Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection
BACKGROUND: Vaginal candidiasis is one of the most important fungal diseases in humans. Butternut squash (B. squash) has antifungal effects. This study investigated the effect of alcoholic extracts of B. squash on Candida albicans (C. albicans). METHODS: In this laboratory experiment, B. squash u...
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Vesnu Publications
2024-10-01
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| Series: | Chronic Diseases Journal |
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| Online Access: | http://cdjournal.muk.ac.ir/index.php/cdj/article/view/952 |
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| author | Azam Ghorbannia-Delaver Issa Gholampour-Azizi Atefeh Bozorgi Faezeh Hassanzadeh |
| author_facet | Azam Ghorbannia-Delaver Issa Gholampour-Azizi Atefeh Bozorgi Faezeh Hassanzadeh |
| author_sort | Azam Ghorbannia-Delaver |
| collection | DOAJ |
| description | BACKGROUND: Vaginal candidiasis is one of the most important fungal diseases in humans. Butternut squash (B. squash) has antifungal effects. This study investigated the effect of alcoholic extracts of B. squash on Candida albicans (C. albicans).
METHODS: In this laboratory experiment, B. squash using convenient sampling was collected from Babol City, Iran, in 2019. Determining the sensitivity of C. albicans to alcoholic extracts was tested using the disk, well, and minimum fungicidal concentration (MFC) methods and through determining the minimum inhibitory concentrations (MICs). Gas chromatography (GC) was used to determine the effective substances of the chemical compounds of B. squash extract. SPSS software, two-way analysis of variance, multiple comparisons, and independent samples t-test were used for data analysis.
RESULTS: Around the disk with 70 g/ml of ethanol extract (10 mm) and methanol (11 mm), and the wells with 110 mg/ml of methanol extract (17 mm), the average growth halo was greater. The size of the non-growth halo and the increasing trend of halo diameter between different alcoholic groups increased with increasing extract concentrations, but this increase was not significant (P < 0.05). In all 3 extracts, MIC was observed in 5-9 tubes and MFC in 3-9. The highest chemical composition was related to 5-hydroxymethylfurfural with 83.62%.
CONCLUSION: Based on the results of this study, B. squash alcoholic extracts effectively inhibit C. albicans and can be used as a promising tool to control or treat fungal diseases. |
| format | Article |
| id | doaj-art-b98794172a2b46fbb13be5f77e565630 |
| institution | Kabale University |
| issn | 2588-7297 2345-2226 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Vesnu Publications |
| record_format | Article |
| series | Chronic Diseases Journal |
| spelling | doaj-art-b98794172a2b46fbb13be5f77e5656302024-12-09T07:33:04ZengVesnu PublicationsChronic Diseases Journal2588-72972345-22262024-10-01124241248https://doi.org/10.22122/cdj.v12i4.952Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infectionAzam Ghorbannia-Delaver0https://orcid.org/0000-0002-7954-7038Issa Gholampour-Azizi1https://orcid.org/0000-0002-3950-8093Atefeh Bozorgi2https://orcid.org/0000-0002-8076-7281Faezeh Hassanzadeh3https://orcid.org/0000-0002-9089-2203Department of Biology, Payam Noor University, Tehran, IranDepartment of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, IranDepartment of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, IranDepartment of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, IranBACKGROUND: Vaginal candidiasis is one of the most important fungal diseases in humans. Butternut squash (B. squash) has antifungal effects. This study investigated the effect of alcoholic extracts of B. squash on Candida albicans (C. albicans). METHODS: In this laboratory experiment, B. squash using convenient sampling was collected from Babol City, Iran, in 2019. Determining the sensitivity of C. albicans to alcoholic extracts was tested using the disk, well, and minimum fungicidal concentration (MFC) methods and through determining the minimum inhibitory concentrations (MICs). Gas chromatography (GC) was used to determine the effective substances of the chemical compounds of B. squash extract. SPSS software, two-way analysis of variance, multiple comparisons, and independent samples t-test were used for data analysis. RESULTS: Around the disk with 70 g/ml of ethanol extract (10 mm) and methanol (11 mm), and the wells with 110 mg/ml of methanol extract (17 mm), the average growth halo was greater. The size of the non-growth halo and the increasing trend of halo diameter between different alcoholic groups increased with increasing extract concentrations, but this increase was not significant (P < 0.05). In all 3 extracts, MIC was observed in 5-9 tubes and MFC in 3-9. The highest chemical composition was related to 5-hydroxymethylfurfural with 83.62%. CONCLUSION: Based on the results of this study, B. squash alcoholic extracts effectively inhibit C. albicans and can be used as a promising tool to control or treat fungal diseases.http://cdjournal.muk.ac.ir/index.php/cdj/article/view/952antifungalbutternut squashcandida albicansvaginal infection |
| spellingShingle | Azam Ghorbannia-Delaver Issa Gholampour-Azizi Atefeh Bozorgi Faezeh Hassanzadeh Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection Chronic Diseases Journal antifungal butternut squash candida albicans vaginal infection |
| title | Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection |
| title_full | Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection |
| title_fullStr | Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection |
| title_full_unstemmed | Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection |
| title_short | Investigating the antifungal effect of alcoholic extract of Butternut squash on Candida albicans isolated from vaginal infection |
| title_sort | investigating the antifungal effect of alcoholic extract of butternut squash on candida albicans isolated from vaginal infection |
| topic | antifungal butternut squash candida albicans vaginal infection |
| url | http://cdjournal.muk.ac.ir/index.php/cdj/article/view/952 |
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