Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory
In scenarios when the morphological identification of samples is not feasible, the utilization of molecular analyses becomes an imperative. However, that can often be challenging in conditions where standard molecular laboratories cannot be established. In this study, we present a simplified and fie...
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MDPI AG
2024-10-01
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| Series: | Forensic Sciences |
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| Online Access: | https://www.mdpi.com/2673-6756/4/4/38 |
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| author | Karolina Mahlerová Lenka Vaňková Marcin Tomsia Daniel Vaněk |
| author_facet | Karolina Mahlerová Lenka Vaňková Marcin Tomsia Daniel Vaněk |
| author_sort | Karolina Mahlerová |
| collection | DOAJ |
| description | In scenarios when the morphological identification of samples is not feasible, the utilization of molecular analyses becomes an imperative. However, that can often be challenging in conditions where standard molecular laboratories cannot be established. In this study, we present a simplified and field-friendly protocol of the previously published Triplex PCR Assay for the identification of mammalian and plant sample origin using a fully portable molecular laboratory from Bento Lab (Bento Bioworks Ltd., London, UK), effectively eliminating the need for a traditional laboratory setup. The protocol in combination with correctly selected kits enables DNA extraction, result evaluation based on electrophoresis, and direct use of the PCR products for downstream analyses such as Sanger sequencing and third-generation sequencing; therefore, enabling the use of molecular analysis directly in situ or for educational purposes in a classroom. |
| format | Article |
| id | doaj-art-b9fb09c9d2244c8b809cd317d1adbd76 |
| institution | Kabale University |
| issn | 2673-6756 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forensic Sciences |
| spelling | doaj-art-b9fb09c9d2244c8b809cd317d1adbd762024-12-27T14:26:52ZengMDPI AGForensic Sciences2673-67562024-10-014456657210.3390/forensicsci4040038Technical Note: Rapid Species Barcoding Using Bento Lab Mobile LaboratoryKarolina Mahlerová0Lenka Vaňková1Marcin Tomsia2Daniel Vaněk3Institute for Environmental Studies, Charles University Prague, Benátská 2, 128 01 Prague, Czech RepublicInstitute for Environmental Studies, Charles University Prague, Benátská 2, 128 01 Prague, Czech RepublicDepartment of Forensic Medicine and Forensic Toxicology, Faculty of Medical Sciences (FOMS) in Katowice, Medical University of Silesia, 18 Medyków Street, 40-752 Katowice, PolandInstitute for Environmental Studies, Charles University Prague, Benátská 2, 128 01 Prague, Czech RepublicIn scenarios when the morphological identification of samples is not feasible, the utilization of molecular analyses becomes an imperative. However, that can often be challenging in conditions where standard molecular laboratories cannot be established. In this study, we present a simplified and field-friendly protocol of the previously published Triplex PCR Assay for the identification of mammalian and plant sample origin using a fully portable molecular laboratory from Bento Lab (Bento Bioworks Ltd., London, UK), effectively eliminating the need for a traditional laboratory setup. The protocol in combination with correctly selected kits enables DNA extraction, result evaluation based on electrophoresis, and direct use of the PCR products for downstream analyses such as Sanger sequencing and third-generation sequencing; therefore, enabling the use of molecular analysis directly in situ or for educational purposes in a classroom.https://www.mdpi.com/2673-6756/4/4/38Bento Labmobile laboratorybarcodingCOIRuBisCOin situ |
| spellingShingle | Karolina Mahlerová Lenka Vaňková Marcin Tomsia Daniel Vaněk Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory Forensic Sciences Bento Lab mobile laboratory barcoding COI RuBisCO in situ |
| title | Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory |
| title_full | Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory |
| title_fullStr | Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory |
| title_full_unstemmed | Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory |
| title_short | Technical Note: Rapid Species Barcoding Using Bento Lab Mobile Laboratory |
| title_sort | technical note rapid species barcoding using bento lab mobile laboratory |
| topic | Bento Lab mobile laboratory barcoding COI RuBisCO in situ |
| url | https://www.mdpi.com/2673-6756/4/4/38 |
| work_keys_str_mv | AT karolinamahlerova technicalnoterapidspeciesbarcodingusingbentolabmobilelaboratory AT lenkavankova technicalnoterapidspeciesbarcodingusingbentolabmobilelaboratory AT marcintomsia technicalnoterapidspeciesbarcodingusingbentolabmobilelaboratory AT danielvanek technicalnoterapidspeciesbarcodingusingbentolabmobilelaboratory |