Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.

Gastrointestinal parasites (GIP) and mineral deficiencies are significant factors affecting health and productivity of free-ranging cattle. Adequate mineral intake, particularly from natural mineral water sources (hora), is vital for immune function, gastrointestinal health, and nutrient absorption....

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Main Authors: Ashenafi Miresa, Taye Tolemariam, Belay Duguma, Ellen S Dierenfeld, Abebe Nigussie, Feyissa Begna
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0329812
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author Ashenafi Miresa
Taye Tolemariam
Belay Duguma
Ellen S Dierenfeld
Abebe Nigussie
Feyissa Begna
author_facet Ashenafi Miresa
Taye Tolemariam
Belay Duguma
Ellen S Dierenfeld
Abebe Nigussie
Feyissa Begna
author_sort Ashenafi Miresa
collection DOAJ
description Gastrointestinal parasites (GIP) and mineral deficiencies are significant factors affecting health and productivity of free-ranging cattle. Adequate mineral intake, particularly from natural mineral water sources (hora), is vital for immune function, gastrointestinal health, and nutrient absorption. This study aimed to explore the association between GIP burden and serum mineral concentrations in zebu cattle (Bos indicus) routinely accessing hora mineral water in southwestern Ethiopia. A total of 180 fecal samples were collected from cattle across four districts (Bedele, Dabo, Gechi, and Borecha) and analyzed qualitatively and quantitatively to determine parasite presence and fecal egg count. Concurrently, blood samples were collected to evaluate serum mineral concentrations. The overall GIP prevalence was 55.6%, with Strongyle-type nematodes being the dominant GIP group. Gechi district showed the highest prevalence (64.4%) and mean egg per gram (EPG) of 212.8 (p < 0.05). Although, serum mineral concentrations were generally adequate, significant variations were observed across districts. Strong negative correlations (p < 0.05) were observed between EPG and serum concentrations of zinc (Zn), manganese (Mn), iron (Fe), and copper (Cu), indicating that adequate intake of these minerals, potentially sourced from the hora mineral water, may be associated with improved resistance to parasitic infections. These negative correlations were supported by negative binomial regression analysis which identified Zn as the strongest predictor of EPG. Overall, the findings highlight the importance of hora as a natural mineral supplement in its association with lower GIP burden in free-ranging zebu cattle. While this study indicates a correlation between serum mineral concentrations and GIP burden in grazing cattle, controlled experiments are essential to determine the specific effects of individual minerals found in hora on parasite resistance and establish causality.
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spelling doaj-art-b85f660733c84bddbf63b8dbcfc9f10d2025-08-23T05:32:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01208e032981210.1371/journal.pone.0329812Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.Ashenafi MiresaTaye TolemariamBelay DugumaEllen S DierenfeldAbebe NigussieFeyissa BegnaGastrointestinal parasites (GIP) and mineral deficiencies are significant factors affecting health and productivity of free-ranging cattle. Adequate mineral intake, particularly from natural mineral water sources (hora), is vital for immune function, gastrointestinal health, and nutrient absorption. This study aimed to explore the association between GIP burden and serum mineral concentrations in zebu cattle (Bos indicus) routinely accessing hora mineral water in southwestern Ethiopia. A total of 180 fecal samples were collected from cattle across four districts (Bedele, Dabo, Gechi, and Borecha) and analyzed qualitatively and quantitatively to determine parasite presence and fecal egg count. Concurrently, blood samples were collected to evaluate serum mineral concentrations. The overall GIP prevalence was 55.6%, with Strongyle-type nematodes being the dominant GIP group. Gechi district showed the highest prevalence (64.4%) and mean egg per gram (EPG) of 212.8 (p < 0.05). Although, serum mineral concentrations were generally adequate, significant variations were observed across districts. Strong negative correlations (p < 0.05) were observed between EPG and serum concentrations of zinc (Zn), manganese (Mn), iron (Fe), and copper (Cu), indicating that adequate intake of these minerals, potentially sourced from the hora mineral water, may be associated with improved resistance to parasitic infections. These negative correlations were supported by negative binomial regression analysis which identified Zn as the strongest predictor of EPG. Overall, the findings highlight the importance of hora as a natural mineral supplement in its association with lower GIP burden in free-ranging zebu cattle. While this study indicates a correlation between serum mineral concentrations and GIP burden in grazing cattle, controlled experiments are essential to determine the specific effects of individual minerals found in hora on parasite resistance and establish causality.https://doi.org/10.1371/journal.pone.0329812
spellingShingle Ashenafi Miresa
Taye Tolemariam
Belay Duguma
Ellen S Dierenfeld
Abebe Nigussie
Feyissa Begna
Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.
PLoS ONE
title Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.
title_full Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.
title_fullStr Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.
title_full_unstemmed Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.
title_short Association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing 'hora' mineral water in southwestern Ethiopia.
title_sort association between serum mineral concentrations and gastrointestinal parasite burden in zebu cattle accessing hora mineral water in southwestern ethiopia
url https://doi.org/10.1371/journal.pone.0329812
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