Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy
The aim of this study was to evaluate a robust magnetic resonance (MR) vessel size imaging (VSI) method for the noninvasive assessment of mean vessel size in solid tumors in a clinical dose range of ultrasmall superparamagnetic particles of iron oxide (USPIO). Therefore, USPIO-enhanced MR-VSI was pe...
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SAGE Publishing
2013-10-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2013.00059 |
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| author | Thorsten Persigehl Janine Ring Tymoteusz Budny Anke Hahnenkamp Sandra Stoeppeler Lawrence H. Schwartz Hans-Ullrich Spiegel Walter Heindel Stefanie Remmele Christoph Bremer |
| author_facet | Thorsten Persigehl Janine Ring Tymoteusz Budny Anke Hahnenkamp Sandra Stoeppeler Lawrence H. Schwartz Hans-Ullrich Spiegel Walter Heindel Stefanie Remmele Christoph Bremer |
| author_sort | Thorsten Persigehl |
| collection | DOAJ |
| description | The aim of this study was to evaluate a robust magnetic resonance (MR) vessel size imaging (VSI) method for the noninvasive assessment of mean vessel size in solid tumors in a clinical dose range of ultrasmall superparamagnetic particles of iron oxide (USPIO). Therefore, USPIO-enhanced MR-VSI was performed on DU-4475, MDA-MB-435, and EOMA tumor–bearing mice xenografts with known differences in angiogenic activity and vessel morphology. MR results were compared to vessel sizes determined by immunohistochemistry (anti-CD31) and by intravital microscopy (IVM). MR-VSI revealed significantly different mean vessel sizes between the xenograft models at both USPIO doses (DU-4475: 20.6 ± 4.9 mm; MDA-MB-435: 37.4 ± 8.8 μm; and EOMA: 60.3 ± 9.6 μm at 80 μmol/kg; p < .05). Immunohistochemistry revealed lower values for all tumor entities, whereas the size distribution was in line with MR-measurements. IVM corroborated the MR results for DU-4475 and MDA-MB435, but showed similar vessel sizes for MDA-MB-435 and EOMA. Our MR-VSI method allowed a noninvasive estimation of the mean vessel size in mice xenograft solid tumors with variable vascularity using a clinically relevant USPIO dose range. |
| format | Article |
| id | doaj-art-b97af071a0a94d08aa40f95d89d42305 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2013-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-b97af071a0a94d08aa40f95d89d423052025-01-03T00:11:02ZengSAGE PublishingMolecular Imaging1536-01212013-10-011210.2310/7290.2013.0005910.2310_7290.2013.00059Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital MicroscopyThorsten PersigehlJanine RingTymoteusz BudnyAnke HahnenkampSandra StoeppelerLawrence H. SchwartzHans-Ullrich SpiegelWalter HeindelStefanie RemmeleChristoph BremerThe aim of this study was to evaluate a robust magnetic resonance (MR) vessel size imaging (VSI) method for the noninvasive assessment of mean vessel size in solid tumors in a clinical dose range of ultrasmall superparamagnetic particles of iron oxide (USPIO). Therefore, USPIO-enhanced MR-VSI was performed on DU-4475, MDA-MB-435, and EOMA tumor–bearing mice xenografts with known differences in angiogenic activity and vessel morphology. MR results were compared to vessel sizes determined by immunohistochemistry (anti-CD31) and by intravital microscopy (IVM). MR-VSI revealed significantly different mean vessel sizes between the xenograft models at both USPIO doses (DU-4475: 20.6 ± 4.9 mm; MDA-MB-435: 37.4 ± 8.8 μm; and EOMA: 60.3 ± 9.6 μm at 80 μmol/kg; p < .05). Immunohistochemistry revealed lower values for all tumor entities, whereas the size distribution was in line with MR-measurements. IVM corroborated the MR results for DU-4475 and MDA-MB435, but showed similar vessel sizes for MDA-MB-435 and EOMA. Our MR-VSI method allowed a noninvasive estimation of the mean vessel size in mice xenograft solid tumors with variable vascularity using a clinically relevant USPIO dose range.https://doi.org/10.2310/7290.2013.00059 |
| spellingShingle | Thorsten Persigehl Janine Ring Tymoteusz Budny Anke Hahnenkamp Sandra Stoeppeler Lawrence H. Schwartz Hans-Ullrich Spiegel Walter Heindel Stefanie Remmele Christoph Bremer Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy Molecular Imaging |
| title | Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy |
| title_full | Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy |
| title_fullStr | Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy |
| title_full_unstemmed | Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy |
| title_short | Vessel Size Imaging (VSI) by Robust Magnetic Resonance (MR) Relaxometry: MR-VSI of Solid Tumors in Correlation with Immunohistology and Intravital Microscopy |
| title_sort | vessel size imaging vsi by robust magnetic resonance mr relaxometry mr vsi of solid tumors in correlation with immunohistology and intravital microscopy |
| url | https://doi.org/10.2310/7290.2013.00059 |
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