Time as a significant factor in the release of potassium from lithium heparin plasma and serum.
<h4>Objectives</h4>In most countries the majority of patients are in outpatient care. In difference to hospitalized patients, their blood samples often take hours after collection to centrifugation. The study investigates the release of potassium and the development of pseudohyperkalemia...
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Public Library of Science (PLoS)
2024-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0313572 |
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| author | Tom Reuter Michael Müller Felix Stelter Jürgen Durner Jan Kramer |
| author_facet | Tom Reuter Michael Müller Felix Stelter Jürgen Durner Jan Kramer |
| author_sort | Tom Reuter |
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| description | <h4>Objectives</h4>In most countries the majority of patients are in outpatient care. In difference to hospitalized patients, their blood samples often take hours after collection to centrifugation. The study investigates the release of potassium and the development of pseudohyperkalemia in lithium heparin (Li-Hep) and serum blood collection tubes over time.<h4>Methods</h4>From 201 donors 4 serum and 4 Li-Hep blood collection tubes were taken each. After 0.5, 4, 6 and 8h whole blood was centrifuged, and potassium levels were determined. To simulate the preanalytic conditions, the samples with a storage time >0.5h were shaken on a standard shaker for 1h and stored at 4-8°C for the remaining time.<h4>Results</h4>Over time, significant more potassium was released before centrifugation from the Li-Hep plasma than from serum (1.21 vs 0.94 mmol/L). After 6h, the two groups were no longer highly statistically significantly different (potassium mean: 5.01 mmol/L in serum group, 4.92 mmol/L in Li-Hep group). In the Li-Hep group 164 donors developed a pseudohyperkalemia after 8h, compared to 76 in the serum group.<h4>Significance</h4>The decision as to which material is best suited should not only be based on which value comes closest to the physiological situation immediately after blood collection. The subsequent preanalytic circumstances must also be considered. Serum tubes appear to be at least as suitable for potassium determination as Li-Hep tubes. In terms of patient blood management, serum provides the possibility of performing a wider range of analyses in the outpatient setting. |
| format | Article |
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| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-b849eafcf4924b5dbff1a3469e6c2e1e2024-12-11T05:31:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031357210.1371/journal.pone.0313572Time as a significant factor in the release of potassium from lithium heparin plasma and serum.Tom ReuterMichael MüllerFelix StelterJürgen DurnerJan Kramer<h4>Objectives</h4>In most countries the majority of patients are in outpatient care. In difference to hospitalized patients, their blood samples often take hours after collection to centrifugation. The study investigates the release of potassium and the development of pseudohyperkalemia in lithium heparin (Li-Hep) and serum blood collection tubes over time.<h4>Methods</h4>From 201 donors 4 serum and 4 Li-Hep blood collection tubes were taken each. After 0.5, 4, 6 and 8h whole blood was centrifuged, and potassium levels were determined. To simulate the preanalytic conditions, the samples with a storage time >0.5h were shaken on a standard shaker for 1h and stored at 4-8°C for the remaining time.<h4>Results</h4>Over time, significant more potassium was released before centrifugation from the Li-Hep plasma than from serum (1.21 vs 0.94 mmol/L). After 6h, the two groups were no longer highly statistically significantly different (potassium mean: 5.01 mmol/L in serum group, 4.92 mmol/L in Li-Hep group). In the Li-Hep group 164 donors developed a pseudohyperkalemia after 8h, compared to 76 in the serum group.<h4>Significance</h4>The decision as to which material is best suited should not only be based on which value comes closest to the physiological situation immediately after blood collection. The subsequent preanalytic circumstances must also be considered. Serum tubes appear to be at least as suitable for potassium determination as Li-Hep tubes. In terms of patient blood management, serum provides the possibility of performing a wider range of analyses in the outpatient setting.https://doi.org/10.1371/journal.pone.0313572 |
| spellingShingle | Tom Reuter Michael Müller Felix Stelter Jürgen Durner Jan Kramer Time as a significant factor in the release of potassium from lithium heparin plasma and serum. PLoS ONE |
| title | Time as a significant factor in the release of potassium from lithium heparin plasma and serum. |
| title_full | Time as a significant factor in the release of potassium from lithium heparin plasma and serum. |
| title_fullStr | Time as a significant factor in the release of potassium from lithium heparin plasma and serum. |
| title_full_unstemmed | Time as a significant factor in the release of potassium from lithium heparin plasma and serum. |
| title_short | Time as a significant factor in the release of potassium from lithium heparin plasma and serum. |
| title_sort | time as a significant factor in the release of potassium from lithium heparin plasma and serum |
| url | https://doi.org/10.1371/journal.pone.0313572 |
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