First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China

IntroductionPapillomaviruses have been previously identified in mammals, avians and fish. However, few numbers of reptiles’ PVs have been characterized.MethodsThis study investigated the oral cavity of the splendid japalure (Japalura splendida) in southwestern China using high-throughput sequencing....

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Main Authors: Zhige Tian, Tingjie Li, Yuping Fan, Jiayi Li, Sirong Luo, Wanxin Cao, Qing Pan, Xiaoliang Hu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1590538/full
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author Zhige Tian
Tingjie Li
Yuping Fan
Jiayi Li
Sirong Luo
Wanxin Cao
Qing Pan
Xiaoliang Hu
author_facet Zhige Tian
Tingjie Li
Yuping Fan
Jiayi Li
Sirong Luo
Wanxin Cao
Qing Pan
Xiaoliang Hu
author_sort Zhige Tian
collection DOAJ
description IntroductionPapillomaviruses have been previously identified in mammals, avians and fish. However, few numbers of reptiles’ PVs have been characterized.MethodsThis study investigated the oral cavity of the splendid japalure (Japalura splendida) in southwestern China using high-throughput sequencing. The presence of papillomavirus strain JsPV in oral samples was confirmed using PCR with consensus primers.Results and discussionIn this study, a papillomavirus strain, designated JsPV, in the oral cavity of the splendid japalure (Japalura splendida) in southwestern China. The complete JsPV genome was sequenced, comprising  222 bp. Phylogenetic analysis based on the L1 protein revealed that JsPV clustered closely with gecko-derived strains (HfrePV1 and HfrePV2) and other sauropsid-associated papillomaviruses, while remaining distinct from mammalian- and fish-associated lineages. These findings provide insights into the evolutionary origins of papillomaviruses in reptiles.
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publishDate 2025-07-01
publisher Frontiers Media S.A.
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series Frontiers in Microbiology
spelling doaj-art-bb1904778b8f4f8c83aae9545aaf4a1e2025-08-20T03:58:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15905381590538First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern ChinaZhige Tian0Tingjie Li1Yuping Fan2Jiayi Li3Sirong Luo4Wanxin Cao5Qing Pan6Xiaoliang Hu7Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaIntroductionPapillomaviruses have been previously identified in mammals, avians and fish. However, few numbers of reptiles’ PVs have been characterized.MethodsThis study investigated the oral cavity of the splendid japalure (Japalura splendida) in southwestern China using high-throughput sequencing. The presence of papillomavirus strain JsPV in oral samples was confirmed using PCR with consensus primers.Results and discussionIn this study, a papillomavirus strain, designated JsPV, in the oral cavity of the splendid japalure (Japalura splendida) in southwestern China. The complete JsPV genome was sequenced, comprising  222 bp. Phylogenetic analysis based on the L1 protein revealed that JsPV clustered closely with gecko-derived strains (HfrePV1 and HfrePV2) and other sauropsid-associated papillomaviruses, while remaining distinct from mammalian- and fish-associated lineages. These findings provide insights into the evolutionary origins of papillomaviruses in reptiles.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1590538/fullevolutionJapalura splendidalizardpapillomavirusreptile
spellingShingle Zhige Tian
Tingjie Li
Yuping Fan
Jiayi Li
Sirong Luo
Wanxin Cao
Qing Pan
Xiaoliang Hu
First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China
Frontiers in Microbiology
evolution
Japalura splendida
lizard
papillomavirus
reptile
title First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China
title_full First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China
title_fullStr First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China
title_full_unstemmed First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China
title_short First detection of a lizard-associated papillomavirus in the splendid japalure (Japalura splendida) from southwestern China
title_sort first detection of a lizard associated papillomavirus in the splendid japalure japalura splendida from southwestern china
topic evolution
Japalura splendida
lizard
papillomavirus
reptile
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1590538/full
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