VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation
Myocardial fibrosis (MF) is an important cause of heart failure and cardiac arrest. Vasorin knockout (VASN−/−) leads to pathological cardiac hypertrophy (PCH); however, it is not yet clear whether this PCH transitions to MF in mice. VASN-knockout mice showed typical pathological, imaging, and molecu...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1500617/full |
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| author | Junming Sun Siwei Yin Qiurui Li Jun Zhang Xiaoping Guo Na Yu Bing Hu Yiqiang Ouyang Qiaojuan Huang Min He Min He Min He |
| author_facet | Junming Sun Siwei Yin Qiurui Li Jun Zhang Xiaoping Guo Na Yu Bing Hu Yiqiang Ouyang Qiaojuan Huang Min He Min He Min He |
| author_sort | Junming Sun |
| collection | DOAJ |
| description | Myocardial fibrosis (MF) is an important cause of heart failure and cardiac arrest. Vasorin knockout (VASN−/−) leads to pathological cardiac hypertrophy (PCH); however, it is not yet clear whether this PCH transitions to MF in mice. VASN-knockout mice showed typical pathological, imaging, and molecular features of MF upon hematoxylin and eosin staining, Masson staining, Sirius red staining, quantitative polymerase chain reaction (qPCR), immunohistochemistry-paraffin (IHC-P), and immunofluorescence analyses. RNA was extracted from mouse heart tissue, identified, and sequenced in vitro. Differential analysis of the genes showed that the extracellular matrix (ECM) genes (COL6A1, COL9A1, and FRAS1) had strong correlations while their expression levels were significantly reduced by qPCR, IHC-P, and Western blotting. The expression levels of the ECM genes were significantly reduced but those of the inflammatory factors (IL1β and IL6) were significantly upregulated in the heart tissues of VASN-knockout mice. These preliminary results reveal that VASN knockout induces MF by regulating the non-collagen fibers and inflammation. |
| format | Article |
| id | doaj-art-b9987c0dcabb45549eb7a47e37e4228c |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-b9987c0dcabb45549eb7a47e37e4228c2025-01-17T06:51:12ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15006171500617VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammationJunming Sun0Siwei Yin1Qiurui Li2Jun Zhang3Xiaoping Guo4Na Yu5Bing Hu6Yiqiang Ouyang7Qiaojuan Huang8Min He9Min He10Min He11Laboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaDepartment of Cardiology, The Second Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, ChinaLaboratory Animal Center, Guangxi Medical University, Nanning, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, Nanning, ChinaMinistry of Education, Key Laboratory of High-Incidence-Tumor Prevention and Treatment, Guangxi Medical University, Nanning, ChinaMyocardial fibrosis (MF) is an important cause of heart failure and cardiac arrest. Vasorin knockout (VASN−/−) leads to pathological cardiac hypertrophy (PCH); however, it is not yet clear whether this PCH transitions to MF in mice. VASN-knockout mice showed typical pathological, imaging, and molecular features of MF upon hematoxylin and eosin staining, Masson staining, Sirius red staining, quantitative polymerase chain reaction (qPCR), immunohistochemistry-paraffin (IHC-P), and immunofluorescence analyses. RNA was extracted from mouse heart tissue, identified, and sequenced in vitro. Differential analysis of the genes showed that the extracellular matrix (ECM) genes (COL6A1, COL9A1, and FRAS1) had strong correlations while their expression levels were significantly reduced by qPCR, IHC-P, and Western blotting. The expression levels of the ECM genes were significantly reduced but those of the inflammatory factors (IL1β and IL6) were significantly upregulated in the heart tissues of VASN-knockout mice. These preliminary results reveal that VASN knockout induces MF by regulating the non-collagen fibers and inflammation.https://www.frontiersin.org/articles/10.3389/fphar.2024.1500617/fullmyocardial fibrosisvasorinnon-collagen fibersinflammationmice |
| spellingShingle | Junming Sun Siwei Yin Qiurui Li Jun Zhang Xiaoping Guo Na Yu Bing Hu Yiqiang Ouyang Qiaojuan Huang Min He Min He Min He VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation Frontiers in Pharmacology myocardial fibrosis vasorin non-collagen fibers inflammation mice |
| title | VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation |
| title_full | VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation |
| title_fullStr | VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation |
| title_full_unstemmed | VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation |
| title_short | VASN knockout induces myocardial fibrosis in mice by downregulating non-collagen fibers and promoting inflammation |
| title_sort | vasn knockout induces myocardial fibrosis in mice by downregulating non collagen fibers and promoting inflammation |
| topic | myocardial fibrosis vasorin non-collagen fibers inflammation mice |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1500617/full |
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