Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients

Abstract The COVID-19 pandemic has been the largest pandemic of the past century, and various genetic factors have played a significant role in this context. This study aimed to analyze the frequency and association between specific SNPs rs3806268 (NLRP3), rs4925543 (NLRP3), rs12150220 (NLRP1), rs45...

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Main Authors: PABLO C.S. FARIAS, LEANDRO P. CABRAL, PATRÍCIA A.F. NEVES, CAIO A.B. JANUÁRIO, BEATRIZ M. CORDEIRO, WILSON J. DA SILVA JÚNIOR, CAROLINA BASEGGIO, SÉRGIO S.L. PAIVA JÚNIOR, PAULO S.R. DE ARAÚJO, VIRGÍNIA MARIA B. DE LORENA, VALDIR Q. BALBINO, REGINALDO G. LIMA NETO
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Language:English
Published: Academia Brasileira de Ciências 2024-11-01
Series:Anais da Academia Brasileira de Ciências
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Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000700702&lng=en&tlng=en
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author PABLO C.S. FARIAS
LEANDRO P. CABRAL
PATRÍCIA A.F. NEVES
CAIO A.B. JANUÁRIO
BEATRIZ M. CORDEIRO
WILSON J. DA SILVA JÚNIOR
CAROLINA BASEGGIO
SÉRGIO S.L. PAIVA JÚNIOR
PAULO S.R. DE ARAÚJO
VIRGÍNIA MARIA B. DE LORENA
VALDIR Q. BALBINO
REGINALDO G. LIMA NETO
author_facet PABLO C.S. FARIAS
LEANDRO P. CABRAL
PATRÍCIA A.F. NEVES
CAIO A.B. JANUÁRIO
BEATRIZ M. CORDEIRO
WILSON J. DA SILVA JÚNIOR
CAROLINA BASEGGIO
SÉRGIO S.L. PAIVA JÚNIOR
PAULO S.R. DE ARAÚJO
VIRGÍNIA MARIA B. DE LORENA
VALDIR Q. BALBINO
REGINALDO G. LIMA NETO
author_sort PABLO C.S. FARIAS
collection DOAJ
description Abstract The COVID-19 pandemic has been the largest pandemic of the past century, and various genetic factors have played a significant role in this context. This study aimed to analyze the frequency and association between specific SNPs rs3806268 (NLRP3), rs4925543 (NLRP3), rs12150220 (NLRP1), rs455060 (NLRC4), rs699 (AGT), rs1137101 (LEPR), and rs1801133 (MTHFR) and severe/critical outcomes in Brazilian patients with COVID-19. A total of 100 patients were included in the study, comprising 66 cases and 34 controls. DNA was extracted, sequenced, and genotyped via next-generation sequencing (NGS). For non-parametric data, the Mann-Whitney and Kruskal-Wallis tests were used. Fisher’s test and multivariate logistic regression, considering AIC and BIC criteria, were employed for risk analysis. Odds Ratios (OR) were calculated, with significance set at p<0.05. Among the seven evaluated SNPs, only rs699-GG (AGT) (OR=8.07; p=0.04) was significantly associated with an increased risk of developing severe/critical COVID-19. Moreover, a borderline protective association was noted between rs1801133-GA (MTHFR) and the disease, although lacking statistical significance. In conclusion, the SNP rs699-GG (AGT) was associated with an increased risk of severe/critical COVID-19.
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spelling doaj-art-bb6a936e0b5c4803a29b3057653e21b92024-11-26T07:46:19ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902024-11-0196suppl 310.1590/0001-3765202420240274Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patientsPABLO C.S. FARIAShttps://orcid.org/0000-0001-8734-3468LEANDRO P. CABRALhttps://orcid.org/0000-0002-4094-1345PATRÍCIA A.F. NEVEShttps://orcid.org/0009-0005-8152-3827CAIO A.B. JANUÁRIOhttps://orcid.org/0000-0002-8916-9165BEATRIZ M. CORDEIROhttps://orcid.org/0009-0008-5089-319XWILSON J. DA SILVA JÚNIORhttps://orcid.org/0000-0002-0217-7665CAROLINA BASEGGIOhttps://orcid.org/0009-0002-1958-7043SÉRGIO S.L. PAIVA JÚNIORhttps://orcid.org/0000-0002-9938-6673PAULO S.R. DE ARAÚJOhttps://orcid.org/0000-0002-7839-0737VIRGÍNIA MARIA B. DE LORENAhttps://orcid.org/0000-0003-0663-236XVALDIR Q. BALBINOhttps://orcid.org/0000-0002-4319-3952REGINALDO G. LIMA NETOhttps://orcid.org/0000-0002-8846-877XAbstract The COVID-19 pandemic has been the largest pandemic of the past century, and various genetic factors have played a significant role in this context. This study aimed to analyze the frequency and association between specific SNPs rs3806268 (NLRP3), rs4925543 (NLRP3), rs12150220 (NLRP1), rs455060 (NLRC4), rs699 (AGT), rs1137101 (LEPR), and rs1801133 (MTHFR) and severe/critical outcomes in Brazilian patients with COVID-19. A total of 100 patients were included in the study, comprising 66 cases and 34 controls. DNA was extracted, sequenced, and genotyped via next-generation sequencing (NGS). For non-parametric data, the Mann-Whitney and Kruskal-Wallis tests were used. Fisher’s test and multivariate logistic regression, considering AIC and BIC criteria, were employed for risk analysis. Odds Ratios (OR) were calculated, with significance set at p<0.05. Among the seven evaluated SNPs, only rs699-GG (AGT) (OR=8.07; p=0.04) was significantly associated with an increased risk of developing severe/critical COVID-19. Moreover, a borderline protective association was noted between rs1801133-GA (MTHFR) and the disease, although lacking statistical significance. In conclusion, the SNP rs699-GG (AGT) was associated with an increased risk of severe/critical COVID-19.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000700702&lng=en&tlng=enAGT geneCOVID-19human geneticsSARS-CoV-2SNPs
spellingShingle PABLO C.S. FARIAS
LEANDRO P. CABRAL
PATRÍCIA A.F. NEVES
CAIO A.B. JANUÁRIO
BEATRIZ M. CORDEIRO
WILSON J. DA SILVA JÚNIOR
CAROLINA BASEGGIO
SÉRGIO S.L. PAIVA JÚNIOR
PAULO S.R. DE ARAÚJO
VIRGÍNIA MARIA B. DE LORENA
VALDIR Q. BALBINO
REGINALDO G. LIMA NETO
Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients
Anais da Academia Brasileira de Ciências
AGT gene
COVID-19
human genetics
SARS-CoV-2
SNPs
title Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients
title_full Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients
title_fullStr Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients
title_full_unstemmed Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients
title_short Genetic variant in the AGT gene (rs699-GG) is associated with severe COVID-19 in Brazilian patients
title_sort genetic variant in the agt gene rs699 gg is associated with severe covid 19 in brazilian patients
topic AGT gene
COVID-19
human genetics
SARS-CoV-2
SNPs
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000700702&lng=en&tlng=en
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