Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome
INTRODUCTION: The present study was undertaken to elucidate the expression status and molecular mechanism underlying microRNA-3127-5p (miR-3127-5p) in polycystic ovary syndrome (PCOS). MATERIAL AND METHODS: A total of 50 PCOS and 50 non-PCOS patients were recruited as research subjects. Quantitative...
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Via Medica
2025-08-01
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| Series: | Endokrynologia Polska |
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| Online Access: | https://journals.viamedica.pl/endokrynologia_polska/article/view/104875 |
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| author | Hongjuan Zhang Ling Peng Lei Yao |
| author_facet | Hongjuan Zhang Ling Peng Lei Yao |
| author_sort | Hongjuan Zhang |
| collection | DOAJ |
| description | INTRODUCTION: The present study was undertaken to elucidate the expression status and molecular mechanism underlying microRNA-3127-5p (miR-3127-5p) in polycystic ovary syndrome (PCOS). MATERIAL AND METHODS: A total of 50 PCOS and 50 non-PCOS patients were recruited as research subjects. Quantitative real-time polymerase chain reaction was employed to assess the relative abundances of miR-3127-5p in serum, cumulus cells (CCs), and granulosa cells (GCs) from PCOS patients. Additionally, cellular activities and ferroptosis-related biomarkers were evaluated. Bioinformatics analysis was conducted to identify potential targets of miR-3127-5p. To validate the interaction between miR-3127-5p and sorbin and SH3 domain-containing protein 1 (SORBS1), a luciferase reporter assay was conducted. RESULTS: Enforced miR-3127-5p expression was detected in serum, CCs, and GCs from PCOS patients, demonstrating a robust diagnostic capacity for effectively distinguishing between PCOS and non-PCOS patients. Furthermore, the reduction of miR-3127-5p expression was found to enhance cell viability and inhibit cell apoptosis in human granulosa-like tumor cell line (KGN) and the luteinized granulosa cell line (SVOG) cells. Concurrently, its down-regulation led to attenuated levels of iron, malondialdehyde (MDA), and reactive oxygen species (ROS), while simultaneously increasing the expression of glutathione peroxidase 4 (GPX4). Notably, miR-3127-5p expression exhibited an inverse relationship with SORBS1. Rescue experiments revealed that the effects of downregulated miR-3127-5p expression on cellular behaviors and ferroptosis were reserved through the transfection of si-SORBS1. CONCLUSION: The downregulation of miR-3127-5p expression facilitates cell growth and attenuates ferroptosis by targeting SORBS1, thereby playing a pivotal role in the initiation and development of PCOS. |
| format | Article |
| id | doaj-art-bb1d34d0c6d84f52859cf6bf5d4924c6 |
| institution | Kabale University |
| issn | 0423-104X 2299-8306 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Via Medica |
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| series | Endokrynologia Polska |
| spelling | doaj-art-bb1d34d0c6d84f52859cf6bf5d4924c62025-08-21T06:40:59ZengVia MedicaEndokrynologia Polska0423-104X2299-83062025-08-0176410.5603/ep.104875Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndromeHongjuan Zhang0Ling Peng1Lei Yao2Department of Gynecology, The Fourth Hospital of Shijiazhuang, Shijiazhuang, ChinaDepartment of Gynecology, Chongqing Public Health Medical Center, Chongqing, ChinaDepartment of Gynecology, Geriatric Hospital Affiliated with Wuhan University of Science and Technology, Wuhan, ChinaINTRODUCTION: The present study was undertaken to elucidate the expression status and molecular mechanism underlying microRNA-3127-5p (miR-3127-5p) in polycystic ovary syndrome (PCOS). MATERIAL AND METHODS: A total of 50 PCOS and 50 non-PCOS patients were recruited as research subjects. Quantitative real-time polymerase chain reaction was employed to assess the relative abundances of miR-3127-5p in serum, cumulus cells (CCs), and granulosa cells (GCs) from PCOS patients. Additionally, cellular activities and ferroptosis-related biomarkers were evaluated. Bioinformatics analysis was conducted to identify potential targets of miR-3127-5p. To validate the interaction between miR-3127-5p and sorbin and SH3 domain-containing protein 1 (SORBS1), a luciferase reporter assay was conducted. RESULTS: Enforced miR-3127-5p expression was detected in serum, CCs, and GCs from PCOS patients, demonstrating a robust diagnostic capacity for effectively distinguishing between PCOS and non-PCOS patients. Furthermore, the reduction of miR-3127-5p expression was found to enhance cell viability and inhibit cell apoptosis in human granulosa-like tumor cell line (KGN) and the luteinized granulosa cell line (SVOG) cells. Concurrently, its down-regulation led to attenuated levels of iron, malondialdehyde (MDA), and reactive oxygen species (ROS), while simultaneously increasing the expression of glutathione peroxidase 4 (GPX4). Notably, miR-3127-5p expression exhibited an inverse relationship with SORBS1. Rescue experiments revealed that the effects of downregulated miR-3127-5p expression on cellular behaviors and ferroptosis were reserved through the transfection of si-SORBS1. CONCLUSION: The downregulation of miR-3127-5p expression facilitates cell growth and attenuates ferroptosis by targeting SORBS1, thereby playing a pivotal role in the initiation and development of PCOS.https://journals.viamedica.pl/endokrynologia_polska/article/view/104875MiR-3127-5ppolycystic ovary syndromecellular activitiesferroptosis |
| spellingShingle | Hongjuan Zhang Ling Peng Lei Yao Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome Endokrynologia Polska MiR-3127-5p polycystic ovary syndrome cellular activities ferroptosis |
| title | Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome |
| title_full | Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome |
| title_fullStr | Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome |
| title_full_unstemmed | Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome |
| title_short | Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome |
| title_sort | differential expression of microrna 3127 5p and its molecular mechanisms in polycystic ovary syndrome |
| topic | MiR-3127-5p polycystic ovary syndrome cellular activities ferroptosis |
| url | https://journals.viamedica.pl/endokrynologia_polska/article/view/104875 |
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