Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells
There is increasing evidence of the health effects of germinated colored rice (GCR). Metastasis, namely the spread of cancer cells to distant sites in the body, is a serious problem for cancer therapy. This study aimed to explore the anti-metastatic potential of GCR extract in HT29 colorectal cancer...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006583 |
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| author | Kittirat Saharat Natthaphorn Paramee N. Monique Paricharttanakul Nuchanart Rangkadilok Daranee Chokchaichamnankit Chantragan Srisomsap Kriengsak Lirdprapamongkol Jisnuson Svasti Jutamaad Satayavivad |
| author_facet | Kittirat Saharat Natthaphorn Paramee N. Monique Paricharttanakul Nuchanart Rangkadilok Daranee Chokchaichamnankit Chantragan Srisomsap Kriengsak Lirdprapamongkol Jisnuson Svasti Jutamaad Satayavivad |
| author_sort | Kittirat Saharat |
| collection | DOAJ |
| description | There is increasing evidence of the health effects of germinated colored rice (GCR). Metastasis, namely the spread of cancer cells to distant sites in the body, is a serious problem for cancer therapy. This study aimed to explore the anti-metastatic potential of GCR extract in HT29 colorectal cancer cells through cell migration inhibition, and to identify target proteins by using proteomic approach. GCR extract at 4 mg/mL caused 87 % reduction in cell migration. Two-dimensional gel proteomic analysis revealed β-actin downregulation in GCR-treated cells. GCR also affected gene expression of actin dynamic regulatory proteins including RHOA, RAC1, and CDC42 genes. Gamma-aminobutyric acid (GABA), a bioactive compound present in GCR extract, could decrease cell migration and downregulated β-actin in the cells, but did not alter expression level of RHOA, RAC1, and CDC42 genes. This study reveals a potential of GCR to be developed as a functional food for assisting the cancer treatment. |
| format | Article |
| id | doaj-art-b82e288629d04662b96678459ff8454c |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-b82e288629d04662b96678459ff8454c2025-01-12T05:24:48ZengElsevierJournal of Functional Foods1756-46462025-01-01124106655Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cellsKittirat Saharat0Natthaphorn Paramee1N. Monique Paricharttanakul2Nuchanart Rangkadilok3Daranee Chokchaichamnankit4Chantragan Srisomsap5Kriengsak Lirdprapamongkol6Jisnuson Svasti7Jutamaad Satayavivad8Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; Cambridge Institute for Medical Research, University of Cambridge, Cambridge, CB2 0XY, UKLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Bangkok 10400, ThailandCenter of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Bangkok 10400, Thailand; Laboratory of Pharmacology, Chulabhorn Research Institute, Bangkok 10210, Thailand; Corresponding authors.Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand; Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI, Bangkok 10400, Thailand; Corresponding authors.Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, ThailandLaboratory of Pharmacology, Chulabhorn Research Institute, Bangkok 10210, ThailandThere is increasing evidence of the health effects of germinated colored rice (GCR). Metastasis, namely the spread of cancer cells to distant sites in the body, is a serious problem for cancer therapy. This study aimed to explore the anti-metastatic potential of GCR extract in HT29 colorectal cancer cells through cell migration inhibition, and to identify target proteins by using proteomic approach. GCR extract at 4 mg/mL caused 87 % reduction in cell migration. Two-dimensional gel proteomic analysis revealed β-actin downregulation in GCR-treated cells. GCR also affected gene expression of actin dynamic regulatory proteins including RHOA, RAC1, and CDC42 genes. Gamma-aminobutyric acid (GABA), a bioactive compound present in GCR extract, could decrease cell migration and downregulated β-actin in the cells, but did not alter expression level of RHOA, RAC1, and CDC42 genes. This study reveals a potential of GCR to be developed as a functional food for assisting the cancer treatment.http://www.sciencedirect.com/science/article/pii/S1756464624006583Germinated colored riceFunctional foodColorectal cancerProteomicsCell migration inhibitionβ-Actin |
| spellingShingle | Kittirat Saharat Natthaphorn Paramee N. Monique Paricharttanakul Nuchanart Rangkadilok Daranee Chokchaichamnankit Chantragan Srisomsap Kriengsak Lirdprapamongkol Jisnuson Svasti Jutamaad Satayavivad Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells Journal of Functional Foods Germinated colored rice Functional food Colorectal cancer Proteomics Cell migration inhibition β-Actin |
| title | Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells |
| title_full | Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells |
| title_fullStr | Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells |
| title_full_unstemmed | Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells |
| title_short | Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells |
| title_sort | unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells |
| topic | Germinated colored rice Functional food Colorectal cancer Proteomics Cell migration inhibition β-Actin |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006583 |
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