Comparison of reverse-transcriptase polymerase chain reaction (rt-pcr) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water
<b>Aim:</b> Coronaviruses and Rotaviruses are important virologic factors for both animal and human health in Turkey and the world. Bovine Rotavirus (BRV) and Bovine Coronavirus (BCoV) in cattle cause significant economic losses. The aim of this study was to determine the presence of BRV...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2020-10-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1292 |
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| Summary: | <b>Aim:</b> Coronaviruses and Rotaviruses are important virologic factors
for both animal and human health in Turkey and the world. Bovine
Rotavirus (BRV) and Bovine Coronavirus (BCoV) in cattle cause
significant economic losses. The aim of this study was to determine
the presence of BRV and BCoV in Anatolian buffaloes which were on
the same farms with cattle. For this purpose, presence of these two
viruses were investigated by Reverse Transcriptase Polymerase Chain
Reaction (RT-PCR) and BRV-BCoV Rapid tests and sensitivity and
specificity ratios of these two tests were compared.<p>
<b>Materials and Methods:</b> In this study, 230 Anatolian buffaloes were
clinically evaluated in cattle farms in Afyonkarahisar region. Fecal
samples were collected from 27 buffaloes which had clinical signs
(weakness, dehydration, vomiting, watery consistency and yellow
stool). The fecal samples were evaluated by Rapid Test and RT-PCR
for Bovine Rotavirus and Bovine Coronavirus. The analyzes were
performed according to the procedure of the commercial RT-PCR
and rapid kits.<p>
<b>Results: </b>The RT-PCR results were positive as 22.2% (6/27) for BRV
and 3.7% (1/27 27/1) for BcoV while Rota-Corona Rapid test results
were negative in all samples. When compared with RT-PCR results
for both viruses, the rapid test sensitivity and specificity was determined
as 0% and 100%, respectively. In addition, positive rates of
BRV was statistically important as BCoV rate in analyzed samples
(p<0,05).<p>
<b>Conclusion:</b> In conclusion, low sensitivity of rapid test may be due
to the change in the amount of virus scattered throughout the course
of enteric infections. |
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| ISSN: | 1309-6958 2146-1953 |