The studies on virus isolation from feces in calves
In this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As...
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Selcuk University Press
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Series: | Eurasian Journal of Veterinary Sciences |
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Online Access: | http://eurasianjvetsci.org/pdf.php3?id=298 |
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author | Sibel Yavru Atilla Şimşek |
author_facet | Sibel Yavru Atilla Şimşek |
author_sort | Sibel Yavru |
collection | DOAJ |
description | In this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As a result of inoculation of 100 feces samples onto foetal calf kidney cell cultures, 2 viruses were isolated. The isolated viruses were identified as BAV-1 by cross microneutralization test with rabbit hyperimmun sera. Blood serum samples from these animals were tested using microneutralization test tor neutralizing antibody to BAV 1, 2 and 3. The presence of neutralizing antibody was detected in 17 out of 100 blood sera (17%) against BAV-1, in 21 (21%) against BAV-2 and in 23 (23%) against BAV-3. Serum neutralization 50 (SNso) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/ 14.1-1/531 for BAV-1,2 and 3 respectively. |
format | Article |
id | doaj-art-bb175a327847466f82a393ec50b2bfbd |
institution | Kabale University |
issn | 1309-6958 2146-1953 |
language | English |
publisher | Selcuk University Press |
record_format | Article |
series | Eurasian Journal of Veterinary Sciences |
spelling | doaj-art-bb175a327847466f82a393ec50b2bfbd2025-01-03T01:35:49ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19531735158298The studies on virus isolation from feces in calvesSibel YavruAtilla ŞimşekIn this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As a result of inoculation of 100 feces samples onto foetal calf kidney cell cultures, 2 viruses were isolated. The isolated viruses were identified as BAV-1 by cross microneutralization test with rabbit hyperimmun sera. Blood serum samples from these animals were tested using microneutralization test tor neutralizing antibody to BAV 1, 2 and 3. The presence of neutralizing antibody was detected in 17 out of 100 blood sera (17%) against BAV-1, in 21 (21%) against BAV-2 and in 23 (23%) against BAV-3. Serum neutralization 50 (SNso) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/ 14.1-1/531 for BAV-1,2 and 3 respectively.http://eurasianjvetsci.org/pdf.php3?id=298bovine adenovirus type-12 and 3fecescell cultureisolationneutralization test |
spellingShingle | Sibel Yavru Atilla Şimşek The studies on virus isolation from feces in calves Eurasian Journal of Veterinary Sciences bovine adenovirus type-1 2 and 3 feces cell culture isolation neutralization test |
title | The studies on virus isolation from feces in calves |
title_full | The studies on virus isolation from feces in calves |
title_fullStr | The studies on virus isolation from feces in calves |
title_full_unstemmed | The studies on virus isolation from feces in calves |
title_short | The studies on virus isolation from feces in calves |
title_sort | studies on virus isolation from feces in calves |
topic | bovine adenovirus type-1 2 and 3 feces cell culture isolation neutralization test |
url | http://eurasianjvetsci.org/pdf.php3?id=298 |
work_keys_str_mv | AT sibelyavru thestudiesonvirusisolationfromfecesincalves AT atillasimsek thestudiesonvirusisolationfromfecesincalves AT sibelyavru studiesonvirusisolationfromfecesincalves AT atillasimsek studiesonvirusisolationfromfecesincalves |