Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources
Introduction: Campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis. Despite its public health importance, the virulence factors and mechanisms underlying C. jejuni pathogenesis remain poorly understood and the relationships between these genes and the sources of...
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The Journal of Infection in Developing Countries
2022-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/16485 |
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| author | Leonardo Moreira Lima Gustavo Perdoncini Karen Apellanis Borges Thales Quedi Furian Carlos Tadeu Pippi Salle Hamilton Luiz de Souza Moraes Vladimir Pinheiro do Nascimento |
| author_facet | Leonardo Moreira Lima Gustavo Perdoncini Karen Apellanis Borges Thales Quedi Furian Carlos Tadeu Pippi Salle Hamilton Luiz de Souza Moraes Vladimir Pinheiro do Nascimento |
| author_sort | Leonardo Moreira Lima |
| collection | DOAJ |
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Introduction: Campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis. Despite its public health importance, the virulence factors and mechanisms underlying C. jejuni pathogenesis remain poorly understood and the relationships between these genes and the sources of the strains are not clear. We aimed to determine the virulence profiles of C. jejuni isolated from poultry and human cases of Campylobacteriosis.
Methodology: A total of 50 strains of C. jejuni isolated from poultry and human cases of Campylobacteriosis were screened by polymerase chain reaction (PCR) for the presence of six pathogenic genes (flaA, iam, wlaN, cdtA, cdtB, cdtC).
Results: A total of 40% (10/25) of the human isolates presented only one virulence marker. In contrast, 64% (16/25) of the poultry-derived strains showed four or five virulence markers. cdtA and flaA occurred more frequently in poultry-derived strains than in human strains. Ten different virulence profiles were observed among the human isolates and 11 among the poultry strains. Only four profiles were common to both sources: profiles 3 (flaA, cdtA, cdtB, and cdtC), 5 (cdtA and cdtB), 7 (flaA and cdtB), and 10 (iam, flaA, cdtA, cdtB, and cdtC). The human-derived strains had a higher Shannon diversity index (1.9396) and Simpson index (0.8367), indicating a more diversified population than found in poultry (1.7742 and 0.7333, respectively).
Conclusions: We found variations in the genetic profiles of the circulating strains based on the isolation source and genes that are potentially pathogenic to humans were detected in poultry-derived strains.
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| format | Article |
| id | doaj-art-b8bfac726e384b059845b2d19a454efd |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-b8bfac726e384b059845b2d19a454efd2025-08-20T03:52:42ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-09-01160910.3855/jidc.16485Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sourcesLeonardo Moreira Lima0Gustavo Perdoncini1Karen Apellanis Borges2Thales Quedi Furian3Carlos Tadeu Pippi Salle4Hamilton Luiz de Souza Moraes5Vladimir Pinheiro do Nascimento6Centro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro de Diagnóstico e Pesquisa em Patologia Aviária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil Introduction: Campylobacter jejuni is one of the most common bacterial causes of human gastroenteritis. Despite its public health importance, the virulence factors and mechanisms underlying C. jejuni pathogenesis remain poorly understood and the relationships between these genes and the sources of the strains are not clear. We aimed to determine the virulence profiles of C. jejuni isolated from poultry and human cases of Campylobacteriosis. Methodology: A total of 50 strains of C. jejuni isolated from poultry and human cases of Campylobacteriosis were screened by polymerase chain reaction (PCR) for the presence of six pathogenic genes (flaA, iam, wlaN, cdtA, cdtB, cdtC). Results: A total of 40% (10/25) of the human isolates presented only one virulence marker. In contrast, 64% (16/25) of the poultry-derived strains showed four or five virulence markers. cdtA and flaA occurred more frequently in poultry-derived strains than in human strains. Ten different virulence profiles were observed among the human isolates and 11 among the poultry strains. Only four profiles were common to both sources: profiles 3 (flaA, cdtA, cdtB, and cdtC), 5 (cdtA and cdtB), 7 (flaA and cdtB), and 10 (iam, flaA, cdtA, cdtB, and cdtC). The human-derived strains had a higher Shannon diversity index (1.9396) and Simpson index (0.8367), indicating a more diversified population than found in poultry (1.7742 and 0.7333, respectively). Conclusions: We found variations in the genetic profiles of the circulating strains based on the isolation source and genes that are potentially pathogenic to humans were detected in poultry-derived strains. https://jidc.org/index.php/journal/article/view/16485BroilerCampylobacteriosisCampylobacter jejunihumanvirulence marker |
| spellingShingle | Leonardo Moreira Lima Gustavo Perdoncini Karen Apellanis Borges Thales Quedi Furian Carlos Tadeu Pippi Salle Hamilton Luiz de Souza Moraes Vladimir Pinheiro do Nascimento Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources Journal of Infection in Developing Countries Broiler Campylobacteriosis Campylobacter jejuni human virulence marker |
| title | Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources |
| title_full | Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources |
| title_fullStr | Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources |
| title_full_unstemmed | Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources |
| title_short | Prevalence and distribution of pathogenic genes in Campylobacter jejuni isolated from poultry and human sources |
| title_sort | prevalence and distribution of pathogenic genes in campylobacter jejuni isolated from poultry and human sources |
| topic | Broiler Campylobacteriosis Campylobacter jejuni human virulence marker |
| url | https://jidc.org/index.php/journal/article/view/16485 |
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