Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin
ObjectiveStudies have shown that the exercise-induced cytokine Irisin functions were as a dimer, so tandem Irisin-Irisin mock dimers were designed to explore the similarities and differences between recombinant Irisin-Irisin expressed by <italic>Escherichia coli </italic>and commercial I...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Office of Rehabilitation Medicine
2023-02-01
|
| Series: | 康复学报 |
| Subjects: | |
| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2023.01005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841536895413125120 |
|---|---|
| author | LIU Fengying HUANG Yide LIN Jia CHEN Xuequn WANG Qingshui LIN Yao |
| author_facet | LIU Fengying HUANG Yide LIN Jia CHEN Xuequn WANG Qingshui LIN Yao |
| author_sort | LIU Fengying |
| collection | DOAJ |
| description | ObjectiveStudies have shown that the exercise-induced cytokine Irisin functions were as a dimer, so tandem Irisin-Irisin mock dimers were designed to explore the similarities and differences between recombinant Irisin-Irisin expressed by <italic>Escherichia coli </italic>and commercial Irisin in activity and function on tumor cells.Methods<italic>Escherichia coli</italic> strain BL21 (DE3) and pET-30a expression vectors were selected to obtain recombinant Irisin-Irisin by isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. High purity recombinant Irisin-Irisin was obtained by Ni Sepharose excel. C2C12 skeletal muscle cells were treated with recombinant Irisin-Irisin and monomeric Irisin respectively, and the expressions of mitochondrial related gene UCP-1 were observed to test the activity. The functions were verified in gastric cancer, colorectal cancer and other gastrointestinal tumors, and the activity and function of recombinant Irisin-Irisin and monomeric Irisin on tumor cells were compared.Results(1) Expression and purification of recombinant Irisin-Irisin: compared with the non-induced samples, the recombinant Irisin-Irisin was significantly expressed after IPTG induction; high purity recombinant Irisin-Irisin with a molecular weight of about 27 kDa was obtained by Ni Sepharose excel. (2) Detection of recombinant Irisin-Irisin activity: compared with the blank control group, both the recombinant Irisin-Irisin group and the Irisin group could up-regulate the expression of UCP-1 in C2C12 cells (<italic>P</italic><0.05); compared with the Irisin group, the recombinant Irisin-Irisin group had a more significant effect and stronger activity in inducing UCP-1 (<italic>P</italic><0.05). (3) Recombinant Irisin-Irisin on tumor function: compared with the blank control group, both the 100 ng/mL and 200 ng/mL Irisin-Irisin groups could inhibit the migration of gastric cancer cells and colorectal cancer cells(<italic>P</italic><0.05), but had no effect on their proliferation and clone formation; compared with the Irisin group, the recombinant Irisin-Irisin group had a more significant effect on cell migration inhibition (<italic>P</italic><0.05).ConclusionThe recombinant Irisin-Irisin expressed by <italic>Escherichia coli</italic> has activity, and the activity is higher than that of the monomeric Irisin; recombinant Irisin-Irisin has an effect on the migration of gastric cancer cells and colorectal cancer cells, and the effect was better than that of the monomeric Irisin. |
| format | Article |
| id | doaj-art-bb8bb050ddf24ad39352297919e3a4d2 |
| institution | Kabale University |
| issn | 2096-0328 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Editorial Office of Rehabilitation Medicine |
| record_format | Article |
| series | 康复学报 |
| spelling | doaj-art-bb8bb050ddf24ad39352297919e3a4d22025-01-14T10:08:00ZengEditorial Office of Rehabilitation Medicine康复学报2096-03282023-02-0133324135118222Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-IrisinLIU FengyingHUANG YideLIN JiaCHEN XuequnWANG QingshuiLIN YaoObjectiveStudies have shown that the exercise-induced cytokine Irisin functions were as a dimer, so tandem Irisin-Irisin mock dimers were designed to explore the similarities and differences between recombinant Irisin-Irisin expressed by <italic>Escherichia coli </italic>and commercial Irisin in activity and function on tumor cells.Methods<italic>Escherichia coli</italic> strain BL21 (DE3) and pET-30a expression vectors were selected to obtain recombinant Irisin-Irisin by isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. High purity recombinant Irisin-Irisin was obtained by Ni Sepharose excel. C2C12 skeletal muscle cells were treated with recombinant Irisin-Irisin and monomeric Irisin respectively, and the expressions of mitochondrial related gene UCP-1 were observed to test the activity. The functions were verified in gastric cancer, colorectal cancer and other gastrointestinal tumors, and the activity and function of recombinant Irisin-Irisin and monomeric Irisin on tumor cells were compared.Results(1) Expression and purification of recombinant Irisin-Irisin: compared with the non-induced samples, the recombinant Irisin-Irisin was significantly expressed after IPTG induction; high purity recombinant Irisin-Irisin with a molecular weight of about 27 kDa was obtained by Ni Sepharose excel. (2) Detection of recombinant Irisin-Irisin activity: compared with the blank control group, both the recombinant Irisin-Irisin group and the Irisin group could up-regulate the expression of UCP-1 in C2C12 cells (<italic>P</italic><0.05); compared with the Irisin group, the recombinant Irisin-Irisin group had a more significant effect and stronger activity in inducing UCP-1 (<italic>P</italic><0.05). (3) Recombinant Irisin-Irisin on tumor function: compared with the blank control group, both the 100 ng/mL and 200 ng/mL Irisin-Irisin groups could inhibit the migration of gastric cancer cells and colorectal cancer cells(<italic>P</italic><0.05), but had no effect on their proliferation and clone formation; compared with the Irisin group, the recombinant Irisin-Irisin group had a more significant effect on cell migration inhibition (<italic>P</italic><0.05).ConclusionThe recombinant Irisin-Irisin expressed by <italic>Escherichia coli</italic> has activity, and the activity is higher than that of the monomeric Irisin; recombinant Irisin-Irisin has an effect on the migration of gastric cancer cells and colorectal cancer cells, and the effect was better than that of the monomeric Irisin.http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2023.01005recombinant Irisin-Irisinexercise-induced cytokineprokaryotic expressionbiological function |
| spellingShingle | LIU Fengying HUANG Yide LIN Jia CHEN Xuequn WANG Qingshui LIN Yao Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin 康复学报 recombinant Irisin-Irisin exercise-induced cytokine prokaryotic expression biological function |
| title | Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin |
| title_full | Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin |
| title_fullStr | Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin |
| title_full_unstemmed | Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin |
| title_short | Expression and Function of Synthetic Dimeric Exercise-Induced Cytokine Irisin-Irisin |
| title_sort | expression and function of synthetic dimeric exercise induced cytokine irisin irisin |
| topic | recombinant Irisin-Irisin exercise-induced cytokine prokaryotic expression biological function |
| url | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2023.01005 |
| work_keys_str_mv | AT liufengying expressionandfunctionofsyntheticdimericexerciseinducedcytokineirisinirisin AT huangyide expressionandfunctionofsyntheticdimericexerciseinducedcytokineirisinirisin AT linjia expressionandfunctionofsyntheticdimericexerciseinducedcytokineirisinirisin AT chenxuequn expressionandfunctionofsyntheticdimericexerciseinducedcytokineirisinirisin AT wangqingshui expressionandfunctionofsyntheticdimericexerciseinducedcytokineirisinirisin AT linyao expressionandfunctionofsyntheticdimericexerciseinducedcytokineirisinirisin |