Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep
Limosilactobacillus reuteri is a species of lactobacillus present in the gastrointestinal tract of animals and may contribute to the balance of intestinal flora. This study aimed to investigate the effects and correlations of L. reuteri on both intestinal flora and muscle fiber characteristics of Su...
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| Language: | English |
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Tsinghua University Press
2024-12-01
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| Series: | Food Science of Animal Products |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSAP.2024.9240096 |
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| author | Ting Liu Chenlei Wang Mirco Corazzin Taiwu Zhang Maoqin Zhai Yue Zhang Qiaoge Zhang Xin Zhao Ye Jin Lina Sun |
| author_facet | Ting Liu Chenlei Wang Mirco Corazzin Taiwu Zhang Maoqin Zhai Yue Zhang Qiaoge Zhang Xin Zhao Ye Jin Lina Sun |
| author_sort | Ting Liu |
| collection | DOAJ |
| description | Limosilactobacillus reuteri is a species of lactobacillus present in the gastrointestinal tract of animals and may contribute to the balance of intestinal flora. This study aimed to investigate the effects and correlations of L. reuteri on both intestinal flora and muscle fiber characteristics of Sunit sheep, with the objective of enhancing meat quality. Twelve 8-month-old Sunit sheep were randomly divided into two groups: control (fed without L. reuteri) and L. reuteri group. The results showed that L. reuteri had a significant positive impact on meat quality, particularly improving the tenderness of sheep meat compared with the control group, with values of 69.8 and 76.8 N, respectively (P < 0.05). L. reuteri group showed significantly more type I muscle fibers (23% vs. 13%) and higher succinate dehydrogenase activity than the control group, while lactate dehydrogenase activity was significantly lower (P < 0.05). The L. reuteri group showed significantly higher expression of genes encoding colonic epithelial nutrient transport (GPR43) and immune barrier function (Zonulin) than the control group. At the genus level, Alistipes and Methanobacterium were significantly different between L. reuteri and control groups (P < 0.05), with L. reuteri group showing an increase of 2.9 times and a decrease of 8.0 times compared to the control group, respectively. At the species level, Succiniclasticum ruminis and Bacteroides sp. 43_108 were significantly (P < 0.05) lower and higher, respectively in L. reuteri group than those in the control group. Butyric acid exhibited the highest concentration in L. reuteri group, reaching 7.52 mmol/L. In conclusion, supplementation with L. reuteri may affect muscle fiber characteristics, enhance intestinal health, and facilitate carbohydrate digestion of Sunit sheep. This study offers novel insights into the use of feed additives for improving meat quality. |
| format | Article |
| id | doaj-art-bae69c9d709b4b5cb48fa8c4f8e153f8 |
| institution | Kabale University |
| issn | 2958-4124 2958-3780 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tsinghua University Press |
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| series | Food Science of Animal Products |
| spelling | doaj-art-bae69c9d709b4b5cb48fa8c4f8e153f82025-01-10T06:49:39ZengTsinghua University PressFood Science of Animal Products2958-41242958-37802024-12-0124924009610.26599/FSAP.2024.9240096Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheepTing Liu0Chenlei Wang1Mirco Corazzin2Taiwu Zhang3Maoqin Zhai4Yue Zhang5Qiaoge Zhang6Xin Zhao7Ye Jin8Lina Sun9College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine 33100, ItalyCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaCollege of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, ChinaLimosilactobacillus reuteri is a species of lactobacillus present in the gastrointestinal tract of animals and may contribute to the balance of intestinal flora. This study aimed to investigate the effects and correlations of L. reuteri on both intestinal flora and muscle fiber characteristics of Sunit sheep, with the objective of enhancing meat quality. Twelve 8-month-old Sunit sheep were randomly divided into two groups: control (fed without L. reuteri) and L. reuteri group. The results showed that L. reuteri had a significant positive impact on meat quality, particularly improving the tenderness of sheep meat compared with the control group, with values of 69.8 and 76.8 N, respectively (P < 0.05). L. reuteri group showed significantly more type I muscle fibers (23% vs. 13%) and higher succinate dehydrogenase activity than the control group, while lactate dehydrogenase activity was significantly lower (P < 0.05). The L. reuteri group showed significantly higher expression of genes encoding colonic epithelial nutrient transport (GPR43) and immune barrier function (Zonulin) than the control group. At the genus level, Alistipes and Methanobacterium were significantly different between L. reuteri and control groups (P < 0.05), with L. reuteri group showing an increase of 2.9 times and a decrease of 8.0 times compared to the control group, respectively. At the species level, Succiniclasticum ruminis and Bacteroides sp. 43_108 were significantly (P < 0.05) lower and higher, respectively in L. reuteri group than those in the control group. Butyric acid exhibited the highest concentration in L. reuteri group, reaching 7.52 mmol/L. In conclusion, supplementation with L. reuteri may affect muscle fiber characteristics, enhance intestinal health, and facilitate carbohydrate digestion of Sunit sheep. This study offers novel insights into the use of feed additives for improving meat quality.https://www.sciopen.com/article/10.26599/FSAP.2024.9240096limosilactobacillus reuterigut microbiotamuscle fiber typesmeat qualitysunit sheep |
| spellingShingle | Ting Liu Chenlei Wang Mirco Corazzin Taiwu Zhang Maoqin Zhai Yue Zhang Qiaoge Zhang Xin Zhao Ye Jin Lina Sun Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep Food Science of Animal Products limosilactobacillus reuteri gut microbiota muscle fiber types meat quality sunit sheep |
| title | Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep |
| title_full | Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep |
| title_fullStr | Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep |
| title_full_unstemmed | Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep |
| title_short | Effects of Limosilactobacillus reuteri on gastrointestinal microbiota, muscle fiber formation and meat quality of Sunit sheep |
| title_sort | effects of limosilactobacillus reuteri on gastrointestinal microbiota muscle fiber formation and meat quality of sunit sheep |
| topic | limosilactobacillus reuteri gut microbiota muscle fiber types meat quality sunit sheep |
| url | https://www.sciopen.com/article/10.26599/FSAP.2024.9240096 |
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