Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China
Introduction: Non-tuberculous mycobacterium (NTM) can colonize the human body, leading to opportunistic infection. This study was conducted to analyze the NTM species composition in a primary hospital and investigate the potential features of the patients with different NTM species. Methodology:...
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The Journal of Infection in Developing Countries
2019-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11772 |
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| author | Xinling Pan Yangxiao Zhou Zhaoxia Li Jun Zhang Li Hong Yunzhen Shi Guo-Gang Li |
| author_facet | Xinling Pan Yangxiao Zhou Zhaoxia Li Jun Zhang Li Hong Yunzhen Shi Guo-Gang Li |
| author_sort | Xinling Pan |
| collection | DOAJ |
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Introduction: Non-tuberculous mycobacterium (NTM) can colonize the human body, leading to opportunistic infection. This study was conducted to analyze the NTM species composition in a primary hospital and investigate the potential features of the patients with different NTM species.
Methodology: Mycobacterial strains were collected from the patients admitted at the hospital from January 2016 to May 2019. MPB64 assay was used to screen NTM strains and confirmed by Rv0577 amplification. The species were identified by hsp65 sequencing. The clinical records of patients with NTM were retrospectively reviewed.
Results: Among the 122 identified NTM isolates, the most common strains were Mycobacterium avium complex (MAC, n = 102, 83.6%), Mycobacterium abscessus (n = 9, 7.4%) and Mycobacterium lentiflavum (n = 5, 4.1%). The predominant species among MAC were Mycobacterium chimaera (n = 57, 46.7%), followed by Mycobacterium intracellulare (n = 25, 20.5%) and Mycobacterium colombiense (n = 17, 13.9%). A significantly lower percentage of positive acid-fast assay was observed in Mycobacterium colombiense positive patients than in those with Mycobacterium intracellulare and Mycobacterium chimaera. Mycobacterium intracellulare was more frequently isolated in patients from the infectious department than in other MAC members.
Conclusions: A predominant prevalence of Mycobacterium chimaera in Dongyang of Zhejiang Province was different from other regions in China, indicating that its prevalence has been likely underestimated. The heterogeneity in clinical features, caused by different MAC members, required an accurate species identification of the NTM isolated in the primary hospitals.
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| format | Article |
| id | doaj-art-bad6ecc31da9408d88a1d3c4f758ccf7 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-bad6ecc31da9408d88a1d3c4f758ccf72025-08-20T03:52:41ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-12-01131210.3855/jidc.11772Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern ChinaXinling Pan0Yangxiao Zhou1Zhaoxia Li2Jun Zhang3Li Hong4Yunzhen Shi5Guo-Gang Li6Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, ChinaDepartment of Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, ChinaDepartment of Infectious Diseases, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, ChinaClinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, ChinaDepartment of Infectious Diseases, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, ChinaDepartment of Infectious Diseases, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, ChinaDepartment of Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Dongyang, Zhejiang, China Introduction: Non-tuberculous mycobacterium (NTM) can colonize the human body, leading to opportunistic infection. This study was conducted to analyze the NTM species composition in a primary hospital and investigate the potential features of the patients with different NTM species. Methodology: Mycobacterial strains were collected from the patients admitted at the hospital from January 2016 to May 2019. MPB64 assay was used to screen NTM strains and confirmed by Rv0577 amplification. The species were identified by hsp65 sequencing. The clinical records of patients with NTM were retrospectively reviewed. Results: Among the 122 identified NTM isolates, the most common strains were Mycobacterium avium complex (MAC, n = 102, 83.6%), Mycobacterium abscessus (n = 9, 7.4%) and Mycobacterium lentiflavum (n = 5, 4.1%). The predominant species among MAC were Mycobacterium chimaera (n = 57, 46.7%), followed by Mycobacterium intracellulare (n = 25, 20.5%) and Mycobacterium colombiense (n = 17, 13.9%). A significantly lower percentage of positive acid-fast assay was observed in Mycobacterium colombiense positive patients than in those with Mycobacterium intracellulare and Mycobacterium chimaera. Mycobacterium intracellulare was more frequently isolated in patients from the infectious department than in other MAC members. Conclusions: A predominant prevalence of Mycobacterium chimaera in Dongyang of Zhejiang Province was different from other regions in China, indicating that its prevalence has been likely underestimated. The heterogeneity in clinical features, caused by different MAC members, required an accurate species identification of the NTM isolated in the primary hospitals. https://jidc.org/index.php/journal/article/view/11772non-tuberculous mycobacteriummycobacterium avium complexMycobacterium chimaeraMycobacterium colombienseMycobacterium intracellulare |
| spellingShingle | Xinling Pan Yangxiao Zhou Zhaoxia Li Jun Zhang Li Hong Yunzhen Shi Guo-Gang Li Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China Journal of Infection in Developing Countries non-tuberculous mycobacterium mycobacterium avium complex Mycobacterium chimaera Mycobacterium colombiense Mycobacterium intracellulare |
| title | Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China |
| title_full | Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China |
| title_fullStr | Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China |
| title_full_unstemmed | Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China |
| title_short | Investigation of non-tuberculous mycobacteria in a primary hospital from southeastern China |
| title_sort | investigation of non tuberculous mycobacteria in a primary hospital from southeastern china |
| topic | non-tuberculous mycobacterium mycobacterium avium complex Mycobacterium chimaera Mycobacterium colombiense Mycobacterium intracellulare |
| url | https://jidc.org/index.php/journal/article/view/11772 |
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