Real-time fluorescence growth curves for viable bacteria quantification in foods
Here, for the first time, we used a membrane permeable fluorescent nucleic acid stain (SYBR Green) to trace the in-vivo DNA replication during bacterial binary fission. Such stain did not influence the growth of bacteria. Nor did the bacteria degrade the stain, enabling the fluorescent microplate re...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524007740 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846125274006028288 |
|---|---|
| author | Yajing Chen Yanlin Chen Siying Tang Biao Tang Shengbin He |
| author_facet | Yajing Chen Yanlin Chen Siying Tang Biao Tang Shengbin He |
| author_sort | Yajing Chen |
| collection | DOAJ |
| description | Here, for the first time, we used a membrane permeable fluorescent nucleic acid stain (SYBR Green) to trace the in-vivo DNA replication during bacterial binary fission. Such stain did not influence the growth of bacteria. Nor did the bacteria degrade the stain, enabling the fluorescent microplate reader to monitor sensitively the growth of the bacteria. Hence, a real-time fluorescence growth curve (RTFGC) method was put forward for the sensitive quantification of viable bacteria in foods. Using E. coli O157:H7 as a bacteria model, the RTFGC method could quantify bacteria within the range of 10 to 1 × 106 cfu/mL, with a linear correlation coefficient R2 of 0.997. It was found that melting curve was unique for a particular bacterial strain, which could be used for contamination identifications. Good practicability of the RTFGC in quantifying E. coli O157:H7 from tap water, juices, and milks was demonstrated. |
| format | Article |
| id | doaj-art-ba761a7227c04d5fb857b8d8c6c79c3b |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-ba761a7227c04d5fb857b8d8c6c79c3b2024-12-13T11:01:40ZengElsevierFood Chemistry: X2590-15752024-12-0124101886Real-time fluorescence growth curves for viable bacteria quantification in foodsYajing Chen0Yanlin Chen1Siying Tang2Biao Tang3Shengbin He4Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, PR ChinaKey Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, PR ChinaKey Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, PR ChinaKey Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, PR ChinaCorresponding author.; Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education, Guangxi Colleges and Universities Key Laboratory of Biological Molecular Medicine Research, School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi 530021, PR ChinaHere, for the first time, we used a membrane permeable fluorescent nucleic acid stain (SYBR Green) to trace the in-vivo DNA replication during bacterial binary fission. Such stain did not influence the growth of bacteria. Nor did the bacteria degrade the stain, enabling the fluorescent microplate reader to monitor sensitively the growth of the bacteria. Hence, a real-time fluorescence growth curve (RTFGC) method was put forward for the sensitive quantification of viable bacteria in foods. Using E. coli O157:H7 as a bacteria model, the RTFGC method could quantify bacteria within the range of 10 to 1 × 106 cfu/mL, with a linear correlation coefficient R2 of 0.997. It was found that melting curve was unique for a particular bacterial strain, which could be used for contamination identifications. Good practicability of the RTFGC in quantifying E. coli O157:H7 from tap water, juices, and milks was demonstrated.http://www.sciencedirect.com/science/article/pii/S2590157524007740Bacterial quantificationFood safety controlSYBR greenReal-timeFluorescence growth curve |
| spellingShingle | Yajing Chen Yanlin Chen Siying Tang Biao Tang Shengbin He Real-time fluorescence growth curves for viable bacteria quantification in foods Food Chemistry: X Bacterial quantification Food safety control SYBR green Real-time Fluorescence growth curve |
| title | Real-time fluorescence growth curves for viable bacteria quantification in foods |
| title_full | Real-time fluorescence growth curves for viable bacteria quantification in foods |
| title_fullStr | Real-time fluorescence growth curves for viable bacteria quantification in foods |
| title_full_unstemmed | Real-time fluorescence growth curves for viable bacteria quantification in foods |
| title_short | Real-time fluorescence growth curves for viable bacteria quantification in foods |
| title_sort | real time fluorescence growth curves for viable bacteria quantification in foods |
| topic | Bacterial quantification Food safety control SYBR green Real-time Fluorescence growth curve |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524007740 |
| work_keys_str_mv | AT yajingchen realtimefluorescencegrowthcurvesforviablebacteriaquantificationinfoods AT yanlinchen realtimefluorescencegrowthcurvesforviablebacteriaquantificationinfoods AT siyingtang realtimefluorescencegrowthcurvesforviablebacteriaquantificationinfoods AT biaotang realtimefluorescencegrowthcurvesforviablebacteriaquantificationinfoods AT shengbinhe realtimefluorescencegrowthcurvesforviablebacteriaquantificationinfoods |