Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>

Abstract  Aeromonas hydrophila is a motile Aeromonas bacteria that cause Septicemia (MAS) or the disease kwon as red spots (Plumb, 1994). These bacteria attack the various types of freshwater fish such as African catfish, carp and prawns. Treatment of MAS is still a lot of use antibiotics, whereas a...

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Main Author: Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya Rahardja
Format: Article
Language:English
Published: Faculty of Fisheries and Marine Universitas Airlangga 2012-04-01
Series:Jurnal Ilmiah Perikanan dan Kelautan
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Online Access:https://e-journal.unair.ac.id/JIPK/article/view/11584
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author Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya Rahardja
author_facet Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya Rahardja
author_sort Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya Rahardja
collection DOAJ
description Abstract  Aeromonas hydrophila is a motile Aeromonas bacteria that cause Septicemia (MAS) or the disease kwon as red spots (Plumb, 1994). These bacteria attack the various types of freshwater fish such as African catfish, carp and prawns. Treatment of MAS is still a lot of use antibiotics, whereas antibiotic a lot side effects. This study tries for find an alternative use  antibacterial substances using natural chemicals derived from medicinal plants of leaves extracs red betel (P. crocatum) which is antibacterial is a volatile oil, flavonoids, alkaloids and tannins.  The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of leaves  extracts red betel (P. crocatum) against of bacteria A. hydrophila. The research was conducted on July-August 2011, at the faculty of veterinary medicine, Airlangga University and Organic Chemistry Laboratory Faculty Of science and Technology Airlangga University. The method used in this study is an experimental method, using completely randomized design (RAL) with 11 treatments and three replications. Variables observed in this study is the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of leaves extracts red betel (P. crocatum). The observations were analyzed using analysis of variance (ANAVA). The results showed that extracts leaves red betel (P. crocatum) has efficacy against the bacteria A. hydrophila in vitro. Based on the results of MIC testing, leaves exracts of red betel (P. crocatum) inhibits growth of bacteria A. hydrophila at a concentration of 12,5% and based on test results of MBC, leaves extracs of red betel (P. crocatum) effectively kills the bacteria A. hydrophila at a concentration of 25%. It can be concluded that the concentration of antibacterial substances contained in red betel able to inhibit and kill bacteria A. hydrophila. This study recommends further to determine the effects of leaves extracts  red betel (P. crocatum) A. hydrophila against bacteria in vitro, so that can know the effectiveness of leaves effects of leaves extracts  red betel (P. crocatum) in suppressing populations of bacteria A. hydrophila
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institution Kabale University
issn 2085-5842
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language English
publishDate 2012-04-01
publisher Faculty of Fisheries and Marine Universitas Airlangga
record_format Article
series Jurnal Ilmiah Perikanan dan Kelautan
spelling doaj-art-b9f2762c1d5e45eaa1e4095c240d78262025-01-02T01:13:21ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592012-04-0141596410.20473/jipk.v4i1.115845851Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya RahardjaAbstract  Aeromonas hydrophila is a motile Aeromonas bacteria that cause Septicemia (MAS) or the disease kwon as red spots (Plumb, 1994). These bacteria attack the various types of freshwater fish such as African catfish, carp and prawns. Treatment of MAS is still a lot of use antibiotics, whereas antibiotic a lot side effects. This study tries for find an alternative use  antibacterial substances using natural chemicals derived from medicinal plants of leaves extracs red betel (P. crocatum) which is antibacterial is a volatile oil, flavonoids, alkaloids and tannins.  The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of leaves  extracts red betel (P. crocatum) against of bacteria A. hydrophila. The research was conducted on July-August 2011, at the faculty of veterinary medicine, Airlangga University and Organic Chemistry Laboratory Faculty Of science and Technology Airlangga University. The method used in this study is an experimental method, using completely randomized design (RAL) with 11 treatments and three replications. Variables observed in this study is the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of leaves extracts red betel (P. crocatum). The observations were analyzed using analysis of variance (ANAVA). The results showed that extracts leaves red betel (P. crocatum) has efficacy against the bacteria A. hydrophila in vitro. Based on the results of MIC testing, leaves exracts of red betel (P. crocatum) inhibits growth of bacteria A. hydrophila at a concentration of 12,5% and based on test results of MBC, leaves extracs of red betel (P. crocatum) effectively kills the bacteria A. hydrophila at a concentration of 25%. It can be concluded that the concentration of antibacterial substances contained in red betel able to inhibit and kill bacteria A. hydrophila. This study recommends further to determine the effects of leaves extracts  red betel (P. crocatum) A. hydrophila against bacteria in vitro, so that can know the effectiveness of leaves effects of leaves extracts  red betel (P. crocatum) in suppressing populations of bacteria A. hydrophilahttps://e-journal.unair.ac.id/JIPK/article/view/11584extracts red betel (piper crocatum), bacteria aeromonas hydrophila
spellingShingle Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya Rahardja
Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>
Jurnal Ilmiah Perikanan dan Kelautan
extracts red betel (piper crocatum), bacteria aeromonas hydrophila
title Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>
title_full Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>
title_fullStr Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>
title_full_unstemmed Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>
title_short Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro <br><i>[ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ]<i>
title_sort uji efektifitas ekstrak daun sirih merah piper rocatum terhadap bakteri aeromonas hydrophila secara in vitro br i effectines of leaves extracts red betel piper crocatum againts of bacteria aeromonas hydrophila in vitro i
topic extracts red betel (piper crocatum), bacteria aeromonas hydrophila
url https://e-journal.unair.ac.id/JIPK/article/view/11584
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