Host genetics maps to behaviour and brain structure in developmental mice
Abstract Gene-environment interactions in the postnatal period have a long-term impact on neurodevelopment. To effectively assess neurodevelopment in the mouse, we developed a behavioural pipeline that incorporates several validated behavioural tests to measure translationally relevant milestones of...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Behavioral and Brain Functions |
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| Online Access: | https://doi.org/10.1186/s12993-024-00261-y |
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| author | Sarah Asbury Jonathan K. Y. Lai Kelly C. Rilett Zeeshan Haqqee Benjamin C. Darwin Jacob Ellegood Jason P. Lerch Jane A. Foster |
| author_facet | Sarah Asbury Jonathan K. Y. Lai Kelly C. Rilett Zeeshan Haqqee Benjamin C. Darwin Jacob Ellegood Jason P. Lerch Jane A. Foster |
| author_sort | Sarah Asbury |
| collection | DOAJ |
| description | Abstract Gene-environment interactions in the postnatal period have a long-term impact on neurodevelopment. To effectively assess neurodevelopment in the mouse, we developed a behavioural pipeline that incorporates several validated behavioural tests to measure translationally relevant milestones of behaviour in mice. The behavioral phenotype of 1060 wild type and genetically-modified mice was examined followed by structural brain imaging at 4 weeks of age. The influence of genetics, sex, and early life stress on behaviour and neuroanatomy was determined using traditional statistical and machine learning methods. Analytical results demonstrated that neuroanatomical diversity was primarily associated with genotype whereas behavioural phenotypic diversity was observed to be more susceptible to gene-environment variation. We describe a standardized mouse phenotyping pipeline, termed the Developmental Behavioural Milestones (DBM) Pipeline released alongside the 1000 Mouse Developmental Behavioural Milestones (1000 Mouse DBM) database to institute a novel framework for reproducible interventional neuroscience research. |
| format | Article |
| id | doaj-art-b8ca18c6065b4fe395c1cdf41426edbf |
| institution | Kabale University |
| issn | 1744-9081 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Behavioral and Brain Functions |
| spelling | doaj-art-b8ca18c6065b4fe395c1cdf41426edbf2025-01-05T12:41:49ZengBMCBehavioral and Brain Functions1744-90812025-01-0121112010.1186/s12993-024-00261-yHost genetics maps to behaviour and brain structure in developmental miceSarah Asbury0Jonathan K. Y. Lai1Kelly C. Rilett2Zeeshan Haqqee3Benjamin C. Darwin4Jacob Ellegood5Jason P. Lerch6Jane A. Foster7Department of Psychiatry and Behavioural Neurosciences, McMaster UniversityDepartment of Psychiatry and Behavioural Neurosciences, McMaster UniversityDepartment of Psychiatry and Behavioural Neurosciences, McMaster UniversityDepartment of Psychiatry and Behavioural Neurosciences, McMaster UniversityMouse Imaging Centre, The Hospital for Sick ChildrenMouse Imaging Centre, The Hospital for Sick ChildrenMouse Imaging Centre, The Hospital for Sick ChildrenDepartment of Psychiatry and Behavioural Neurosciences, McMaster UniversityAbstract Gene-environment interactions in the postnatal period have a long-term impact on neurodevelopment. To effectively assess neurodevelopment in the mouse, we developed a behavioural pipeline that incorporates several validated behavioural tests to measure translationally relevant milestones of behaviour in mice. The behavioral phenotype of 1060 wild type and genetically-modified mice was examined followed by structural brain imaging at 4 weeks of age. The influence of genetics, sex, and early life stress on behaviour and neuroanatomy was determined using traditional statistical and machine learning methods. Analytical results demonstrated that neuroanatomical diversity was primarily associated with genotype whereas behavioural phenotypic diversity was observed to be more susceptible to gene-environment variation. We describe a standardized mouse phenotyping pipeline, termed the Developmental Behavioural Milestones (DBM) Pipeline released alongside the 1000 Mouse Developmental Behavioural Milestones (1000 Mouse DBM) database to institute a novel framework for reproducible interventional neuroscience research.https://doi.org/10.1186/s12993-024-00261-yNeurodevelopmentStructural MRIRandom forestMachine learningEarly life stress |
| spellingShingle | Sarah Asbury Jonathan K. Y. Lai Kelly C. Rilett Zeeshan Haqqee Benjamin C. Darwin Jacob Ellegood Jason P. Lerch Jane A. Foster Host genetics maps to behaviour and brain structure in developmental mice Behavioral and Brain Functions Neurodevelopment Structural MRI Random forest Machine learning Early life stress |
| title | Host genetics maps to behaviour and brain structure in developmental mice |
| title_full | Host genetics maps to behaviour and brain structure in developmental mice |
| title_fullStr | Host genetics maps to behaviour and brain structure in developmental mice |
| title_full_unstemmed | Host genetics maps to behaviour and brain structure in developmental mice |
| title_short | Host genetics maps to behaviour and brain structure in developmental mice |
| title_sort | host genetics maps to behaviour and brain structure in developmental mice |
| topic | Neurodevelopment Structural MRI Random forest Machine learning Early life stress |
| url | https://doi.org/10.1186/s12993-024-00261-y |
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