Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin
Purpose: In this work, the effects of sodium chloride (NaCl) on gene expression of planktonic Streptococcus mutans cells are investigated. Also assessed are the effects of NaCl on zeta potential of sound and demineralized dentin. Methods: The relative level of glucosyltransferase B (gtfB), gtfC and...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221242681830143X |
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| author | Ali Akbar Nagavi-alhoseiny Maryam Torshabi Morteza Rasoulianboroujeni Lobat Tayebi Fahimeh Sadat Tabatabaei |
| author_facet | Ali Akbar Nagavi-alhoseiny Maryam Torshabi Morteza Rasoulianboroujeni Lobat Tayebi Fahimeh Sadat Tabatabaei |
| author_sort | Ali Akbar Nagavi-alhoseiny |
| collection | DOAJ |
| description | Purpose: In this work, the effects of sodium chloride (NaCl) on gene expression of planktonic Streptococcus mutans cells are investigated. Also assessed are the effects of NaCl on zeta potential of sound and demineralized dentin. Methods: The relative level of glucosyltransferase B (gtfB), gtfC and gtfD transcription of S. mutans in the presence of NaCl was evaluated by quantitative polymerase chain reaction (qPCR). The osmolality of varying salt (NaCl) concentrations and their influence on the zeta potential of sound and demineralized dentin was investigated as well. Results: NaCl significantly reduced the expression of gtfB and C genes in planktonic S. mutans; whereas, gtf D gene expression significantly increased in the presence of NaCl (P < 0.05). NaCl at concentrations of 37.5 mg/ml reduced zeta potential of demineralized dentin, while no significant decrease of zeta potential was found when sound dentin was exposed to this concentration. Conclusion: NaCl reduces the expression of some gtfs in S. mutans and increases negative potential charge of demineralized dentin. |
| format | Article |
| id | doaj-art-bafbc0ea406248a0a5c1a96c928e4d25 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-bafbc0ea406248a0a5c1a96c928e4d252024-11-23T06:27:38ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682019-01-019114Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentinAli Akbar Nagavi-alhoseiny0Maryam Torshabi1Morteza Rasoulianboroujeni2Lobat Tayebi3Fahimeh Sadat Tabatabaei4Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, IranMarquette University, School of Dentistry, Milwaukee, WI, 53233, USAMarquette University, School of Dentistry, Milwaukee, WI, 53233, USADental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Marquette University, School of Dentistry, Milwaukee, WI, 53233, USA; Corresponding author. Marquette University, School of Dentistry, Milwaukee, WI, 53233, USA.Purpose: In this work, the effects of sodium chloride (NaCl) on gene expression of planktonic Streptococcus mutans cells are investigated. Also assessed are the effects of NaCl on zeta potential of sound and demineralized dentin. Methods: The relative level of glucosyltransferase B (gtfB), gtfC and gtfD transcription of S. mutans in the presence of NaCl was evaluated by quantitative polymerase chain reaction (qPCR). The osmolality of varying salt (NaCl) concentrations and their influence on the zeta potential of sound and demineralized dentin was investigated as well. Results: NaCl significantly reduced the expression of gtfB and C genes in planktonic S. mutans; whereas, gtf D gene expression significantly increased in the presence of NaCl (P < 0.05). NaCl at concentrations of 37.5 mg/ml reduced zeta potential of demineralized dentin, while no significant decrease of zeta potential was found when sound dentin was exposed to this concentration. Conclusion: NaCl reduces the expression of some gtfs in S. mutans and increases negative potential charge of demineralized dentin.http://www.sciencedirect.com/science/article/pii/S221242681830143XGlycosylteransferaseOsmolalitySodium chlorideStreptococcus mutansZeta potential |
| spellingShingle | Ali Akbar Nagavi-alhoseiny Maryam Torshabi Morteza Rasoulianboroujeni Lobat Tayebi Fahimeh Sadat Tabatabaei Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin Journal of Oral Biology and Craniofacial Research Glycosylteransferase Osmolality Sodium chloride Streptococcus mutans Zeta potential |
| title | Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin |
| title_full | Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin |
| title_fullStr | Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin |
| title_full_unstemmed | Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin |
| title_short | Effect of sodium chloride on gene expression of Streptococcus mutans and zeta potential of demineralized dentin |
| title_sort | effect of sodium chloride on gene expression of streptococcus mutans and zeta potential of demineralized dentin |
| topic | Glycosylteransferase Osmolality Sodium chloride Streptococcus mutans Zeta potential |
| url | http://www.sciencedirect.com/science/article/pii/S221242681830143X |
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