An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i>
Fusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> (<i>Fop</i>) poses significant threats to pea cultivation worldwide. Controlling this disease is mainly achieved through the integration of various disease management procedures, among which biol...
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2024-11-01
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| author | Marco Masi Dorsaf Nedjar Moustafa Bani Ivana Staiano Maria Michela Salvatore Karima Khenaka Stefany Castaldi Jesus Garcia Zorrilla Anna Andolfi Rachele Isticato Alessio Cimmino |
| author_facet | Marco Masi Dorsaf Nedjar Moustafa Bani Ivana Staiano Maria Michela Salvatore Karima Khenaka Stefany Castaldi Jesus Garcia Zorrilla Anna Andolfi Rachele Isticato Alessio Cimmino |
| author_sort | Marco Masi |
| collection | DOAJ |
| description | Fusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> (<i>Fop</i>) poses significant threats to pea cultivation worldwide. Controlling this disease is mainly achieved through the integration of various disease management procedures, among which biological control has proven to be a safe and effective approach. This study aims to extract and identify antifungal secondary metabolites from <i>Streptomyces alboflavus</i> KRO3 strain and assess their effectiveness in inhibiting the in vitro growth of <i>Fop</i>. This bacterial strain exerts in vitro antagonistic activity against <i>Fop</i>, achieving highly significant inhibition over one week. The ethyl acetate extract, obtained from its ISP2 agar medium culture, also exhibited strong antifungal activity, maintaining an inhibition rate of approximately 90% at concentrations up to 250 µg/plug compared to the control. Thus, the organic extract has been fractionated using chromatographic techniques and its bioguided purification allowed us to isolate the main bioactive compound. This latter was identified as metacycloprodigiosin using nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and specific optical rotation data. Metacycloprodigiosin demonstrates dose-dependent inhibitory activity against the phytopathogen with an effective concentration of 125 µg/plug. The other secondary metabolites present in the ethyl acetate extract were also identified by gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). This study highlighted the potential of <i>S. alboflavus</i> KRO3 strain and its antimicrobial compounds for the management of the pea pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i>. |
| format | Article |
| id | doaj-art-ba3185abd2b84e2c9c1c1b242e836b4d |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-ba3185abd2b84e2c9c1c1b242e836b4d2024-11-26T18:08:52ZengMDPI AGJournal of Fungi2309-608X2024-11-01101178310.3390/jof10110783An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i>Marco Masi0Dorsaf Nedjar1Moustafa Bani2Ivana Staiano3Maria Michela Salvatore4Karima Khenaka5Stefany Castaldi6Jesus Garcia Zorrilla7Anna Andolfi8Rachele Isticato9Alessio Cimmino10Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyLaboratory of Biotechnology, Higher National School of Biotechnology Taoufik Khaznadar, Nouveau Pôle Universitaire Ali Mendjeli, BP. E66, Constantine 25100, AlgeriaLaboratory of Biotechnology, Higher National School of Biotechnology Taoufik Khaznadar, Nouveau Pôle Universitaire Ali Mendjeli, BP. E66, Constantine 25100, AlgeriaDepartment of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyLaboratory of Biotechnology, Higher National School of Biotechnology Taoufik Khaznadar, Nouveau Pôle Universitaire Ali Mendjeli, BP. E66, Constantine 25100, AlgeriaDepartment of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyDepartment of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cinthia 4, 80126 Naples, ItalyFusarium wilt caused by <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> (<i>Fop</i>) poses significant threats to pea cultivation worldwide. Controlling this disease is mainly achieved through the integration of various disease management procedures, among which biological control has proven to be a safe and effective approach. This study aims to extract and identify antifungal secondary metabolites from <i>Streptomyces alboflavus</i> KRO3 strain and assess their effectiveness in inhibiting the in vitro growth of <i>Fop</i>. This bacterial strain exerts in vitro antagonistic activity against <i>Fop</i>, achieving highly significant inhibition over one week. The ethyl acetate extract, obtained from its ISP2 agar medium culture, also exhibited strong antifungal activity, maintaining an inhibition rate of approximately 90% at concentrations up to 250 µg/plug compared to the control. Thus, the organic extract has been fractionated using chromatographic techniques and its bioguided purification allowed us to isolate the main bioactive compound. This latter was identified as metacycloprodigiosin using nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and specific optical rotation data. Metacycloprodigiosin demonstrates dose-dependent inhibitory activity against the phytopathogen with an effective concentration of 125 µg/plug. The other secondary metabolites present in the ethyl acetate extract were also identified by gas chromatography–mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). This study highlighted the potential of <i>S. alboflavus</i> KRO3 strain and its antimicrobial compounds for the management of the pea pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i>.https://www.mdpi.com/2309-608X/10/11/783fusarium wilt<i>Fusarium oxysporum</i><i>Streptomyces</i> sp.antimicrobial compoundsprodiginine natural productsmetacycloprodigiosin |
| spellingShingle | Marco Masi Dorsaf Nedjar Moustafa Bani Ivana Staiano Maria Michela Salvatore Karima Khenaka Stefany Castaldi Jesus Garcia Zorrilla Anna Andolfi Rachele Isticato Alessio Cimmino An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> Journal of Fungi fusarium wilt <i>Fusarium oxysporum</i> <i>Streptomyces</i> sp. antimicrobial compounds prodiginine natural products metacycloprodigiosin |
| title | An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> |
| title_full | An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> |
| title_fullStr | An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> |
| title_full_unstemmed | An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> |
| title_short | An Algerian Soil-Living <i>Streptomyces alboflavus</i> Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen <i>Fusarium oxysporum</i> f. sp. <i>pisi</i> |
| title_sort | algerian soil living i streptomyces alboflavus i strain as source of antifungal compounds for the management of the pea pathogen i fusarium oxysporum i f sp i pisi i |
| topic | fusarium wilt <i>Fusarium oxysporum</i> <i>Streptomyces</i> sp. antimicrobial compounds prodiginine natural products metacycloprodigiosin |
| url | https://www.mdpi.com/2309-608X/10/11/783 |
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