibei Qi Rongxiang Zhu Liming Xia Li Li Shibiao Liu Qiaosheng Jiang Kaiyu Ye Faming Wang |
| author_sort | Jianyou Gao |
| collection | DOAJ |
| description | Abstract The growing concerns over food safety have intensified calls for alternatives to toxic pesticides in agriculture. Despite these concerns, the global agricultural industry remains heavily reliant on chemical pesticides to maintain crop yields. However, the overuse of these chemicals has resulted in significant biodiversity loss and environmental degradation, highlighting the urgent need for safer, non-toxic, and sustainable alternatives. Trichloroisocyanuric acid (TCCA), a cost-effective and relatively safe industrial oxidant commonly used for disinfection, has shown potential for plant disease management. However, its application in this context remains largely unexplored. In this study, we evaluate the efficacy of TCCA in controlling Pseudomonas syringae pv. actinidiae (Psa), the causative agent of bacterial canker in kiwifruit, and explore its underlying mechanisms of action. Our results demonstrate that TCCA effectively inhibits Psa growth in vitro, even at low concentrations, with minimum inhibitory concentrations (MICs) of 20 mg/L (TCCA added directly to the Psa suspension) and 100 mg/L (Psa cells mixed with liquid LB medium before adding TCC). In vivo, TCCA treatment at a concentration of 500 mg/L substantially reduced Psa colonization on both kiwifruit leaves and canes, outperforming conventional bactericides such as copper hydroxide, chlorothalonil, and ethylicin, as well as alternative treatments like pyraclostrobin-dysonline and Xinjunan acetate. Mechanistic investigations revealed that TCCA inhibited bacterial biofilm formation, impaired motility, disrupted cell integrity, and suppressed the expression of virulence-related genes, ultimately leading to bacterial cell death. Additionally, TCCA treatment of both healthy and infected canes induced the activity of key defense-related enzymes, including catalase (CAT), peroxidase (POD), glutathione reductase (GR), and phenylalanine ammonia-lyase (PAL), suggesting that TCCA may activate systemic plant defense responses. These findings position TCCA as a promising, environmentally friendly alternative to traditional toxic bactericides, offering a sustainable and effective solution for plant disease management with reduced ecological risks. Graphical Abstract |
| format | Article |
| id | doaj-art-bbf58cf2be174bc4bdc49e7759e67c41 |
| institution | Kabale University |
| issn | 2196-5641 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj-art-bbf58cf2be174bc4bdc49e7759e67c412025-01-12T12:11:07ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412025-01-0112111510.1186/s40538-024-00724-4Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericideJianyou Gao0Jiewei Li1Cuixia Liu2Hongjuan Gong3Beibei Qi4Rongxiang Zhu5Liming Xia6Li Li7Shibiao Liu8Qiaosheng Jiang9Kaiyu Ye10Faming Wang11Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design, Chinese Academy of SciencesCollege of Biology and Environmental Sciences, Jishou UniversityGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, , Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of SciencesAbstract The growing concerns over food safety have intensified calls for alternatives to toxic pesticides in agriculture. Despite these concerns, the global agricultural industry remains heavily reliant on chemical pesticides to maintain crop yields. However, the overuse of these chemicals has resulted in significant biodiversity loss and environmental degradation, highlighting the urgent need for safer, non-toxic, and sustainable alternatives. Trichloroisocyanuric acid (TCCA), a cost-effective and relatively safe industrial oxidant commonly used for disinfection, has shown potential for plant disease management. However, its application in this context remains largely unexplored. In this study, we evaluate the efficacy of TCCA in controlling Pseudomonas syringae pv. actinidiae (Psa), the causative agent of bacterial canker in kiwifruit, and explore its underlying mechanisms of action. Our results demonstrate that TCCA effectively inhibits Psa growth in vitro, even at low concentrations, with minimum inhibitory concentrations (MICs) of 20 mg/L (TCCA added directly to the Psa suspension) and 100 mg/L (Psa cells mixed with liquid LB medium before adding TCC). In vivo, TCCA treatment at a concentration of 500 mg/L substantially reduced Psa colonization on both kiwifruit leaves and canes, outperforming conventional bactericides such as copper hydroxide, chlorothalonil, and ethylicin, as well as alternative treatments like pyraclostrobin-dysonline and Xinjunan acetate. Mechanistic investigations revealed that TCCA inhibited bacterial biofilm formation, impaired motility, disrupted cell integrity, and suppressed the expression of virulence-related genes, ultimately leading to bacterial cell death. Additionally, TCCA treatment of both healthy and infected canes induced the activity of key defense-related enzymes, including catalase (CAT), peroxidase (POD), glutathione reductase (GR), and phenylalanine ammonia-lyase (PAL), suggesting that TCCA may activate systemic plant defense responses. These findings position TCCA as a promising, environmentally friendly alternative to traditional toxic bactericides, offering a sustainable and effective solution for plant disease management with reduced ecological risks. Graphical Abstracthttps://doi.org/10.1186/s40538-024-00724-4Trichloroisocyanuric acidBactericideSustainable agricultureVirulence genesDefense-related enzymesPseudomonas syringae pv. actinidiae (Psa) |
| spellingShingle | Jianyou Gao Jiewei Li Cuixia Liu Hongjuan Gong Beibei Qi Rongxiang Zhu Liming Xia Li Li Shibiao Liu Qiaosheng Jiang Kaiyu Ye Faming Wang Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide Chemical and Biological Technologies in Agriculture Trichloroisocyanuric acid Bactericide Sustainable agriculture Virulence genes Defense-related enzymes Pseudomonas syringae pv. actinidiae (Psa) |
| title | Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide |
| title_full | Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide |
| title_fullStr | Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide |
| title_full_unstemmed | Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide |
| title_short | Application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco-friendly bactericide |
| title_sort | application of trichloroisocyanuric acid in controlling kiwifruit bacterial canker disease demonstrates its promising potential as an eco friendly bactericide |
| topic | Trichloroisocyanuric acid Bactericide Sustainable agriculture Virulence genes Defense-related enzymes Pseudomonas syringae pv. actinidiae (Psa) |
| url | https://doi.org/10.1186/s40538-024-00724-4 |
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