The studies on virus isolation from feces in calves

In this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As...

Full description

Saved in:
Bibliographic Details
Main Authors: Sibel Yavru, Atilla Şimşek
Format: Article
Language:English
Published: Selcuk University Press
Series:Eurasian Journal of Veterinary Sciences
Subjects:
Online Access:http://eurasianjvetsci.org/pdf.php3?id=298
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1841561512810905600
author Sibel Yavru
Atilla Şimşek
author_facet Sibel Yavru
Atilla Şimşek
author_sort Sibel Yavru
collection DOAJ
description In this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As a result of inoculation of 100 feces samples onto foetal calf kidney cell cultures, 2 viruses were isolated. The isolated viruses were identified as BAV-1 by cross microneutralization test with rabbit hyperimmun sera. Blood serum samples from these animals were tested using microneutralization test tor neutralizing antibody to BAV 1, 2 and 3. The presence of neutralizing antibody was detected in 17 out of 100 blood sera (17%) against BAV-1, in 21 (21%) against BAV-2 and in 23 (23%) against BAV-3. Serum neutralization 50 (SNso) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/ 14.1-1/531 for BAV-1,2 and 3 respectively.
format Article
id doaj-art-bb175a327847466f82a393ec50b2bfbd
institution Kabale University
issn 1309-6958
2146-1953
language English
publisher Selcuk University Press
record_format Article
series Eurasian Journal of Veterinary Sciences
spelling doaj-art-bb175a327847466f82a393ec50b2bfbd2025-01-03T01:35:49ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19531735158298The studies on virus isolation from feces in calvesSibel YavruAtilla ŞimşekIn this study, 100 feces samples from 0-1 old year calves with clinical sign of respiratory and digestive systems, fever and acut diarrhae symptom in eight private managements of Konya and its arround were used for virus isolation studies in sensitive cell cultures and checked for BAV 1,2 and 3. As a result of inoculation of 100 feces samples onto foetal calf kidney cell cultures, 2 viruses were isolated. The isolated viruses were identified as BAV-1 by cross microneutralization test with rabbit hyperimmun sera. Blood serum samples from these animals were tested using microneutralization test tor neutralizing antibody to BAV 1, 2 and 3. The presence of neutralizing antibody was detected in 17 out of 100 blood sera (17%) against BAV-1, in 21 (21%) against BAV-2 and in 23 (23%) against BAV-3. Serum neutralization 50 (SNso) values of seropositive animals were detected to be 1/14.1-1/531, 1/14.1-1/316 and 1/ 14.1-1/531 for BAV-1,2 and 3 respectively.http://eurasianjvetsci.org/pdf.php3?id=298bovine adenovirus type-12 and 3fecescell cultureisolationneutralization test
spellingShingle Sibel Yavru
Atilla Şimşek
The studies on virus isolation from feces in calves
Eurasian Journal of Veterinary Sciences
bovine adenovirus type-1
2 and 3
feces
cell culture
isolation
neutralization test
title The studies on virus isolation from feces in calves
title_full The studies on virus isolation from feces in calves
title_fullStr The studies on virus isolation from feces in calves
title_full_unstemmed The studies on virus isolation from feces in calves
title_short The studies on virus isolation from feces in calves
title_sort studies on virus isolation from feces in calves
topic bovine adenovirus type-1
2 and 3
feces
cell culture
isolation
neutralization test
url http://eurasianjvetsci.org/pdf.php3?id=298
work_keys_str_mv AT sibelyavru thestudiesonvirusisolationfromfecesincalves
AT atillasimsek thestudiesonvirusisolationfromfecesincalves
AT sibelyavru studiesonvirusisolationfromfecesincalves
AT atillasimsek studiesonvirusisolationfromfecesincalves