Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles
Background and Objectives: Airway fungal infection is a severe clinical problem, especially in patients with compromised immune functions. Here, we examined the distribution and antifungal susceptibility profiles of fungal agents isolated from respiratory tract of symptomatic patients hospitalized...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-12-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4825 |
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| author | Parviz Hassanpour Seyed Jamal Hashemi Sanam Nami Roshanak Daie Ghazvini Behrouz Naghili Hokmabadi Abbas Rahimi Foroushani Kazem Ahmadikia Zahra Ramezanalipour Saeid Firouzi Abriz |
| author_facet | Parviz Hassanpour Seyed Jamal Hashemi Sanam Nami Roshanak Daie Ghazvini Behrouz Naghili Hokmabadi Abbas Rahimi Foroushani Kazem Ahmadikia Zahra Ramezanalipour Saeid Firouzi Abriz |
| author_sort | Parviz Hassanpour |
| collection | DOAJ |
| description |
Background and Objectives: Airway fungal infection is a severe clinical problem, especially in patients with compromised immune functions. Here, we examined the distribution and antifungal susceptibility profiles of fungal agents isolated from respiratory tract of symptomatic patients hospitalized in pulmonary units.
Materials and Methods: This descriptive cross-sectional study took place from 2023 to 2024, involving 360 patients. Bronchoalveolar lavage (BAL) or sputum specimens were collected and analyzed using mycological and molecular methods for this study. Antifungal susceptibility testing (AFST) was carried out using the broth micro dilution method.
Results: Of a total of 360 respiratory specimens, 114 (31.6%) were positive. The male-to-female ratio was 63:51 (1.3%). Candida albicans and Aspergillus flavus were the most common yeast and mold species. Chronic obstructive pulmonary disease (COPD) had the highest rate of colonization with fungal agents (47/114, 41%). The isolates associated with COPD in this study included Aspergillus species (4/12, 3.5%), Candida species (41/96, 36%), and other fungal species (2/6, 1.5%). Coughing (87%) was the predominant symptom, and malignancy (52%) was the predominant comorbidity factor. The result of AFST for antifungal agents showed that 9 (22.5%) Candida isolates were resistant, and the highest rate of resistance was related to voriconazole agent (5/9, 55.5%). Resistance to antifungal agents was not observed among Aspergillus isolates.
Conclusion: This study showed a significant relationship between the frequency of Aspergillus and Candida species in patients with underlying lung diseases. In addition, voriconazole was more effective than itraconazole, especially against Aspergillus flavus.
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| format | Article |
| id | doaj-art-b8c0549dba284895973b54d620eec126 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-b8c0549dba284895973b54d620eec1262025-01-06T08:38:44ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472024-12-0116610.18502/ijm.v16i6.17258Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profilesParviz Hassanpour0Seyed Jamal Hashemi1Sanam Nami2Roshanak Daie Ghazvini3Behrouz Naghili Hokmabadi4Abbas Rahimi Foroushani5Kazem Ahmadikia6Zahra Ramezanalipour7Saeid Firouzi Abriz8Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranInfectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Background and Objectives: Airway fungal infection is a severe clinical problem, especially in patients with compromised immune functions. Here, we examined the distribution and antifungal susceptibility profiles of fungal agents isolated from respiratory tract of symptomatic patients hospitalized in pulmonary units. Materials and Methods: This descriptive cross-sectional study took place from 2023 to 2024, involving 360 patients. Bronchoalveolar lavage (BAL) or sputum specimens were collected and analyzed using mycological and molecular methods for this study. Antifungal susceptibility testing (AFST) was carried out using the broth micro dilution method. Results: Of a total of 360 respiratory specimens, 114 (31.6%) were positive. The male-to-female ratio was 63:51 (1.3%). Candida albicans and Aspergillus flavus were the most common yeast and mold species. Chronic obstructive pulmonary disease (COPD) had the highest rate of colonization with fungal agents (47/114, 41%). The isolates associated with COPD in this study included Aspergillus species (4/12, 3.5%), Candida species (41/96, 36%), and other fungal species (2/6, 1.5%). Coughing (87%) was the predominant symptom, and malignancy (52%) was the predominant comorbidity factor. The result of AFST for antifungal agents showed that 9 (22.5%) Candida isolates were resistant, and the highest rate of resistance was related to voriconazole agent (5/9, 55.5%). Resistance to antifungal agents was not observed among Aspergillus isolates. Conclusion: This study showed a significant relationship between the frequency of Aspergillus and Candida species in patients with underlying lung diseases. In addition, voriconazole was more effective than itraconazole, especially against Aspergillus flavus. https://ijm.tums.ac.ir/index.php/ijm/article/view/4825Lung infection;Underlying lung diseases;Antifungal;Fungal agents;Polymerase chain reaction;Iran |
| spellingShingle | Parviz Hassanpour Seyed Jamal Hashemi Sanam Nami Roshanak Daie Ghazvini Behrouz Naghili Hokmabadi Abbas Rahimi Foroushani Kazem Ahmadikia Zahra Ramezanalipour Saeid Firouzi Abriz Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles Iranian Journal of Microbiology Lung infection; Underlying lung diseases; Antifungal; Fungal agents; Polymerase chain reaction; Iran |
| title | Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles |
| title_full | Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles |
| title_fullStr | Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles |
| title_full_unstemmed | Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles |
| title_short | Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles |
| title_sort | distribution of fungal agents in the respiratory system of patients with underlying lung diseases molecular identification and antifungal susceptibility profiles |
| topic | Lung infection; Underlying lung diseases; Antifungal; Fungal agents; Polymerase chain reaction; Iran |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4825 |
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