Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice
The purpose of the research is to study effects of Trichinella spiralis and Echinococcus multilocularis extracts on mitosis in a bone marrow cell population, and on hematological and biochemical blood parameters of mice after single and multiple intraperitoneal injections.Materials and methods. The...
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Federal Scientific Centre VIEV
2023-01-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/967 |
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| author | T. S. Novik E. I. Koveshnikova S. I. Chukina L. A. Napisanova O. N. Andreyanov I. A. Arkhipov K. G. Kurochkina |
| author_facet | T. S. Novik E. I. Koveshnikova S. I. Chukina L. A. Napisanova O. N. Andreyanov I. A. Arkhipov K. G. Kurochkina |
| author_sort | T. S. Novik |
| collection | DOAJ |
| description | The purpose of the research is to study effects of Trichinella spiralis and Echinococcus multilocularis extracts on mitosis in a bone marrow cell population, and on hematological and biochemical blood parameters of mice after single and multiple intraperitoneal injections.Materials and methods. The experiment was conducted on outbred male mice. The T. spiralis and E. multilocularis extracts were administered intraperitoneally once or multiple times daily for 10 days at a dose of 80 μg/mouse. Bone marrow cell isolation, microscopic preparations, mitotic index and individual stage determination were made as described in the literature (Ford C. E., Hamerton J. L., 1956). The mouse main peripheral blood parameters were determined with a MicroCC-20 Plus hematological analyzer, and the leucogram was determined by a conventional method. Biochemical blood parameters of the mice were determined with a Clima MC-15 analyzer.Results and discussion. The T. spiralis and E. multilocularis extracts after a single intraperitoneal injection at a dose of 80 μg/ mouse had a pronounced negative effect on the mouse bone marrow cell mitosis with the cell division terminated in the metaphase and a decreased proportion of other mitosis stages. Characteristics were detected of the effects made by the T. spiralis and E. multilocularis extracts on the mouse bone marrow cell mitosis after multiple administration for 10 days. In the reinjection mode of the test extracts to the mice, hematological and biochemical parameters did not change. |
| format | Article |
| id | doaj-art-bacb889d5d5947e89b0ad5e667be548f |
| institution | Kabale University |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| publishDate | 2023-01-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj-art-bacb889d5d5947e89b0ad5e667be548f2025-08-20T03:59:17ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432023-01-0116441142010.31016/1998-8435-2022-16-4-411-420714Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of miceT. S. Novik0E. I. Koveshnikova1S. I. Chukina2L. A. Napisanova3O. N. Andreyanov4I. A. Arkhipov5K. G. Kurochkina6All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"The purpose of the research is to study effects of Trichinella spiralis and Echinococcus multilocularis extracts on mitosis in a bone marrow cell population, and on hematological and biochemical blood parameters of mice after single and multiple intraperitoneal injections.Materials and methods. The experiment was conducted on outbred male mice. The T. spiralis and E. multilocularis extracts were administered intraperitoneally once or multiple times daily for 10 days at a dose of 80 μg/mouse. Bone marrow cell isolation, microscopic preparations, mitotic index and individual stage determination were made as described in the literature (Ford C. E., Hamerton J. L., 1956). The mouse main peripheral blood parameters were determined with a MicroCC-20 Plus hematological analyzer, and the leucogram was determined by a conventional method. Biochemical blood parameters of the mice were determined with a Clima MC-15 analyzer.Results and discussion. The T. spiralis and E. multilocularis extracts after a single intraperitoneal injection at a dose of 80 μg/ mouse had a pronounced negative effect on the mouse bone marrow cell mitosis with the cell division terminated in the metaphase and a decreased proportion of other mitosis stages. Characteristics were detected of the effects made by the T. spiralis and E. multilocularis extracts on the mouse bone marrow cell mitosis after multiple administration for 10 days. In the reinjection mode of the test extracts to the mice, hematological and biochemical parameters did not change.https://vniigis.elpub.ru/jour/article/view/967<i>trichinella spiralis</i><i>echinococcus multilocularis</i>single and multiple injectionmitosishematological parametersbiochemical parametersmice |
| spellingShingle | T. S. Novik E. I. Koveshnikova S. I. Chukina L. A. Napisanova O. N. Andreyanov I. A. Arkhipov K. G. Kurochkina Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice Российский паразитологический журнал <i>trichinella spiralis</i> <i>echinococcus multilocularis</i> single and multiple injection mitosis hematological parameters biochemical parameters mice |
| title | Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice |
| title_full | Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice |
| title_fullStr | Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice |
| title_full_unstemmed | Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice |
| title_short | Effects of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice |
| title_sort | effects of i trichinella spiralis i and i echinococcus multilocularis i extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice |
| topic | <i>trichinella spiralis</i> <i>echinococcus multilocularis</i> single and multiple injection mitosis hematological parameters biochemical parameters mice |
| url | https://vniigis.elpub.ru/jour/article/view/967 |
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