Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma
Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/6686625 |
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| author | Sue Jean Mun Shin Ae Kang Hye-Kyung Park Hak Sun Yu Kyu-Sup Cho Hwan-Jung Roh |
| author_facet | Sue Jean Mun Shin Ae Kang Hye-Kyung Park Hak Sun Yu Kyu-Sup Cho Hwan-Jung Roh |
| author_sort | Sue Jean Mun |
| collection | DOAJ |
| description | Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p<0.001), and the serum total and OVA-specific immunoglobulin (Ig)E (p=0.048 and p=0.001) and total IgG1 (p<0.001). Interleukin- (IL-) 4 was significantly inhibited with ASC-derived EV pretreatment in the BALF and lung draining lymph nodes (LLNs) (p=0.040 and p=0.011). Furthermore, ASC-derived EV administration resulted in a significant increase of the regulatory T cell (Treg) populations in LLNs. ASC-derived EVs alleviated AHR and allergic airway inflammation caused by the induction of Treg expansion in a mouse model of asthma. There seems to be a role for ASC-derived EVs as a modifier in allergic airway disease. |
| format | Article |
| id | doaj-art-b85443e15a274f88ab34266920ee2fce |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b85443e15a274f88ab34266920ee2fce2025-02-03T07:23:30ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/66866256686625Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of AsthmaSue Jean Mun0Shin Ae Kang1Hye-Kyung Park2Hak Sun Yu3Kyu-Sup Cho4Hwan-Jung Roh5Department of Otorhinolaryngology, Pusan National University Yangsan Hospital, Yangsan, Republic of KoreaDepartment of Parasitology and Tropical Medicine, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Internal Medicine, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Parasitology and Tropical Medicine, Pusan National University School of Medicine, Yangsan, Republic of KoreaDepartment of Otorhinolaryngology, Pusan National University Hospital, Busan, Republic of KoreaDepartment of Otorhinolaryngology, Pusan National University Yangsan Hospital, Yangsan, Republic of KoreaAsthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p<0.001), and the serum total and OVA-specific immunoglobulin (Ig)E (p=0.048 and p=0.001) and total IgG1 (p<0.001). Interleukin- (IL-) 4 was significantly inhibited with ASC-derived EV pretreatment in the BALF and lung draining lymph nodes (LLNs) (p=0.040 and p=0.011). Furthermore, ASC-derived EV administration resulted in a significant increase of the regulatory T cell (Treg) populations in LLNs. ASC-derived EVs alleviated AHR and allergic airway inflammation caused by the induction of Treg expansion in a mouse model of asthma. There seems to be a role for ASC-derived EVs as a modifier in allergic airway disease.http://dx.doi.org/10.1155/2021/6686625 |
| spellingShingle | Sue Jean Mun Shin Ae Kang Hye-Kyung Park Hak Sun Yu Kyu-Sup Cho Hwan-Jung Roh Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma Stem Cells International |
| title | Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma |
| title_full | Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma |
| title_fullStr | Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma |
| title_full_unstemmed | Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma |
| title_short | Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma |
| title_sort | intranasally administered extracellular vesicles from adipose stem cells have immunomodulatory effects in a mouse model of asthma |
| url | http://dx.doi.org/10.1155/2021/6686625 |
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