Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets
This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentratio...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Veterinary Quarterly |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2024.2447600 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841560654092173312 |
|---|---|
| author | Miao Zhou Zenghao Yan Deqin Wang Chuyuan Li Leli Wang Rui Li Jie Yin Yulong Yin |
| author_facet | Miao Zhou Zenghao Yan Deqin Wang Chuyuan Li Leli Wang Rui Li Jie Yin Yulong Yin |
| author_sort | Miao Zhou |
| collection | DOAJ |
| description | This study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores (p < 0.01). Notably, the serum levels of immunoglobulin A and immunoglobulin G increased linearly and quadratically (p < 0.01), while immunoglobulin M also showed a linear increase (p < 0.05) in IRP-fed piglets. The secretory immunoglobulin A levels in ileal contents were significantly higher compared to control piglets (p < 0.01). Key intestinal health parameters, including villus height, villus height-to-crypt depth ratio, and goblet cell numbers, showed linear and quadratic increases in both the jejunum and ileum (p < 0.05), while crypt depth decreased significantly (p < 0.01). Additionally, the expression of IL-10, ZO-1, occludin, and mucin2 was upregulated linearly and quadratically in IRP-fed piglets (p < 0.05). In cultured IPEC-J2 cells, ZO-1 and occludin expression levels significantly increased upon exposure to 400 μg/mL IRP (p < 0.01). Furthermore, the relative abundances of Escherichia coli, Ralstonia pickettii, and Desulfovibrio fairfieldensis decreased linearly with increasing dietary IRP concentration. In conclusion, IRP shows promise as an effective dietary supplement for mitigating diarrhea and enhancing intestinal health in early weaned piglets. |
| format | Article |
| id | doaj-art-bb3e741f544e4299988ca7b1fb331c90 |
| institution | Kabale University |
| issn | 0165-2176 1875-5941 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj-art-bb3e741f544e4299988ca7b1fb331c902025-01-03T21:48:07ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412025-12-0145111510.1080/01652176.2024.2447600Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in pigletsMiao Zhou0Zenghao Yan1Deqin Wang2Chuyuan Li3Leli Wang4Rui Li5Jie Yin6Yulong Yin7Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaHutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd, Guangzhou, ChinaHutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd, Guangzhou, ChinaHutchison Whampoa Guangzhou Baiyunshan Chinese Medicine Co., Ltd, Guangzhou, ChinaInstitute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaInstitute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaAnimal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, ChinaThis study aimed to investigate the effects of dietary isatidis root polysaccharide (IRP) on diarrhea, immunity, and intestinal health in weanling piglets. Forty healthy piglets were randomly assigned to five groups receiving varying dosages of IRP. The findings indicated that different concentrations of IRP significantly reduced diarrhea scores (p < 0.01). Notably, the serum levels of immunoglobulin A and immunoglobulin G increased linearly and quadratically (p < 0.01), while immunoglobulin M also showed a linear increase (p < 0.05) in IRP-fed piglets. The secretory immunoglobulin A levels in ileal contents were significantly higher compared to control piglets (p < 0.01). Key intestinal health parameters, including villus height, villus height-to-crypt depth ratio, and goblet cell numbers, showed linear and quadratic increases in both the jejunum and ileum (p < 0.05), while crypt depth decreased significantly (p < 0.01). Additionally, the expression of IL-10, ZO-1, occludin, and mucin2 was upregulated linearly and quadratically in IRP-fed piglets (p < 0.05). In cultured IPEC-J2 cells, ZO-1 and occludin expression levels significantly increased upon exposure to 400 μg/mL IRP (p < 0.01). Furthermore, the relative abundances of Escherichia coli, Ralstonia pickettii, and Desulfovibrio fairfieldensis decreased linearly with increasing dietary IRP concentration. In conclusion, IRP shows promise as an effective dietary supplement for mitigating diarrhea and enhancing intestinal health in early weaned piglets.https://www.tandfonline.com/doi/10.1080/01652176.2024.2447600Isatidis root polysaccharidesearly weaning stressdiarrheaintestinal healthgut microbiota |
| spellingShingle | Miao Zhou Zenghao Yan Deqin Wang Chuyuan Li Leli Wang Rui Li Jie Yin Yulong Yin Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets Veterinary Quarterly Isatidis root polysaccharides early weaning stress diarrhea intestinal health gut microbiota |
| title | Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets |
| title_full | Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets |
| title_fullStr | Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets |
| title_full_unstemmed | Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets |
| title_short | Isatidis root polysaccharides ameliorates post-weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets |
| title_sort | isatidis root polysaccharides ameliorates post weaning diarrhea by promoting intestinal health and modulating the gut microbiota in piglets |
| topic | Isatidis root polysaccharides early weaning stress diarrhea intestinal health gut microbiota |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2024.2447600 |
| work_keys_str_mv | AT miaozhou isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT zenghaoyan isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT deqinwang isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT chuyuanli isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT leliwang isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT ruili isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT jieyin isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets AT yulongyin isatidisrootpolysaccharidesamelioratespostweaningdiarrheabypromotingintestinalhealthandmodulatingthegutmicrobiotainpiglets |