In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration
Objectives: Recent investigations indicate that canine periodontal ligament-derived stem cells (cPDLSCs) may reveal a reliable strategy for repair of periodontal tissues via cell-based tissue engineering approaches. Due to limited research, this study aimed to demonstrate the phenotypic characteriza...
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Elsevier
2023-05-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426823000374 |
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| author | Hamideh Salari Sedigh Anna Saffarpour Shahram Jamshidi Mahdi Ashouri Seyed Mahdi Nassiri Mohammad Mehdi Dehghan Esmail Ranjbar Reyhaneh Shafieian |
| author_facet | Hamideh Salari Sedigh Anna Saffarpour Shahram Jamshidi Mahdi Ashouri Seyed Mahdi Nassiri Mohammad Mehdi Dehghan Esmail Ranjbar Reyhaneh Shafieian |
| author_sort | Hamideh Salari Sedigh |
| collection | DOAJ |
| description | Objectives: Recent investigations indicate that canine periodontal ligament-derived stem cells (cPDLSCs) may reveal a reliable strategy for repair of periodontal tissues via cell-based tissue engineering approaches. Due to limited research, this study aimed to demonstrate the phenotypic characterization of cPDLSc in comparison with canine bone marrow-derived mesenchymal stem cells (cBMSCs) in vitro. Methods: Mesenchymal stem cells (MSCs) were obtained from PDL and BM of five male adult Mongrel dogs. In vitro isolation and expansion as well as biologic characterization including colony unit formation (CFU), osteogenic and adipogenic differentiation, flow cytometric analysis of CD34 and CD44, and RT-PCR of alkaline phosphatase (ALP), osteocalcin (OCN), periostin (POSTN) and S100A4 were performed. Furthermore, electron microscopy analysis was done to complement the comparative research. Results: CFU assay revealed that colonies of cPDLSCs presented 70% confluency with a more finite lifespan than BM-MSCs, showing a significant increase in cPDLSCs. Both types of MSCs showed osteogenic and adipogenic phenotypic characterized with clusters of mineralized depositions and lipid vacuoles, respectively. Both types of MSCs expressed CD44 with limited expression of CD34. RT-PCR of cPDLSCs revealed that expression of ALP, POSTN, OCN and S100A4 genes were significantly higher than those of BMSCs. In addition, comparison of SEM and revealed that cPDLSCs expressed more extracellular collagen fibers. Conclusions: The current study indicated that cPDLSCs show potency as a novel cellular therapy for periodontal regeneration a large animal model. |
| format | Article |
| id | doaj-art-baa2a4d5a90c4bb899cee5f6b301d999 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-baa2a4d5a90c4bb899cee5f6b301d9992024-11-23T06:30:06ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682023-05-01133403411In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regenerationHamideh Salari Sedigh0Anna Saffarpour1Shahram Jamshidi2Mahdi Ashouri3Seyed Mahdi Nassiri4Mohammad Mehdi Dehghan5Esmail Ranjbar6Reyhaneh Shafieian7Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Periodontology, Tehran University of Medical Sciences, International Campus, Tehran, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran; Corresponding author. Faculty of Veterinary Medicine, Department of Clinical Sciences, 16th Azar St., Enghelab Sq., University of Tehran, PO 1417466191, Tehran, Iran.Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University of Medical Sciences, Tehran, IranDepartment of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Surgery & Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Stem Cell and Regenerative Medicine Center, Mashhad University of Medical Sciences, Mashhad, Iran; Corresponding author. School of Medicine, Department of Anatomy and Cell Biology, VakilAbad Blv, Azadi Sq., Mashhad University of Medical Sciences, PO 91779-48564, Mashhad, Iran.Objectives: Recent investigations indicate that canine periodontal ligament-derived stem cells (cPDLSCs) may reveal a reliable strategy for repair of periodontal tissues via cell-based tissue engineering approaches. Due to limited research, this study aimed to demonstrate the phenotypic characterization of cPDLSc in comparison with canine bone marrow-derived mesenchymal stem cells (cBMSCs) in vitro. Methods: Mesenchymal stem cells (MSCs) were obtained from PDL and BM of five male adult Mongrel dogs. In vitro isolation and expansion as well as biologic characterization including colony unit formation (CFU), osteogenic and adipogenic differentiation, flow cytometric analysis of CD34 and CD44, and RT-PCR of alkaline phosphatase (ALP), osteocalcin (OCN), periostin (POSTN) and S100A4 were performed. Furthermore, electron microscopy analysis was done to complement the comparative research. Results: CFU assay revealed that colonies of cPDLSCs presented 70% confluency with a more finite lifespan than BM-MSCs, showing a significant increase in cPDLSCs. Both types of MSCs showed osteogenic and adipogenic phenotypic characterized with clusters of mineralized depositions and lipid vacuoles, respectively. Both types of MSCs expressed CD44 with limited expression of CD34. RT-PCR of cPDLSCs revealed that expression of ALP, POSTN, OCN and S100A4 genes were significantly higher than those of BMSCs. In addition, comparison of SEM and revealed that cPDLSCs expressed more extracellular collagen fibers. Conclusions: The current study indicated that cPDLSCs show potency as a novel cellular therapy for periodontal regeneration a large animal model.http://www.sciencedirect.com/science/article/pii/S2212426823000374CaninePeriodontal ligamentBone marrowMesenchymal stem cellsRegenerative medicine |
| spellingShingle | Hamideh Salari Sedigh Anna Saffarpour Shahram Jamshidi Mahdi Ashouri Seyed Mahdi Nassiri Mohammad Mehdi Dehghan Esmail Ranjbar Reyhaneh Shafieian In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration Journal of Oral Biology and Craniofacial Research Canine Periodontal ligament Bone marrow Mesenchymal stem cells Regenerative medicine |
| title | In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration |
| title_full | In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration |
| title_fullStr | In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration |
| title_full_unstemmed | In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration |
| title_short | In vitro investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration |
| title_sort | in vitro investigation of canine periodontal ligament derived mesenchymal stem cells a possibility of promising tool for periodontal regeneration |
| topic | Canine Periodontal ligament Bone marrow Mesenchymal stem cells Regenerative medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2212426823000374 |
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