Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy
Microbial energy is a promising area of innovative development in bio- and nanotechnology. Recent studies have revealed that microbial communities of thermal springs have excellent implementation prospects in this area. The present article introduces the microbial diversity of the Abakan Arzhan ther...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2022-10-01
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| Series: | Техника и технология пищевых производств |
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| Online Access: | https://fptt.ru/en/issues/20632/20595/ |
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| author | Anastasiya I. Dmitrieva Elizaveta R. Faskhutdinova Margarita Yu. Drozdova Sergei S. Kutuzov Larisa A. Proskuryakova |
| author_facet | Anastasiya I. Dmitrieva Elizaveta R. Faskhutdinova Margarita Yu. Drozdova Sergei S. Kutuzov Larisa A. Proskuryakova |
| author_sort | Anastasiya I. Dmitrieva |
| collection | DOAJ |
| description | Microbial energy is a promising area of innovative development in bio- and nanotechnology. Recent studies have revealed that microbial communities of thermal springs have excellent implementation prospects in this area. The present article introduces the microbial diversity of the Abakan Arzhan thermal spring and their isolates that are potentially applicable in microbial electricity synthesis.
The research featured microbial isolates obtained from a microbiota analysis of water and slit samples from the Abakan Arzhan thermal spring. The study involved a metagenomic analysis of the microbial community, as well as such molecular biology methods as nucleic acid extraction, PCR, sequencing, phylogenetic, and bioinformatic analysis. The Silva library was used to compare 16S RNA sequences
Firmicutes, Bacteroides, and Proteobacteria proved to be the dominant phylotypes for water samples, while Firmicutes, Thermomonas, Gammaproteobacteria, and Proteobacteria were the dominant phylotypes for slit samples. The analysis of minor phylotypes confirmed the presence of Geobacter and Shewanella in the samples. The total number of obtained enrichment cultures was nine. Two types of resistant colonies were discovered during the isolation of extremophilic iron-reducing isolates. The samples were grown on a medium containing iron (III) acetate and iron (III) nitrate, and the isolates appeared to be in the process of Fe(III) reduction. The isolates showed an intense iron recovery of 409 and 407 µg/mL after 72 h of cultivation.
The study confirmed the ability of the acquired isolates to reduce iron, making them a priority for future microbial energy research. The isolates belonged to the Shewanella algae and Geobacter sulfurreducens species, as determined by 16S RNA morphology and phylogenetic analyses. |
| format | Article |
| id | doaj-art-b859675e34d74d2fbdda1b99c34d2dec |
| institution | Kabale University |
| issn | 2074-9414 2313-1748 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj-art-b859675e34d74d2fbdda1b99c34d2dec2024-12-02T03:58:29ZengKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482022-10-0152245846810.21603/2074-9414-2022-3-2384Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial EnergyAnastasiya I. Dmitrieva0https://orcid.org/0000-0002-8764-4049Elizaveta R. Faskhutdinova1https://orcid.org/0000-0001-9711-2145Margarita Yu. Drozdova2https://orcid.org/0000-0002-9499-9470Sergei S. Kutuzov3https://orcid.org/0000-0001-8807-5573Larisa A. Proskuryakova4https://orcid.org/0000-0002-9583-9161Kemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaKemerovo State University, Kemerovo, RussiaMiddle School of General Education №34, Krasnobrodsky, RussiaKemerovo State University, Kemerovo, RussiaMicrobial energy is a promising area of innovative development in bio- and nanotechnology. Recent studies have revealed that microbial communities of thermal springs have excellent implementation prospects in this area. The present article introduces the microbial diversity of the Abakan Arzhan thermal spring and their isolates that are potentially applicable in microbial electricity synthesis. The research featured microbial isolates obtained from a microbiota analysis of water and slit samples from the Abakan Arzhan thermal spring. The study involved a metagenomic analysis of the microbial community, as well as such molecular biology methods as nucleic acid extraction, PCR, sequencing, phylogenetic, and bioinformatic analysis. The Silva library was used to compare 16S RNA sequences Firmicutes, Bacteroides, and Proteobacteria proved to be the dominant phylotypes for water samples, while Firmicutes, Thermomonas, Gammaproteobacteria, and Proteobacteria were the dominant phylotypes for slit samples. The analysis of minor phylotypes confirmed the presence of Geobacter and Shewanella in the samples. The total number of obtained enrichment cultures was nine. Two types of resistant colonies were discovered during the isolation of extremophilic iron-reducing isolates. The samples were grown on a medium containing iron (III) acetate and iron (III) nitrate, and the isolates appeared to be in the process of Fe(III) reduction. The isolates showed an intense iron recovery of 409 and 407 µg/mL after 72 h of cultivation. The study confirmed the ability of the acquired isolates to reduce iron, making them a priority for future microbial energy research. The isolates belonged to the Shewanella algae and Geobacter sulfurreducens species, as determined by 16S RNA morphology and phylogenetic analyses.https://fptt.ru/en/issues/20632/20595/microbial energymicrobial electrosynthesismicrobial fuel cellextremophilic microorganismsmicrobiotathermal springs |
| spellingShingle | Anastasiya I. Dmitrieva Elizaveta R. Faskhutdinova Margarita Yu. Drozdova Sergei S. Kutuzov Larisa A. Proskuryakova Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy Техника и технология пищевых производств microbial energy microbial electrosynthesis microbial fuel cell extremophilic microorganisms microbiota thermal springs |
| title | Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy |
| title_full | Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy |
| title_fullStr | Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy |
| title_full_unstemmed | Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy |
| title_short | Phylogenetic Diversity of Microorganisms from the Abakan Arzhan Thermal Spring: Potential Producers of Microbial Energy |
| title_sort | phylogenetic diversity of microorganisms from the abakan arzhan thermal spring potential producers of microbial energy |
| topic | microbial energy microbial electrosynthesis microbial fuel cell extremophilic microorganisms microbiota thermal springs |
| url | https://fptt.ru/en/issues/20632/20595/ |
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